Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | /COG5642-COG5654 |
| Location | 2780224..2781137 | Replicon | chromosome |
| Accession | NC_002947 | ||
| Organism | Pseudomonas putida KT2440 | ||
Toxin (Protein)
| Gene name | - | Uniprot ID | Q88K57 |
| Locus tag | PP_2434 | Protein ID | 26989157 |
| Coordinates | 2780703..2781140 (+) | Length | 146 a.a. |
Antitoxin (Protein)
| Gene name | - | Uniprot ID | Q88K58 |
| Locus tag | PP_2433 | Protein ID | 26989156 |
| Coordinates | 2780257..2780706 (+) | Length | 150 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PP_RS12650 (PP_2428) | 2775681..2776880 | + | 1200 | WP_049587621.1 | sugar transporter | - |
| PP_RS12655 (PP_2429) | 2776920..2777504 | - | 585 | WP_010953382.1 | LysE family transporter | - |
| PP_RS12660 (PP_2430) | 2777555..2778385 | - | 831 | WP_003250518.1 | AraC family transcriptional regulator | - |
| PP_RS12665 (PP_2431) | 2778434..2779357 | - | 924 | WP_010953383.1 | LysR family transcriptional regulator | - |
| PP_RS12670 (PP_2432) | 2779461..2780114 | + | 654 | WP_010953384.1 | oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB | - |
| PP_RS12675 (PP_2433) | 2780257..2780706 | + | 450 | WP_003250525.1 | DUF2384 domain-containing protein | Antitoxin |
| PP_RS12680 (PP_2434) | 2780703..2781140 | + | 438 | WP_003250527.1 | RES family NAD+ phosphorylase | Toxin |
| PP_RS12685 (PP_2435) | 2781148..2782302 | - | 1155 | WP_049587669.1 | aminotransferase class V-fold PLP-dependent enzyme | - |
| PP_RS12690 (PP_2436) | 2782496..2783422 | - | 927 | WP_010953387.1 | LysR family transcriptional regulator | - |
| PP_RS12695 (PP_2437) | 2783563..2784792 | + | 1230 | WP_049587623.1 | acyl-CoA dehydrogenase | - |
| PP_RS12700 (PP_5522) | 2784956..2785204 | + | 249 | Protein_2471 | PAAR domain-containing protein | - |
| PP_RS12705 (PP_5523) | 2785207..2785596 | + | 390 | WP_049587628.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 146 a.a. Molecular weight: 15990.29 Da Isoelectric Point: 5.6007
>T307 26989157 NC_002947:2780703-2781140 [Pseudomonas putida KT2440]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAQPELTRTLGNRFLDDCSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAQPELTRTLGNRFLDDCSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF
Download Length: 438 bp
>T307 NC_002947:2780703-2781140 [Pseudomonas putida KT2440]
GTGATTTTGTGGCGAATCAGCGCCTATGCAGACTTGAGCGGTACGGGGGGCTTGCGTGTAAGTGGCCGCTGGCACCAGGC
GGGTCGGCCGGTGGTGTATGCCGCCACCAGCCCTCCTGGGGCGATGCTGGAAGTGTTGGTGCACCTGGAGATCGACCCCG
AGGATTTCCCTACCACCATGCGCTTGCTTCGCATCGAACTGCCCGACACGGTTTCCCAGGCGCAGTTGCCCGCTTTGCAG
CCCGGCTGGTCTGCTCAGCCTGAGTTGACCCGAACGCTGGGCAATCGCTTTCTGGATGACTGTTCGGCATTGCTGCTGCC
GGTGCCAAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATCC
AGGTTGAGGATTTCACGCCGGACAGCCGCCTGTTCTAG
GTGATTTTGTGGCGAATCAGCGCCTATGCAGACTTGAGCGGTACGGGGGGCTTGCGTGTAAGTGGCCGCTGGCACCAGGC
GGGTCGGCCGGTGGTGTATGCCGCCACCAGCCCTCCTGGGGCGATGCTGGAAGTGTTGGTGCACCTGGAGATCGACCCCG
AGGATTTCCCTACCACCATGCGCTTGCTTCGCATCGAACTGCCCGACACGGTTTCCCAGGCGCAGTTGCCCGCTTTGCAG
CCCGGCTGGTCTGCTCAGCCTGAGTTGACCCGAACGCTGGGCAATCGCTTTCTGGATGACTGTTCGGCATTGCTGCTGCC
GGTGCCAAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATCC
AGGTTGAGGATTTCACGCCGGACAGCCGCCTGTTCTAG
Antitoxin
Download Length: 150 a.a. Molecular weight: 16956.46 Da Isoelectric Point: 5.7258
>AT307 26989156 NC_002947:2780257-2780706 [Pseudomonas putida KT2440]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL
Download Length: 450 bp
>AT307 NC_002947:2780257-2780706 [Pseudomonas putida KT2440]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCT
TGCCAGCGATTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCACCTGGTTGGTAAAGCTGACCGAACGCGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATCCCGCTGCGCACCCTCAAATCCCGTATCGAGCGTGACCAACCGCTGACC
GTGGATGAGAGCGATCGGCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAGGCCGTGTTCGGTGAAGCGGGCAAGGC
CAAACGCTGGCTTTCCAAGCCCAAGGAACGCTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAAGGCACCA
CGCAGGTCGAAGAAATGCTGCTGCAGATTGCCGAGGGCTATGGCCTGTGA
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGACATTCCCGAGCT
TGCCAGCGATTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCACCTGGTTGGTAAAGCTGACCGAACGCGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATCCCGCTGCGCACCCTCAAATCCCGTATCGAGCGTGACCAACCGCTGACC
GTGGATGAGAGCGATCGGCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAGGCCGTGTTCGGTGAAGCGGGCAAGGC
CAAACGCTGGCTTTCCAAGCCCAAGGAACGCTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAAGGCACCA
CGCAGGTCGAAGAAATGCTGCTGCAGATTGCCGAGGGCTATGGCCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10044 | Pseudomonas resinovorans NBRC 106553 |
84.828 |
100 |
0.848 |
| T10145 | Sphingobium sp. YBL2 |
36.538 |
100 |
0.393 |
| T1222 | Photorhabdus luminescens subsp. laumondii TTO1 |
36.601 |
100 |
0.386 |
| T3484 | Yersinia enterocolitica subsp. enterocolitica 8081 |
34.043 |
97.241 |
0.331 |
| T6098 | Sinorhizobium meliloti 1021 |
27.778 |
100 |
0.31 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10044 | Pseudomonas resinovorans NBRC 106553 |
83.221 |
100 |
0.832 |
Multiple sequence alignment
References
(1) Ragnhild Bager Skjerning et al. (2019) The RES domain toxins of RES-Xre toxin-antitoxin modules induce cell stasis by degrading NAD+. Molecular Microbiology 111(1):221-236. [PubMed:30315706]