Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | prcAT/- |
| Location | 184572..185455 | Replicon | plasmid pCAR1.3 |
| Accession | NC_021506 | ||
| Organism | Pseudomonas resinovorans NBRC 106553 | ||
Toxin (Protein)
| Gene name | PrcT | Uniprot ID | S6AYH8 |
| Locus tag | PCA10_RS29090 | Protein ID | WP_011078034.1 |
| Coordinates | 185018..185455 (+) | Length | 146 a.a. |
Antitoxin (Protein)
| Gene name | PrcA | Uniprot ID | S6AKX1 |
| Locus tag | PCA10_RS29085 | Protein ID | WP_011078033.1 |
| Coordinates | 184572..185021 (+) | Length | 150 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PCA10_RS29065 (PCA10_p2030) | 180287..181945 | + | 1659 | WP_011078029.1 | methyl-accepting chemotaxis protein | - |
| PCA10_RS29070 (PCA10_p2040) | 182113..183363 | + | 1251 | WP_011078030.1 | IS256 family transposase | - |
| PCA10_RS29075 (PCA10_p2060) | 183706..184149 | + | 444 | WP_011078031.1 | hypothetical protein | - |
| PCA10_RS29085 (PCA10_p2080) | 184572..185021 | + | 450 | WP_011078033.1 | DUF2384 domain-containing protein | Antitoxin |
| PCA10_RS29090 (PCA10_p2090) | 185018..185455 | + | 438 | WP_011078034.1 | RES family NAD+ phosphorylase | Toxin |
| PCA10_RS29095 (PCA10_p2100) | 185808..188822 | + | 3015 | WP_011078035.1 | Tn3 family transposase | - |
| PCA10_RS29100 (PCA10_p2110) | 188819..189181 | + | 363 | WP_011078036.1 | hypothetical protein | - |
| PCA10_RS29105 (PCA10_p2120) | 189207..190166 | - | 960 | WP_011078037.1 | efflux pump | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..198965 | 198965 | |
| - | inside | IScluster/Tn | - | - | 176518..188822 | 12304 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 146 a.a. Molecular weight: 15867.09 Da Isoelectric Point: 4.4777
>T10044 WP_011078034.1 NC_021506:185018-185455 [Pseudomonas resinovorans NBRC 106553]
VILWRISSYADLSGTGGLRVSGRWHQAGRPVVYTATSPPGAMLEVLVHLEIDPEDFPATMQLLRIEVPDSVSQAQMPTLP
AGWSAETELTRGIGNQFLDECNALLLPVPSAIIPSTVNYLFNPRHPEAQSATIQVELFTPDSRLF
VILWRISSYADLSGTGGLRVSGRWHQAGRPVVYTATSPPGAMLEVLVHLEIDPEDFPATMQLLRIEVPDSVSQAQMPTLP
AGWSAETELTRGIGNQFLDECNALLLPVPSAIIPSTVNYLFNPRHPEAQSATIQVELFTPDSRLF
Download Length: 438 bp
>T10044 NC_021506:185018-185455 [Pseudomonas resinovorans NBRC 106553]
GTGATTCTGTGGCGGATCAGCTCATATGCTGACTTGAGCGGGACAGGCGGCTTGCGCGTGAGCGGCCGTTGGCACCAGGC
CGGCAGACCGGTCGTCTACACCGCGACCAGCCCGCCGGGAGCAATGCTCGAGGTGCTTGTTCATCTGGAAATTGATCCAG
AGGATTTTCCCGCGACGATGCAGCTTCTCCGTATCGAAGTGCCTGACTCTGTATCACAAGCCCAGATGCCCACTTTGCCG
GCTGGTTGGTCGGCAGAGACCGAGCTCACCAGAGGCATTGGTAACCAGTTCCTTGATGAGTGCAACGCACTGCTACTGCC
AGTGCCCAGCGCTATCATACCGAGCACGGTGAACTACCTGTTCAACCCTCGGCACCCAGAAGCACAGAGCGCGACTATTC
AGGTTGAGCTTTTCACCCCTGATTCGAGGTTGTTCTAG
GTGATTCTGTGGCGGATCAGCTCATATGCTGACTTGAGCGGGACAGGCGGCTTGCGCGTGAGCGGCCGTTGGCACCAGGC
CGGCAGACCGGTCGTCTACACCGCGACCAGCCCGCCGGGAGCAATGCTCGAGGTGCTTGTTCATCTGGAAATTGATCCAG
AGGATTTTCCCGCGACGATGCAGCTTCTCCGTATCGAAGTGCCTGACTCTGTATCACAAGCCCAGATGCCCACTTTGCCG
GCTGGTTGGTCGGCAGAGACCGAGCTCACCAGAGGCATTGGTAACCAGTTCCTTGATGAGTGCAACGCACTGCTACTGCC
AGTGCCCAGCGCTATCATACCGAGCACGGTGAACTACCTGTTCAACCCTCGGCACCCAGAAGCACAGAGCGCGACTATTC
AGGTTGAGCTTTTCACCCCTGATTCGAGGTTGTTCTAG
Antitoxin
Download Length: 150 a.a. Molecular weight: 16963.45 Da Isoelectric Point: 6.1074
>AT10044 WP_011078033.1 NC_021506:184572-185021 [Pseudomonas resinovorans NBRC 106553]
MLAEVLRDHGYSAYRARLQILLEIPESASDFEIHTRISHGFSAARLMRLTEEGVVTPLERDQIIPLRTLKNRIERDQPLT
VEESDRLFRSAHITAMAEAIFGDADKAKRWLSKPKERFSGLTPMQLLTTQQGTSQVEEMLLQIAEGFAL
MLAEVLRDHGYSAYRARLQILLEIPESASDFEIHTRISHGFSAARLMRLTEEGVVTPLERDQIIPLRTLKNRIERDQPLT
VEESDRLFRSAHITAMAEAIFGDADKAKRWLSKPKERFSGLTPMQLLTTQQGTSQVEEMLLQIAEGFAL
Download Length: 450 bp
>AT10044 NC_021506:184572-185021 [Pseudomonas resinovorans NBRC 106553]
ATGCTTGCTGAAGTCCTACGCGACCACGGGTATTCAGCTTACCGTGCGCGCTTACAAATACTGCTGGAGATCCCAGAGTC
TGCCAGCGACTTCGAGATTCACACCAGAATCAGCCATGGCTTTTCCGCAGCGCGGTTAATGAGGCTCACTGAAGAGGGTG
TGGTTACCCCGCTGGAGCGAGACCAGATTATCCCGCTGCGCACCCTGAAAAACCGGATCGAGCGCGATCAGCCCCTCACT
GTCGAGGAAAGCGACCGGCTCTTCCGATCGGCGCACATCACCGCGATGGCCGAGGCTATCTTTGGGGACGCCGACAAGGC
CAAGCGATGGCTCTCCAAGCCCAAGGAACGATTCTCTGGGTTGACGCCCATGCAACTGCTCACAACTCAGCAAGGCACAA
GCCAGGTTGAAGAGATGCTGCTGCAAATCGCCGAAGGCTTCGCTCTGTGA
ATGCTTGCTGAAGTCCTACGCGACCACGGGTATTCAGCTTACCGTGCGCGCTTACAAATACTGCTGGAGATCCCAGAGTC
TGCCAGCGACTTCGAGATTCACACCAGAATCAGCCATGGCTTTTCCGCAGCGCGGTTAATGAGGCTCACTGAAGAGGGTG
TGGTTACCCCGCTGGAGCGAGACCAGATTATCCCGCTGCGCACCCTGAAAAACCGGATCGAGCGCGATCAGCCCCTCACT
GTCGAGGAAAGCGACCGGCTCTTCCGATCGGCGCACATCACCGCGATGGCCGAGGCTATCTTTGGGGACGCCGACAAGGC
CAAGCGATGGCTCTCCAAGCCCAAGGAACGATTCTCTGGGTTGACGCCCATGCAACTGCTCACAACTCAGCAAGGCACAA
GCCAGGTTGAAGAGATGCTGCTGCAAATCGCCGAAGGCTTCGCTCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T307 | Pseudomonas putida KT2440 |
84.828 |
100 |
0.848 |
| T1222 | Photorhabdus luminescens subsp. laumondii TTO1 |
37.255 |
100 |
0.393 |
| T10145 | Sphingobium sp. YBL2 |
39.568 |
95.862 |
0.379 |
| T3484 | Yersinia enterocolitica subsp. enterocolitica 8081 |
34.307 |
94.483 |
0.324 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT307 | Pseudomonas putida KT2440 |
83.221 |
100 |
0.832 |
Multiple sequence alignment
References
(1) Aya Takashima et al. (2022) A toxin-antitoxin system confers stability to the IncP-7 plasmid pCAR1. Gene 812:146068. [PubMed:34838639]