Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS17503  |
| T6SS type | Type i1 |
| Strain | Aeromonas hydrophila strain NF1 |
| Replicon (RefSeq) | chromosome (NZ_JDWB01000021) |
| Location | 42575..80489 |
| Function | anti-bacterial; anti-eukaryotic |
| Comment | NF1 T6SS Is Critical in NF and Peritonitis Mouse Models of Infection. An average 9% of NF2-lux survived after incubation for 4 h with NF1, while survival of NF2-lux increased on average of 31% and 63% when mixed with NF1ΔtseC and NF1ΔvasK, respectively. A significant difference in killing by NF1ΔtseC and NF1ΔvasK of NF2-lux suggested that other T6SS effectors also contribute to NF2 clearance. |
| References | [1] Fernández-Bravo A, Kilgore PB, Andersson JA, Blears E, Figueras MJ, Hasan NA, Colwell RR, Sha J, Chopra AK. et al (2019) T6SS and ExoA of flesh-eating Aeromonas hydrophila in peritonitis and necrotizing fasciitis during mono- and polymicrobial infections.. Proc Natl Acad Sci U S A. 116(48):24084-24092. [PudMed:31712444] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TagH | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM | VasH |
| Number | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| BE47_RS0109195 (trxB) | 37941..38891 | + | 951 | WP_029301170.1 | thioredoxin-disulfide reductase | ||
| BE47_RS0109200 | 38949..40196 | + | 1248 | WP_029301171.1 | response regulator | ||
| BE47_RS0109205 | 40307..40777 | - | 471 | WP_016350454.1 | glycine zipper 2TM domain-containing protein | ||
| BE47_RS0109210 (aat) | 40797..41504 | + | 708 | WP_029301172.1 | leucyl/phenylalanyl-tRNA--protein transferase | ||
| BE47_RS0109215 | 41501..42217 | + | 717 | WP_011705735.1 | arginyltransferase | ||
| BE47_RS0109220 (infA) | 42287..42505 | + | 219 | WP_005300033.1 | translation initiation factor IF-1 | ||
| T6CP259519 | TssH | BE47_RS0109225 (clpA) | 42575..44827 | - | 2253 | WP_024944043.1 | ATP-dependent Clp protease ATP-binding subunit ClpA |
| BE47_RS0109230 (clpS) | 44887..45204 | - | 318 | WP_005300028.1 | ATP-dependent Clp protease adapter ClpS | ||
| BE47_RS0109235 (cspD) | 45433..45651 | + | 219 | WP_005300025.1 | cold shock-like protein CspD | ||
| BE47_RS0109240 (folD) | 45759..46622 | - | 864 | WP_029301173.1 | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD | ||
| BE47_RS24695 | 47917..48618 | - | 702 | WP_155272126.1 | hypothetical protein | ||
| BE47_RS0109260 | 49445..51346 | - | 1902 | pseudogene | hypothetical protein | ||
| T6CP259520 | TssI | BE47_RS0109265 | 51381..53432 | - | 2052 | WP_029301175.1 | type VI secretion system tip protein VgrG |
| BE47_RS0109270 | 53443..53730 | - | 288 | WP_017411057.1 | type VI secretion system PAAR protein | ||
| T6CP259521 | TssA | BE47_RS0109275 | 53988..55418 | - | 1431 | WP_029301176.1 | type VI secretion system ImpA family N-terminal domain-containing protein |
| T6CP259522 | TssM | BE47_RS0109280 (tssM) | 55465..58950 | - | 3486 | WP_041213753.1 | type VI secretion system membrane subunit TssM |
| T6CP259523 | TssA | BE47_RS0109285 (tssA) | 58992..60437 | - | 1446 | WP_029301178.1 | type VI secretion system protein TssA |
| BE47_RS0109290 (tagO) | 60446..61051 | - | 606 | WP_029301179.1 | type VI secretion system-associated protein TagO | ||
| T6CP259525 | VasH | BE47_RS0109295 (vasH) | 61051..62589 | - | 1539 | WP_029301180.1 | sigma-54-dependent transcriptional regulator VasH |
| T6CP259526 | TssH | BE47_RS0109300 (tssH) | 62592..65234 | - | 2643 | WP_029301181.1 | type VI secretion system ATPase TssH |
| T6CP259527 | TssL | BE47_RS0109305 | 65255..66034 | - | 780 | WP_029301182.1 | type IVB secretion system protein IcmH/DotU |
| T6CP259528 | TssK | BE47_RS0109310 (tssK) | 66057..67391 | - | 1335 | WP_029301183.1 | type VI secretion system baseplate subunit TssK |
| T6CP259529 | TssJ | BE47_RS0109315 (tssJ) | 67394..67909 | - | 516 | WP_011705718.1 | type VI secretion system lipoprotein TssJ |
| T6CP259530 | TagH | BE47_RS0109320 (tagH) | 67909..69159 | - | 1251 | WP_029301184.1 | type VI secretion system-associated FHA domain protein TagH |
| T6CP259531 | TssG | BE47_RS0109325 (tssG) | 69216..70214 | - | 999 | WP_029301185.1 | type VI secretion system baseplate subunit TssG |
| T6CP259532 | TssF | BE47_RS0109330 (tssF) | 70178..71944 | - | 1767 | WP_029301186.1 | type VI secretion system baseplate subunit TssF |
| T6CP259533 | TssE | BE47_RS0109335 (tssE) | 71948..72379 | - | 432 | WP_029301187.1 | type VI secretion system baseplate subunit TssE |
| T6CP259534 | TssC | BE47_RS0109340 (tssC) | 72386..73864 | - | 1479 | WP_005299978.1 | type VI secretion system contractile sheath large subunit |
| T6CP259535 | TssB | BE47_RS0109345 (tssB) | 73903..74406 | - | 504 | WP_029301188.1 | type VI secretion system contractile sheath small subunit |
| BE47_RS0109350 | 75084..76466 | - | 1383 | WP_029301189.1 | SEL1-like repeat protein | ||
| BE47_RS24700 | 77444..77656 | - | 213 | pseudogene | transposase | ||
| BE47_RS0109355 (vgrG) | 77753..79693 | - | 1941 | pseudogene | type VI secretion system effector VgrG | ||
| T6CP259536 | TssD | BE47_RS0109360 (hcp1) | 79971..80489 | - | 519 | WP_010634822.1 | type VI secretion system effector Hcp1 |
| BE47_RS23970 | 81191..82310 | + | 1120 | pseudogene | IS3 family transposase | ||
| BE47_RS24705 | 82303..82527 | - | 225 | WP_155272127.1 | hypothetical protein | ||
| BE47_RS0109370 | 83080..83466 | - | 387 | WP_029301191.1 | hypothetical protein | ||
| BE47_RS25155 | 83802..84080 | - | 279 | WP_029301192.1 | immunity 26 domain-containing protein | ||
| BE47_RS24715 | 84222..84806 | - | 585 | WP_155272128.1 | hypothetical protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF76651 |
BE47_RS0112955 (tseC) | Tse | IMU38224 |
Aeromonas hydrophila strain NF1 | chromosome (NZ_JDWB01000029) | 59956..63138 (-) | WP_029301617.1 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU38224 |
BE47_RS23540 (tsiC) | EFF76651 |
Aeromonas hydrophila strain NF1 | chromosome (NZ_JDWB01000029) | 59225..59956 (-) | WP_050490294.1 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|