Detailed information of TA system TA048461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1244932..1245599
• Replicon: plasmid pSymB
• Accession: NZ_CP009146
• Organism: Sinorhizobium meliloti strain RMO17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DU99_RS30035
• Protein ID: WP_017273388.1
• Coordinates: 1245156..1245599 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92WE1
• Locus tag: DU99_RS30030
• Protein ID: WP_010975287.1
• Coordinates: 1244932..1245159 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU99_RS30010	1240325..1241206	-	882	WP_003525869.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
DU99_RS30015	1241324..1242616	-	1293	WP_010975290.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
DU99_RS30020	1242985..1244274	+	1290	WP_017273387.1	SDR family oxidoreductase	-
DU99_RS30025	1244274..1244747	+	474	WP_015008545.1	DUF2269 family protein	-
DU99_RS30030	1244932..1245159	+	228	WP_010975287.1	hypothetical protein	Antitoxin
DU99_RS30035	1245156..1245599	+	444	WP_017273388.1	PIN domain-containing protein	Toxin
DU99_RS30040	1245615..1246553	-	939	WP_040121074.1	choline ABC transporter substrate-binding protein	-
DU99_RS30045	1246622..1247452	-	831	WP_014527489.1	SDR family oxidoreductase	-
DU99_RS30050	1247449..1247946	-	498	WP_010975282.1	acetone carboxylase subunit gamma	-
DU99_RS30055	1247950..1250142	-	2193	WP_040121075.1	hydantoinase B/oxoprolinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_003078 Sinorhizobium meliloti 1021 Mobilizable plasmid TA006098, TA020656 1..1683333 0.01	inside	Mobilizable plasmid	-	htpB	1..1617037	1617037

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15619.98 Da
• Isoelectric Point: 7.4215

>T48461 WP_017273388.1 NZ_CP009146:1245156-1245599 [Sinorhizobium meliloti]
VTFLLDVNVLIALIDPSHIGHDDAHEWFASIGQTAWATCPITENGVIRIVGNPKYPNSPGSPLPVMEIVAKLRSLPGHVF
WPDDVSLVGSSDIIPSKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTKGNSALHLIATNRS

Nucleotide

• Length: 444 bp

>T48461 NZ_CP009146:1245156-1245599 [Sinorhizobium meliloti]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCGCGTGGGCCACCTGCCCAATTACCGAAAATGGCGTCATCCGGATCGTCGGCAATCCCA
AATATCCCAATTCTCCCGGCTCACCGTTACCCGTGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8174.39 Da
• Isoelectric Point: 9.1096

>AT48461 WP_010975287.1 NZ_CP009146:1244932-1245159 [Sinorhizobium meliloti]
MRTTLAIDDDVLIAAKAMATQQQRSVGEVISELARRSLRRPRSGGERNGIPLLSPRPDAPPVTLEIVNALRDELA

Nucleotide

• Length: 228 bp

>AT48461 NZ_CP009146:1244932-1245159 [Sinorhizobium meliloti]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCACGTCCGGACGCACCGCCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q92WE1