Detailed information    

experimental Experimentally validated

Overview

Name   ctsX   Type   Machinery gene
Locus tag   CJJ81176_RS07055 Genome accession   NC_008787
Coordinates   1384795..1385382 (-) Length   195 a.a.
NCBI ID   WP_002851370.1    Uniprot ID   A0AB36G4D4
Organism   Campylobacter jejuni subsp. jejuni 81-176     
Function   DNA uptake   
DNA binding and uptake

Function

Given the transformation defect of strain RSW46, which lacks complete cj1472c, we gave this gene a cts designation and have termed it ctsX. Topological analysis of CtsX by the use of LacZ and PhoA fusions demonstrated it to be a bitopic, integral membrane protein with a cytoplasmic amino terminus and a periplasmic carboxyl terminus.


Genomic Context

Location: 1379795..1390382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJJ81176_RS07035 (CJJ81176_1460) - 1380543..1381306 (+) 764 Protein_1353 TIGR02757 family protein -
  CJJ81176_RS07040 (CJJ81176_1462) - 1381310..1382074 (+) 765 WP_002855301.1 TSUP family transporter -
  CJJ81176_RS07045 (CJJ81176_1463) ctsF 1382071..1383249 (-) 1179 WP_002869197.1 type II secretion system F family protein Machinery gene
  CJJ81176_RS07050 (CJJ81176_1464) ctsE 1383246..1384805 (-) 1560 WP_011812800.1 GspE/PulE family protein Machinery gene
  CJJ81176_RS07055 (CJJ81176_1465) ctsX 1384795..1385382 (-) 588 WP_002851370.1 membrane protein Machinery gene
  CJJ81176_RS07060 (CJJ81176_1466) ctsP 1385386..1385994 (-) 609 WP_011812801.1 ATP-binding protein Machinery gene
  CJJ81176_RS07065 (CJJ81176_1467) ctsD 1385987..1387399 (-) 1413 WP_002856543.1 pilus (MSHA type) biogenesis protein MshL Machinery gene
  CJJ81176_RS07070 (CJJ81176_1468) ctsR 1387377..1387688 (-) 312 WP_002855438.1 hypothetical protein Machinery gene

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 23359.43 Da        Isoelectric Point: 9.8409

>NTDB_id=1234 CJJ81176_RS07055 WP_002851370.1 1384795..1385382(-) (ctsX) [Campylobacter jejuni subsp. jejuni 81-176]
MQERIKELELRYKYFLLKKYLKYLLLIILISVIAFCFFVLMQKYNKQKNIYLQAIEHKKHLEQKILQAQILQEKNKISRE
KLYKELEEVKAVQENTHISKIEIDSKILNISDLKKSFYQNPSYEKALNLAKKYFDIKAYQKTIFWALKANELDKQKQDSW
LIFAQAKRALGEEKEAQSALDAYINYYGLMELDGK

Nucleotide


Download         Length: 588 bp        

>NTDB_id=1234 CJJ81176_RS07055 WP_002851370.1 1384795..1385382(-) (ctsX) [Campylobacter jejuni subsp. jejuni 81-176]
ATGCAAGAAAGAATTAAAGAGCTTGAGCTTAGGTATAAATACTTTTTACTTAAAAAATATTTAAAATATTTATTATTAAT
TATTCTAATATCAGTAATAGCATTTTGTTTTTTTGTGCTAATGCAAAAATATAATAAGCAAAAAAATATTTATTTGCAAG
CTATTGAACATAAAAAGCATTTGGAGCAAAAGATTCTTCAAGCGCAAATTTTACAAGAAAAAAATAAGATTTCTAGGGAA
AAGTTATATAAAGAACTAGAAGAAGTTAAAGCGGTGCAGGAAAATACACATATTAGCAAGATAGAAATTGATTCTAAAAT
TTTAAATATTTCTGATTTAAAAAAATCTTTTTATCAAAATCCAAGCTATGAAAAGGCATTAAATTTAGCTAAGAAGTATT
TTGATATTAAAGCTTATCAAAAGACAATATTTTGGGCTTTAAAAGCCAATGAACTTGACAAGCAAAAACAAGATTCTTGG
CTGATTTTTGCTCAGGCTAAAAGAGCTTTAGGCGAAGAAAAAGAAGCTCAAAGTGCTTTAGATGCTTATATAAATTATTA
TGGTTTAATGGAATTAGATGGAAAGTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Jessica M Beauchamp et al. (2015) Characterization and localization of the Campylobacter jejuni transformation system proteins CtsE, CtsP, and CtsX. Journal of Bacteriology 197(3):636-45. [PMID: 25448813]
[2] Rebecca S Wiesner et al. (2003) Natural transformation of Campylobacter jejuni requires components of a type II secretion system. Journal of Bacteriology 185(18):5408-18. [PMID: 12949093]