Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilB/pilB1   Type   Machinery gene
Locus tag   CleRt_RS08685 Genome accession   NZ_CP011126
Coordinates   167779..167952 (+) Length   57 a.a.
NCBI ID   WP_082160388.1    Uniprot ID   -
Organism   Candidatus Coxiella mudrowiae strain CRt     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 162779..172952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CleRt_RS00750 (CleRT_01510) pyk 163175..164638 (+) 1464 WP_082160385.1 pyruvate kinase -
  CleRt_RS00755 (CleRT_01520) fba 164656..165738 (+) 1083 WP_048874823.1 class II fructose-bisphosphate aldolase -
  CleRt_RS00760 - 165954..166154 (+) 201 WP_048874824.1 secretin N-terminal domain-containing protein -
  CleRt_RS11130 - 166323..166783 (+) 461 Protein_192 type II secretion system protein GspD -
  CleRt_RS00770 - 166817..167023 (+) 207 WP_048874826.1 hypothetical protein -
  CleRt_RS09660 - 167290..167430 (+) 141 WP_157870759.1 hypothetical protein -
  CleRt_RS13815 pilB 167601..167750 (+) 150 WP_157870760.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  CleRt_RS08685 pilB/pilB1 167779..167952 (+) 174 WP_082160388.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  CleRt_RS13820 - 167967..168044 (+) 78 WP_157870941.1 hypothetical protein -
  CleRt_RS13825 - 168052..168255 (+) 204 Protein_198 ATPase, T2SS/T4P/T4SS family -
  CleRt_RS08160 - 168225..168548 (+) 324 WP_053097813.1 hypothetical protein -
  CleRt_RS08690 - 169372..169773 (+) 402 WP_306686886.1 type II secretion system F family protein -
  CleRt_RS13830 pilA/pilA1 169821..169922 (+) 102 WP_371440240.1 type IV pilin protein Machinery gene
  CleRt_RS13835 - 169966..170145 (+) 180 WP_371440241.1 type II secretion system protein GspG -
  CleRt_RS09680 - 170284..170427 (+) 144 WP_157870764.1 hypothetical protein -
  CleRt_RS08695 - 170740..170880 (+) 141 Protein_204 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CleRt_RS00795 - 171089..171412 (+) 324 WP_048874828.1 type II secretion system protein J -
  CleRt_RS00800 - 171477..171752 (+) 276 WP_048874829.1 type II secretion system protein GspJ -
  CleRt_RS12865 - 171862..171993 (+) 132 WP_306686721.1 hypothetical protein -
  CleRt_RS00805 - 172140..172343 (+) 204 WP_162198803.1 type II secretion system protein GspK -
  CleRt_RS09685 - 172416..172556 (+) 141 WP_157870765.1 hypothetical protein -

Sequence


Protein


Download         Length: 57 a.a.        Molecular weight: 6215.17 Da        Isoelectric Point: 8.4739

>NTDB_id=142572 CleRt_RS08685 WP_082160388.1 167779..167952(+) (pilB/pilB1) [Candidatus Coxiella mudrowiae strain CRt]
MNFKTHGIILVTSPYTGSGKTTTLYAALTALNSVVRNILTVEDPIEYYLPQAGQTLK

Nucleotide


Download         Length: 174 bp        

>NTDB_id=142572 CleRt_RS08685 WP_082160388.1 167779..167952(+) (pilB/pilB1) [Candidatus Coxiella mudrowiae strain CRt]
ATTAATTTTAAAACCCACGGTATTATTTTAGTGACCAGCCCCTACACGGGTTCGGGAAAAACAACCACTTTATATGCAGC
ATTAACAGCATTGAACAGCGTAGTGCGAAATATTTTAACGGTTGAAGATCCCATTGAATACTATTTACCTCAAGCAGGAC
AGACTCTCAAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

56.863

89.474

0.509

  pilB Legionella pneumophila strain ERS1305867

54.902

89.474

0.491

  ctsE Campylobacter jejuni subsp. jejuni 81-176

52.83

92.982

0.491

  pilB Vibrio campbellii strain DS40M4

52.941

89.474

0.474

  pilT Acinetobacter baumannii D1279779

61.905

73.684

0.456

  pilT Acinetobacter baumannii strain A118

61.905

73.684

0.456

  pilT Acinetobacter nosocomialis M2

61.905

73.684

0.456

  pilF Neisseria gonorrhoeae MS11

50.98

89.474

0.456

  pilB Acinetobacter baumannii D1279779

49.02

89.474

0.439

  pilB Glaesserella parasuis strain SC1401

58.14

75.439

0.439

  pilB Vibrio parahaemolyticus RIMD 2210633

49.02

89.474

0.439

  pilB Acinetobacter baylyi ADP1

49.02

89.474

0.439

  pilB Vibrio cholerae strain A1552

49.02

89.474

0.439

  pilT Pseudomonas aeruginosa PAK

59.524

73.684

0.439

  pilT Acinetobacter baylyi ADP1

59.524

73.684

0.439

  pilT Pseudomonas stutzeri DSM 10701

59.524

73.684

0.439

  pilB Haemophilus influenzae Rd KW20

54.348

80.702

0.439

  pilB Haemophilus influenzae 86-028NP

54.348

80.702

0.439

  pilT Legionella pneumophila strain ERS1305867

56.818

77.193

0.439

  pilT Legionella pneumophila strain Lp02

56.818

77.193

0.439

  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.231

91.228

0.404

  pilF Thermus thermophilus HB27

44.231

91.228

0.404

  pilT Neisseria gonorrhoeae MS11

54.762

73.684

0.404

  pilT Neisseria meningitidis 8013

54.762

73.684

0.404

  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.762

73.684

0.404

  pilT Vibrio cholerae strain A1552

54.762

73.684

0.404

  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

80.702

0.404

  comGA Bacillus subtilis subsp. subtilis str. 168

53.488

75.439

0.404

  pilU Vibrio cholerae strain A1552

53.488

75.439

0.404


Multiple sequence alignment