Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-Xre
Location 338178..339110 Replicon plasmid psymB
Accession NZ_CP021828
Organism Sinorhizobium meliloti strain KH35c

Toxin (Protein)


Gene name - Uniprot ID Q92VJ4
Locus tag CDO23_RS27320 Protein ID WP_010975576.1
Coordinates 338556..339110 (+) Length 185 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID H0G7T7
Locus tag CDO23_RS27315 Protein ID WP_003534594.1
Coordinates 338178..338549 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO23_RS27295 334065..334448 + 384 WP_010975573.1 RidA family protein -
CDO23_RS27300 334465..336453 + 1989 WP_027993620.1 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha -
CDO23_RS27305 336450..337319 + 870 WP_003534602.1 hydroxymethylglutaryl-CoA lyase -
CDO23_RS27310 337316..338101 + 786 WP_027993619.1 crotonase/enoyl-CoA hydratase family protein -
CDO23_RS27315 338178..338549 + 372 WP_003534594.1 DUF2384 domain-containing protein Antitoxin
CDO23_RS27320 338556..339110 + 555 WP_010975576.1 RES family NAD+ phosphorylase Toxin
CDO23_RS27325 339246..340616 - 1371 WP_088218333.1 FAD-binding oxidoreductase -
CDO23_RS27330 340728..341831 - 1104 WP_010975578.1 sulfate/molybdate ABC transporter ATP-binding protein -
CDO23_RS27335 341847..342722 - 876 WP_003537156.1 sulfate ABC transporter permease subunit CysW -
CDO23_RS27340 342712..343578 - 867 WP_027993616.1 sulfate ABC transporter permease subunit CysT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid - htpB 1..1624370 1624370


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(23-177)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(71-120)


Sequences

Toxin        


Download         Length: 185 a.a.        Molecular weight: 20459.35 Da        Isoelectric Point: 6.4901

>T77548 WP_010975576.1 NZ_CP021828:338556-339110 [Sinorhizobium meliloti]
MRKHIPPASRPDPEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWRWN
GAEEPRLDVIDPDGRLPKSPASWV

Download         Length: 555 bp

>T77548 NZ_CP021828:338556-339110 [Sinorhizobium meliloti]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATCCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTGCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGCGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAAATCGCCCGCCTCATGGGTTTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13113.26 Da        Isoelectric Point: 9.9996

>AT77548 WP_003534594.1 NZ_CP021828:338178-338549 [Sinorhizobium meliloti]
MMAVDFQIPAARFGDDHSPFLSARLVADRLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Download         Length: 372 bp

>AT77548 NZ_CP021828:338178-338549 [Sinorhizobium meliloti]
ATGATGGCCGTTGATTTCCAGATACCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACCGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92VJ4

Antitoxin

Source ID Structure
AlphaFold DB H0G7T7

References