Detailed information of TA system TA010145

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): ParST/-
• Location: 3826190..3827151
• Replicon: chromosome
• Accession: NZ_CP010954
• Organism: Sphingobium sp. YBL2

Toxin (Protein)

• Gene name: ParT
• Uniprot ID: A0A5B8CC27
• Locus tag: TZ53_RS17650
• Protein ID: WP_044662136.1
• Coordinates: 3826190..3826675 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ParS
• Uniprot ID: A0A197BZ03
• Locus tag: TZ53_RS17655
• Protein ID: WP_044662137.1
• Coordinates: 3826672..3827151 (-)
• Length: 160 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TZ53_RS17625 (TZ53_17635)	3821658..3822077	+	420	WP_044662131.1	hypothetical protein	-
TZ53_RS17630 (TZ53_17640)	3822315..3822734	+	420	WP_044662132.1	helix-turn-helix domain-containing protein	-
TZ53_RS17635 (TZ53_17645)	3822744..3823376	+	633	WP_044662133.1	GntR family transcriptional regulator	-
TZ53_RS26880	3823500..3823634	+	135	WP_137709742.1	benenodin family lasso peptide	-
TZ53_RS17640 (TZ53_17650)	3823700..3824356	+	657	WP_082718614.1	lasso peptide biosynthesis B2 protein	-
TZ53_RS17645 (TZ53_17655)	3824356..3826113	+	1758	WP_044662135.1	asparagine synthase C-terminal domain-containing protein	-
TZ53_RS17650 (TZ53_17660)	3826190..3826675	-	486	WP_044662136.1	RES family NAD+ phosphorylase	Toxin
TZ53_RS17655 (TZ53_17665)	3826672..3827151	-	480	WP_044662137.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
TZ53_RS17660 (TZ53_17670)	3827239..3829320	-	2082	WP_044662138.1	Atxe2 family lasso peptide isopeptidase	-
TZ53_RS17665 (TZ53_17675)	3829320..3831884	-	2565	WP_052628225.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3793317..4001136	207819

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (5-149)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17375.85 Da
• Isoelectric Point: 7.1664

>T10145 WP_044662136.1 NZ_CP010954:c3826675-3826190 [Sphingobium sp. YBL2]
VTTSFWRIATDARTYEADDLSGAGAKITGGRWNEVGVAIVYAASSRALACLETVVHLNSGGLPLNRYLVEIEVPDEVLAS
AEVATPGNLPVGWDAEPAGRVSISFGSQWAQSQRTALLLVPSVIVPEETNLLINPAHPDAKGIKARKVRKWLYDPRMIRK
A

Nucleotide

• Length: 486 bp

>T10145 NZ_CP010954:c3826675-3826190 [Sphingobium sp. YBL2]
GTGACGACAAGCTTTTGGAGAATCGCAACCGACGCCAGGACCTATGAAGCGGACGACCTGTCAGGCGCCGGCGCCAAGAT
CACCGGGGGCCGGTGGAATGAGGTCGGCGTCGCCATCGTCTATGCGGCGAGCAGCCGGGCACTCGCCTGCCTGGAGACGG
TCGTTCATCTGAACTCGGGTGGGTTGCCGCTCAATCGCTACCTCGTCGAAATCGAGGTGCCGGACGAGGTGCTGGCATCA
GCGGAGGTCGCGACACCCGGCAATCTCCCGGTCGGCTGGGATGCCGAACCGGCGGGACGGGTCAGCATCTCATTCGGATC
GCAATGGGCGCAGAGCCAAAGGACAGCGCTGCTGCTCGTCCCTTCCGTCATTGTGCCAGAGGAGACTAATCTCCTCATCA
ATCCCGCGCACCCCGATGCCAAGGGGATCAAGGCCCGGAAGGTGCGCAAATGGCTCTATGATCCAAGGATGATCCGCAAG
GCGTGA

Antitoxin

Protein

• Length: 160 a.a.
• Molecular weight: 17037.46 Da
• Isoelectric Point: 5.2707

>AT10145 WP_044662137.1 NZ_CP010954:c3827151-3826672 [Sphingobium sp. YBL2]
MSELETGARKAQGKGTPPFAYKALYRASPVDRIGVIKKGVAASDLKRFIAALHVDQKVMFDALNLKTATVNKKAANNQPL
STEDSERVLGLAKLVGQLEDMVEESGETDGFDAPEWLSSWLRQPLPALGGVNPIDLLDTMEGQAVVSRALAQIQSGAFA

Nucleotide

• Length: 480 bp

>AT10145 NZ_CP010954:c3827151-3826672 [Sphingobium sp. YBL2]
ATGTCCGAACTCGAAACTGGCGCGCGCAAGGCGCAGGGGAAAGGCACGCCCCCCTTTGCCTATAAGGCGCTGTACCGTGC
CTCGCCGGTCGATCGAATCGGCGTCATCAAGAAGGGCGTGGCGGCCAGCGACCTGAAGCGCTTCATCGCCGCCTTGCATG
TGGACCAGAAGGTCATGTTCGACGCGCTCAATCTCAAGACGGCCACGGTGAACAAGAAGGCGGCGAACAACCAGCCGCTC
TCGACGGAAGACAGTGAACGGGTTCTGGGCCTTGCAAAACTGGTCGGCCAGCTCGAAGACATGGTCGAGGAATCGGGCGA
GACGGATGGGTTCGATGCGCCCGAATGGCTCTCGTCCTGGCTGCGTCAGCCTCTCCCGGCGCTGGGAGGCGTCAATCCGA
TCGATCTTCTCGACACGATGGAGGGACAGGCCGTCGTGTCCCGCGCCCTTGCGCAAATCCAGAGCGGCGCCTTTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T307	Pseudomonas putida KT2440	36.538	100	0.393
T10044	Pseudomonas resinovorans NBRC 106553	39.568	95.862	0.379
T3484	Yersinia enterocolitica subsp. enterocolitica 8081	36.301	96.053	0.349
T1222	Photorhabdus luminescens subsp. laumondii TTO1	35	89.744	0.314

Multiple sequence alignment

Structures

Toxin

• PDB: 6D0H
• PDB: 6D0I

Antitoxin

• AlphaFold DB: A0A197BZ03

References

(1) Frank J Piscotta et al. (2019) ParST is a widespread toxin-antitoxin module that targets nucleotide metabolism. Proceedings of The National Academy of Sciences of The United States of America 116(3):826-834. [PubMed:30598453]


 experimental literature