6098

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) /RES-Xre
Location 766495..767427 Replicon plasmid pSymB
Accession NC_003078
Organism Sinorhizobium meliloti 1021

Toxin (Protein)


Gene name - Uniprot ID Q92VJ4
Locus tag SM_RS20630 Protein ID WP_010975576.1
Coordinates 766873..767427 (+) Length 185 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID H0G7T7
Locus tag SM_RS20625 Protein ID WP_003534594.1
Coordinates 766495..766866 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SM_RS20605 (SM_b21123) 762382..762765 + 384 WP_010975573.1 RidA family protein -
SM_RS20610 (SM_b21124) 762782..764770 + 1989 WP_010975574.1 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha -
SM_RS20615 (SM_b21125) 764767..765636 + 870 WP_010975575.1 hydroxymethylglutaryl-CoA lyase -
SM_RS20620 (SM_b21126) 765633..766418 + 786 WP_003534600.1 crotonase/enoyl-CoA hydratase family protein -
SM_RS20625 (SM_b21127) 766495..766866 + 372 WP_003534594.1 DUF2384 domain-containing protein Antitoxin
SM_RS20630 (SM_b21128) 766873..767427 + 555 WP_010975576.1 RES family NAD+ phosphorylase Toxin
SM_RS20635 (SM_b21129) 767563..768932 - 1370 Protein_706 FAD-binding oxidoreductase -
SM_RS20640 (SM_b21130) 769044..770147 - 1104 WP_010975578.1 sulfate/molybdate ABC transporter ATP-binding protein -
SM_RS20645 (SM_b21131) 770163..771038 - 876 WP_003537156.1 sulfate ABC transporter permease subunit CysW -
SM_RS20650 (SM_b21132) 771028..771894 - 867 WP_010975580.1 sulfate ABC transporter permease subunit CysT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid - htpB 1..1683333 1683333


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(23-177)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(71-120)


Sequences

Toxin        


Download         Length: 185 a.a.        Molecular weight: 20459.35 Da        Isoelectric Point: 6.4901

>T6098 WP_010975576.1 NC_003078:766873-767427 [Sinorhizobium meliloti 1021]
MRKHIPPASRPDPEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Download         Length: 555 bp

>T6098 NC_003078:766873-767427 [Sinorhizobium meliloti 1021]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATCCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGGA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTGCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13113.26 Da        Isoelectric Point: 9.9996

>AT6098 WP_003534594.1 NC_003078:766495-766866 [Sinorhizobium meliloti 1021]
MMAVDFQIPAARFGDDHSPFLSARLVADRLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Download         Length: 372 bp

>AT6098 NC_003078:766495-766866 [Sinorhizobium meliloti 1021]
ATGATGGCCGTTGATTTCCAGATACCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACCGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T307 Pseudomonas putida KT2440

27.778

100

0.31

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92VJ4

Antitoxin

Source ID Structure
AlphaFold DB H0G7T7

References

(1) Branislava Milunovic et al. (2014) Cell growth inhibition upon deletion of four toxin-antitoxin loci from the megaplasmids of Sinorhizobium meliloti. Journal of Bacteriology 196(4):811-24. [PubMed:24317400] experimental


experimental experimental literature