Detailed information    

experimental Experimentally validated

Overview

Name   ctsE   Type   Machinery gene
Locus tag   CJJ81176_RS07050 Genome accession   NC_008787
Coordinates   1383246..1384805 (-) Length   519 a.a.
NCBI ID   WP_011812800.1    Uniprot ID   A0A5T0G0P4
Organism   Campylobacter jejuni subsp. jejuni 81-176     
Function   type II secretion system/type IV pilin; DNA uptake   
DNA binding and uptake

Function

Mutants of C. jejuni lacking the PSTC proteins CtsD, CtsE, CtsF, and CtsG decrease DNA uptake to background levels.


Genomic Context

Location: 1378246..1389805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJJ81176_RS07025 (CJJ81176_1458) - 1378256..1378690 (+) 435 WP_002779263.1 flagellar protein FlgN -
  CJJ81176_RS07030 (CJJ81176_1459) flgK 1378700..1380526 (+) 1827 WP_002869196.1 flagellar hook-associated protein FlgK -
  CJJ81176_RS07035 (CJJ81176_1460) - 1380543..1381306 (+) 764 Protein_1353 TIGR02757 family protein -
  CJJ81176_RS07040 (CJJ81176_1462) - 1381310..1382074 (+) 765 WP_002855301.1 TSUP family transporter -
  CJJ81176_RS07045 (CJJ81176_1463) ctsF 1382071..1383249 (-) 1179 WP_002869197.1 type II secretion system F family protein Machinery gene
  CJJ81176_RS07050 (CJJ81176_1464) ctsE 1383246..1384805 (-) 1560 WP_011812800.1 GspE/PulE family protein Machinery gene
  CJJ81176_RS07055 (CJJ81176_1465) ctsX 1384795..1385382 (-) 588 WP_002851370.1 membrane protein Machinery gene
  CJJ81176_RS07060 (CJJ81176_1466) ctsP 1385386..1385994 (-) 609 WP_011812801.1 ATP-binding protein Machinery gene
  CJJ81176_RS07065 (CJJ81176_1467) ctsD 1385987..1387399 (-) 1413 WP_002856543.1 pilus (MSHA type) biogenesis protein MshL Machinery gene
  CJJ81176_RS07070 (CJJ81176_1468) ctsR 1387377..1387688 (-) 312 WP_002855438.1 hypothetical protein Machinery gene

Sequence

Protein


Download         Length: 519 a.a.        Molecular weight: 60132.95 Da        Isoelectric Point: 5.8204

>NTDB_id=1227 CJJ81176_RS07050 WP_011812800.1 1383246..1384805(-) (ctsE) [Campylobacter jejuni subsp. jejuni 81-176]
MESRMDKIFQAYIDNEISLDEICTKFNITSWDFFKKLANFCNLHFVDLDEDNDFIYEGIPFSLLLKFKFLLIKNNDGFMI
IRSKPCSLELLEQVKTFMICEKIDTAIADELKIAKILNQIRIQEEIKRLSIKLRLEWQENHKRDDQSCISQIFDFLLHEI
LSFHASDIHIEARNDDALIRFRVDGILREFAIFEKDIYEALVFHVKFLACLNLAESRKTQDGSFELDFENERYDFRVSCL
PLIYGESVVIRILKHDKEILDLYKLNLGNKNLEILKKILHRPNGMILLTGPTGSGKSTTLYACLNELKSIEKKIISAEDP
IEYKIPLVQQILLNSKVGVEFNSVLRAILRQDPDIIMIGEIRDEESLDIALKASLTGHLLLSTLHTNDALSTIDRLLDMQ
AKSYLIASALSLVIAQRLVRKLCPWCKQKSKKHYIEFEGEFFEPKGCERCHHSGFFGRELIAECLEINEDLACAIRENQD
KTILMELAKKYGFQTMFEQGLKKAKEGLTSIDELLRVVR

Nucleotide


Download         Length: 1560 bp        

>NTDB_id=1227 CJJ81176_RS07050 WP_011812800.1 1383246..1384805(-) (ctsE) [Campylobacter jejuni subsp. jejuni 81-176]
ATGGAAAGTAGAATGGATAAAATTTTTCAAGCTTATATTGATAATGAAATAAGTCTTGATGAAATTTGCACTAAATTTAA
TATCACTTCTTGGGATTTTTTTAAAAAATTAGCAAATTTTTGTAATTTGCATTTTGTTGATTTAGATGAAGATAATGATT
TTATTTATGAAGGTATACCTTTTTCTTTACTTTTAAAATTTAAATTTCTGCTTATTAAAAACAATGACGGCTTTATGATT
ATTAGATCCAAACCTTGTTCTTTAGAGCTTTTAGAGCAAGTTAAGACTTTTATGATTTGTGAAAAAATCGACACTGCAAT
TGCCGATGAATTAAAGATAGCTAAAATTTTAAATCAAATTCGCATTCAAGAGGAGATTAAAAGATTAAGCATTAAGCTTA
GACTTGAATGGCAAGAAAATCACAAAAGAGATGATCAAAGTTGCATCAGTCAAATTTTTGATTTTTTATTACATGAAATT
TTATCTTTTCATGCGAGTGATATTCATATAGAAGCAAGGAATGATGATGCTTTGATTAGGTTTAGGGTTGATGGAATTTT
GCGTGAATTTGCTATTTTTGAGAAGGATATTTATGAAGCTTTGGTTTTTCATGTCAAATTTTTAGCTTGTTTGAATTTAG
CTGAATCAAGAAAAACACAAGATGGAAGTTTTGAATTAGATTTTGAAAATGAAAGATATGATTTTCGTGTTTCATGTTTG
CCTTTGATTTATGGCGAAAGTGTTGTTATAAGAATTTTAAAACATGATAAAGAAATTTTGGATTTGTATAAACTAAATCT
TGGAAATAAAAATTTAGAAATTTTAAAAAAGATTTTACATCGTCCCAATGGAATGATTTTATTAACCGGTCCTACAGGTA
GTGGTAAAAGCACAACTTTATACGCGTGTTTAAATGAGTTGAAAAGTATTGAAAAAAAGATTATTTCCGCAGAAGATCCT
ATAGAATATAAAATTCCTTTAGTGCAGCAAATTTTGCTCAATTCAAAAGTTGGAGTCGAGTTTAATAGTGTTTTAAGAGC
AATTTTAAGGCAAGATCCTGATATTATCATGATAGGAGAAATTCGCGATGAAGAAAGTTTAGATATTGCACTTAAAGCTT
CTTTAACAGGTCATTTGCTTTTAAGCACCTTGCATACTAATGATGCTCTTAGTACCATTGATAGGCTTTTGGATATGCAA
GCAAAAAGTTACTTGATAGCATCGGCTTTGAGTCTTGTTATAGCACAGCGTTTGGTTAGAAAACTTTGTCCTTGGTGTAA
GCAAAAAAGCAAAAAACATTATATTGAATTTGAAGGTGAATTTTTTGAACCAAAAGGATGTGAGCGTTGTCATCATAGTG
GATTTTTTGGCAGAGAATTAATCGCTGAGTGCTTAGAGATCAATGAAGATTTAGCTTGTGCAATTAGGGAAAATCAAGAT
AAAACGATTTTAATGGAGCTTGCAAAAAAATATGGCTTTCAAACTATGTTTGAACAAGGTTTAAAAAAAGCCAAAGAAGG
TCTTACAAGTATAGATGAGCTTTTAAGGGTTGTAAGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(157-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5T0G0P4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Jessica M Beauchamp et al. (2015) Characterization and localization of the Campylobacter jejuni transformation system proteins CtsE, CtsP, and CtsX. Journal of Bacteriology 197(3):636-45. [PMID: 25448813]
[2] Rebecca S Wiesner et al. (2003) Natural transformation of Campylobacter jejuni requires components of a type II secretion system. Journal of Bacteriology 185(18):5408-18. [PMID: 12949093]