Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilB   Type   Machinery gene
Locus tag   CleRt_RS13815 Genome accession   NZ_CP011126
Coordinates   167601..167750 (+) Length   49 a.a.
NCBI ID   WP_157870760.1    Uniprot ID   -
Organism   Candidatus Coxiella mudrowiae strain CRt     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 162601..172750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CleRt_RS00750 (CleRT_01510) pyk 163175..164638 (+) 1464 WP_082160385.1 pyruvate kinase -
  CleRt_RS00755 (CleRT_01520) fba 164656..165738 (+) 1083 WP_048874823.1 class II fructose-bisphosphate aldolase -
  CleRt_RS00760 - 165954..166154 (+) 201 WP_048874824.1 secretin N-terminal domain-containing protein -
  CleRt_RS11130 - 166323..166783 (+) 461 Protein_192 type II secretion system protein GspD -
  CleRt_RS00770 - 166817..167023 (+) 207 WP_048874826.1 hypothetical protein -
  CleRt_RS09660 - 167290..167430 (+) 141 WP_157870759.1 hypothetical protein -
  CleRt_RS13815 pilB 167601..167750 (+) 150 WP_157870760.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  CleRt_RS08685 pilB/pilB1 167779..167952 (+) 174 WP_082160388.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  CleRt_RS13820 - 167967..168044 (+) 78 WP_157870941.1 hypothetical protein -
  CleRt_RS13825 - 168052..168255 (+) 204 Protein_198 ATPase, T2SS/T4P/T4SS family -
  CleRt_RS08160 - 168225..168548 (+) 324 WP_053097813.1 hypothetical protein -
  CleRt_RS08690 - 169372..169773 (+) 402 WP_306686886.1 type II secretion system F family protein -
  CleRt_RS13830 pilA/pilA1 169821..169922 (+) 102 WP_371440240.1 type IV pilin protein Machinery gene
  CleRt_RS13835 - 169966..170145 (+) 180 WP_371440241.1 type II secretion system protein GspG -
  CleRt_RS09680 - 170284..170427 (+) 144 WP_157870764.1 hypothetical protein -
  CleRt_RS08695 - 170740..170880 (+) 141 Protein_204 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CleRt_RS00795 - 171089..171412 (+) 324 WP_048874828.1 type II secretion system protein J -
  CleRt_RS00800 - 171477..171752 (+) 276 WP_048874829.1 type II secretion system protein GspJ -
  CleRt_RS12865 - 171862..171993 (+) 132 WP_306686721.1 hypothetical protein -
  CleRt_RS00805 - 172140..172343 (+) 204 WP_162198803.1 type II secretion system protein GspK -
  CleRt_RS09685 - 172416..172556 (+) 141 WP_157870765.1 hypothetical protein -

Sequence


Protein


Download         Length: 49 a.a.        Molecular weight: 5601.58 Da        Isoelectric Point: 10.2267

>NTDB_id=142576 CleRt_RS13815 WP_157870760.1 167601..167750(+) (pilB) [Candidatus Coxiella mudrowiae strain CRt]
MPQDGRIYLRIAGQAVDVRVSTIPTHFGERVVLRLLDKQSAHLICKNWV

Nucleotide


Download         Length: 150 bp        

>NTDB_id=142576 CleRt_RS13815 WP_157870760.1 167601..167750(+) (pilB) [Candidatus Coxiella mudrowiae strain CRt]
TTGCCTCAGGATGGCCGTATTTATCTGCGAATTGCTGGTCAGGCCGTTGATGTTCGAGTATCTACAATTCCTACACACTT
CGGTGAGCGTGTCGTTTTGCGTTTGTTGGATAAACAGAGCGCCCATTTGATTTGCAAAAACTGGGTATGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

61.364

89.796

0.551

  pilB Vibrio campbellii strain DS40M4

61.364

89.796

0.551

  pilB Vibrio cholerae strain A1552

59.091

89.796

0.531

  pilF Neisseria gonorrhoeae MS11

53.333

91.837

0.49

  pilB Acinetobacter baumannii D1279779

54.545

89.796

0.49

  pilB Acinetobacter baylyi ADP1

52.273

89.796

0.469

  pilB Legionella pneumophila strain ERS1305867

50

89.796

0.449

  pilF Thermus thermophilus HB27

52.5

81.633

0.429

  pilB/pilB1 Synechocystis sp. PCC 6803

47.619

85.714

0.408

  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.5

81.633

0.388


Multiple sequence alignment