Detailed information    

experimental Experimentally validated

Overview

Name   ctsF   Type   Machinery gene
Locus tag   CJJ81176_RS07045 Genome accession   NC_008787
Coordinates   1382071..1383249 (-) Length   392 a.a.
NCBI ID   WP_002869197.1    Uniprot ID   Q7WYI6
Organism   Campylobacter jejuni subsp. jejuni 81-176     
Function   type II secretion system/type IV pilin; DNA uptake   
DNA binding and uptake

Function

Mutants of C. jejuni lacking the PSTC proteins CtsD, CtsE, CtsF, and CtsG decrease DNA uptake to background levels.


Genomic Context

Location: 1377071..1388249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJJ81176_RS07015 (CJJ81176_1456) - 1377612..1377953 (+) 342 WP_002869194.1 hypothetical protein -
  CJJ81176_RS07020 (CJJ81176_1457) flgM 1378016..1378213 (+) 198 WP_002869195.1 flagellar biosynthesis anti-sigma factor FlgM -
  CJJ81176_RS07025 (CJJ81176_1458) - 1378256..1378690 (+) 435 WP_002779263.1 flagellar protein FlgN -
  CJJ81176_RS07030 (CJJ81176_1459) flgK 1378700..1380526 (+) 1827 WP_002869196.1 flagellar hook-associated protein FlgK -
  CJJ81176_RS07035 (CJJ81176_1460) - 1380543..1381306 (+) 764 Protein_1353 TIGR02757 family protein -
  CJJ81176_RS07040 (CJJ81176_1462) - 1381310..1382074 (+) 765 WP_002855301.1 TSUP family transporter -
  CJJ81176_RS07045 (CJJ81176_1463) ctsF 1382071..1383249 (-) 1179 WP_002869197.1 type II secretion system F family protein Machinery gene
  CJJ81176_RS07050 (CJJ81176_1464) ctsE 1383246..1384805 (-) 1560 WP_011812800.1 GspE/PulE family protein Machinery gene
  CJJ81176_RS07055 (CJJ81176_1465) ctsX 1384795..1385382 (-) 588 WP_002851370.1 membrane protein Machinery gene
  CJJ81176_RS07060 (CJJ81176_1466) ctsP 1385386..1385994 (-) 609 WP_011812801.1 ATP-binding protein Machinery gene
  CJJ81176_RS07065 (CJJ81176_1467) ctsD 1385987..1387399 (-) 1413 WP_002856543.1 pilus (MSHA type) biogenesis protein MshL Machinery gene
  CJJ81176_RS07070 (CJJ81176_1468) ctsR 1387377..1387688 (-) 312 WP_002855438.1 hypothetical protein Machinery gene

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 45698.29 Da        Isoelectric Point: 9.0938

>NTDB_id=1228 CJJ81176_RS07045 WP_002869197.1 1382071..1383249(-) (ctsF) [Campylobacter jejuni subsp. jejuni 81-176]
MKFYEVEFLKNNQNYTKTIKAENLNTAQAKALSKNWKIIDIKEIQKSNFQRLKDENFILFFKELALLCEVGLSVQEAIRE
LYFMHSCKIMKKILDNLILAQNLNQAFENANFGLNRAELALIKTAEKTGKISEVFSQISKLREKSLESQKQLKKAFRYPT
LVFLSIIGAFLFLMLFVVPNFKDLFENLGVSLPFITHVMLEIYNFLDDYGIFCIFLFVIFIVMLILAYKNFHSFAFSCDF
LFLKVPLISRLIIYNQNYYFFMVFSLLLKNGISISKAFDLAIIGLENKFLIFQYKKLFSFIDSGLELSQAFKKIDIFDSL
VFSMLSVAMKSGRLEVLSEEIAKYYQQKSENLMDRFLVFLEPMMTLFVAFLVLFLALGIFLPMWELSSGVNF

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=1228 CJJ81176_RS07045 WP_002869197.1 1382071..1383249(-) (ctsF) [Campylobacter jejuni subsp. jejuni 81-176]
ATGAAATTTTATGAAGTAGAATTTCTTAAAAACAATCAAAATTATACAAAAACTATAAAAGCTGAAAATTTAAATACAGC
ACAAGCAAAAGCCTTATCAAAGAATTGGAAAATCATTGATATAAAAGAAATTCAAAAATCTAATTTTCAGAGATTAAAGG
ATGAAAATTTTATACTCTTTTTTAAAGAGCTAGCTTTGTTGTGTGAAGTTGGATTAAGTGTTCAAGAAGCGATTAGAGAA
TTATATTTTATGCATTCTTGCAAGATTATGAAAAAAATATTAGACAATTTAATTTTAGCCCAAAATCTAAATCAAGCTTT
TGAAAATGCTAATTTTGGATTAAATCGTGCGGAGTTAGCTTTGATTAAAACGGCTGAAAAGACAGGAAAAATCAGTGAAG
TGTTTAGTCAAATTTCTAAACTTCGAGAAAAAAGCTTAGAGAGTCAAAAACAATTAAAAAAGGCTTTTAGATATCCTACT
TTGGTTTTTTTAAGTATTATCGGAGCATTTTTGTTTTTAATGCTTTTTGTAGTGCCTAATTTTAAAGATTTATTTGAAAA
TTTAGGCGTGAGTTTGCCTTTTATCACGCATGTAATGTTAGAAATTTATAATTTTTTAGATGACTATGGAATTTTTTGCA
TATTTTTGTTTGTTATTTTTATTGTTATGCTTATTTTAGCTTATAAAAATTTTCATTCTTTTGCTTTTTCTTGTGATTTT
TTATTTTTAAAAGTACCTTTGATTTCTCGTTTGATTATTTATAATCAAAATTATTATTTTTTTATGGTTTTTTCTTTGCT
TTTAAAAAATGGAATTTCAATATCTAAAGCTTTTGATTTAGCTATTATAGGGCTTGAAAATAAATTTTTAATTTTTCAGT
ATAAAAAACTTTTTAGTTTTATAGATTCTGGTCTTGAGTTAAGTCAGGCTTTTAAAAAGATTGATATTTTTGATTCTTTG
GTATTTTCTATGCTCAGTGTTGCGATGAAAAGTGGAAGACTTGAAGTGTTAAGTGAAGAAATTGCCAAATATTATCAACA
AAAAAGTGAAAATTTAATGGATAGATTTTTGGTTTTTTTAGAGCCTATGATGACTTTATTTGTGGCGTTTTTAGTTTTGT
TTTTGGCTTTAGGTATATTTTTACCTATGTGGGAGCTTAGTTCGGGGGTCAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(261-382)

Type II secretion system (T2SS), protein F (T2SSF)

(60-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7WYI6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Rebecca S Wiesner et al. (2003) Natural transformation of Campylobacter jejuni requires components of a type II secretion system. Journal of Bacteriology 185(18):5408-18. [PMID: 12949093]