Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) prcAT/-
Location 184572..185455 Replicon plasmid pCAR1.3
Accession NC_021506
Organism Pseudomonas resinovorans NBRC 106553

Toxin (Protein)


Gene name PrcT Uniprot ID S6AYH8
Locus tag PCA10_RS29090 Protein ID WP_011078034.1
Coordinates 185018..185455 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name PrcA Uniprot ID S6AKX1
Locus tag PCA10_RS29085 Protein ID WP_011078033.1
Coordinates 184572..185021 (+) Length 150 a.a.

Genomic Context

PCA10_RS29065PCA10_RS29070PCA10_RS29075PCA10_RS29085PCA10_RS29090PCA10_RS29095PCA10_RS29100PCA10_RS29105
Location: 180287..181945 (1659 bp)
Type: Others
Protein ID: WP_011078029.1
Location: 182113..183363 (1251 bp)
Type: Others
Protein ID: WP_011078030.1
Location: 183706..184149 (444 bp)
Type: Others
Protein ID: WP_011078031.1
Location: 184572..185021 (450 bp)
Type: Antitoxin
Protein ID: WP_011078033.1
Location: 185018..185455 (438 bp)
Type: Toxin
Protein ID: WP_011078034.1
Location: 185808..188822 (3015 bp)
Type: Others
Protein ID: WP_011078035.1
Location: 188819..189181 (363 bp)
Type: Others
Protein ID: WP_011078036.1
Location: 189207..190166 (960 bp)
Type: Others
Protein ID: WP_011078037.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
PCA10_RS29065 (PCA10_p2030)180287..181945+1659WP_011078029.1methyl-accepting chemotaxis protein-
PCA10_RS29070 (PCA10_p2040)182113..183363+1251WP_011078030.1IS256 family transposase-
PCA10_RS29075 (PCA10_p2060)183706..184149+444WP_011078031.1hypothetical protein-
PCA10_RS29085 (PCA10_p2080)184572..185021+450WP_011078033.1DUF2384 domain-containing proteinAntitoxin
PCA10_RS29090 (PCA10_p2090)185018..185455+438WP_011078034.1RES family NAD+ phosphorylaseToxin
PCA10_RS29095 (PCA10_p2100)185808..188822+3015WP_011078035.1Tn3 family transposase-
PCA10_RS29100 (PCA10_p2110)188819..189181+363WP_011078036.1hypothetical protein-
PCA10_RS29105 (PCA10_p2120)189207..190166-960WP_011078037.1efflux pump-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideNon-Mobilizable plasmid--1..198965198965
-insideIScluster/Tn--176518..18882212304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-134)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(97-146)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15867.09 Da        Isoelectric Point: 4.4777

>T10044 WP_011078034.1 NC_021506:185018-185455 [Pseudomonas resinovorans NBRC 106553]
VILWRISSYADLSGTGGLRVSGRWHQAGRPVVYTATSPPGAMLEVLVHLEIDPEDFPATMQLLRIEVPDSVSQAQMPTLP
AGWSAETELTRGIGNQFLDECNALLLPVPSAIIPSTVNYLFNPRHPEAQSATIQVELFTPDSRLF

Download         Length: 438 bp

>T10044 NC_021506:185018-185455 [Pseudomonas resinovorans NBRC 106553]
GTGATTCTGTGGCGGATCAGCTCATATGCTGACTTGAGCGGGACAGGCGGCTTGCGCGTGAGCGGCCGTTGGCACCAGGC
CGGCAGACCGGTCGTCTACACCGCGACCAGCCCGCCGGGAGCAATGCTCGAGGTGCTTGTTCATCTGGAAATTGATCCAG
AGGATTTTCCCGCGACGATGCAGCTTCTCCGTATCGAAGTGCCTGACTCTGTATCACAAGCCCAGATGCCCACTTTGCCG
GCTGGTTGGTCGGCAGAGACCGAGCTCACCAGAGGCATTGGTAACCAGTTCCTTGATGAGTGCAACGCACTGCTACTGCC
AGTGCCCAGCGCTATCATACCGAGCACGGTGAACTACCTGTTCAACCCTCGGCACCCAGAAGCACAGAGCGCGACTATTC
AGGTTGAGCTTTTCACCCCTGATTCGAGGTTGTTCTAG

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16963.45 Da        Isoelectric Point: 6.1074

>AT10044 WP_011078033.1 NC_021506:184572-185021 [Pseudomonas resinovorans NBRC 106553]
MLAEVLRDHGYSAYRARLQILLEIPESASDFEIHTRISHGFSAARLMRLTEEGVVTPLERDQIIPLRTLKNRIERDQPLT
VEESDRLFRSAHITAMAEAIFGDADKAKRWLSKPKERFSGLTPMQLLTTQQGTSQVEEMLLQIAEGFAL

Download         Length: 450 bp

>AT10044 NC_021506:184572-185021 [Pseudomonas resinovorans NBRC 106553]
ATGCTTGCTGAAGTCCTACGCGACCACGGGTATTCAGCTTACCGTGCGCGCTTACAAATACTGCTGGAGATCCCAGAGTC
TGCCAGCGACTTCGAGATTCACACCAGAATCAGCCATGGCTTTTCCGCAGCGCGGTTAATGAGGCTCACTGAAGAGGGTG
TGGTTACCCCGCTGGAGCGAGACCAGATTATCCCGCTGCGCACCCTGAAAAACCGGATCGAGCGCGATCAGCCCCTCACT
GTCGAGGAAAGCGACCGGCTCTTCCGATCGGCGCACATCACCGCGATGGCCGAGGCTATCTTTGGGGACGCCGACAAGGC
CAAGCGATGGCTCTCCAAGCCCAAGGAACGATTCTCTGGGTTGACGCCCATGCAACTGCTCACAACTCAGCAAGGCACAA
GCCAGGTTGAAGAGATGCTGCTGCAAATCGCCGAAGGCTTCGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T307Pseudomonas putida KT2440

84.828

100

0.848

T1222Photorhabdus luminescens subsp. laumondii TTO1

37.255

100

0.393

T10145Sphingobium sp. YBL2

39.568

95.862

0.379

T3484Yersinia enterocolitica subsp. enterocolitica 8081

34.307

94.483

0.324
Showing 1 to 4 of 4 rows

Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT307Pseudomonas putida KT2440

83.221

100

0.832
Showing 1 to 1 of 1 rows

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
AlphaFold DBS6AYH8

Antitoxin

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Source
ID
Structure
AlphaFold DBS6AKX1

References

(1) Aya Takashima et al. (2022) A toxin-antitoxin system confers stability to the IncP-7 plasmid pCAR1. Gene 812:146068. [PubMed:34838639] experimental


experimental experimental literature