77532

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RHH(antitoxin)
Location 1437878..1438412 Replicon plasmid psymB
Accession NZ_CP021823
Organism Sinorhizobium meliloti strain KH46

Toxin (Protein)


Gene name parE Uniprot ID Q92X99
Locus tag CDO24_RS25210 Protein ID WP_010974986.1
Coordinates 1438116..1438412 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID H0FXK0
Locus tag CDO24_RS25205 Protein ID WP_003527850.1
Coordinates 1437878..1438126 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO24_RS25175 1433330..1434415 + 1086 WP_088199920.1 ABC transporter substrate-binding protein -
CDO24_RS25180 1434412..1435455 + 1044 WP_010974980.1 iron ABC transporter permease -
CDO24_RS25185 1435452..1436252 + 801 WP_010974981.1 ABC transporter ATP-binding protein -
CDO24_RS25190 1436256..1437050 + 795 WP_013850206.1 methyltransferase domain-containing protein -
CDO24_RS35470 1437134..1437259 - 126 Protein_1287 IS481 family transposase -
CDO24_RS25200 1437615..1437770 + 156 WP_010974985.1 hypothetical protein -
CDO24_RS25205 1437878..1438126 + 249 WP_003527850.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
CDO24_RS25210 1438116..1438412 + 297 WP_010974986.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CDO24_RS35105 1438493..1438753 - 261 Protein_1291 helix-turn-helix domain containing protein -
CDO24_RS25220 1439333..1439731 + 399 WP_010974988.1 hypothetical protein -
CDO24_RS25225 1439898..1440083 + 186 WP_010974989.1 hypothetical protein -
CDO24_RS25230 1440087..1440533 - 447 WP_014527651.1 DNA-binding protein -
CDO24_RS25235 1440823..1441032 + 210 WP_003527838.1 dodecin family protein -
CDO24_RS25240 1441566..1442969 + 1404 WP_088199922.1 sodium:alanine symporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid - htpB 1..1683883 1683883
- flank IS/Tn - - 1437134..1437325 191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-91)

Antitoxin

Antitoxin ParD

(3-78)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11473.15 Da        Isoelectric Point: 10.6593

>T77532 WP_010974986.1 NZ_CP021823:1438116-1438412 [Sinorhizobium meliloti]
MATERPFRLAPTAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAVGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Download         Length: 297 bp

>T77532 NZ_CP021823:1438116-1438412 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGTAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8888.73 Da        Isoelectric Point: 4.2176

>AT77532 WP_003527850.1 NZ_CP021823:1437878-1438126 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Download         Length: 249 bp

>AT77532 NZ_CP021823:1437878-1438126 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CCTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92X99

Antitoxin

Source ID Structure
AlphaFold DB H0FXK0

References