Detailed information of component protein
Summary
| Component ID | T6CP259527 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS17503  (Type i1) |
| Strain | Aeromonas hydrophila strain NF1 |
| Replicon | chromosome |
| Sequence | Protein sequence (260 a.a.); Nucleotide sequence (780 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BE47_RS0109305 |
| Coordinate (Strand) | 65255..66034 (-) |
| NCBI ID | WP_029301182.1 |
| RefSeq | NZ_JDWB01000021 |
| Uniprot ID | UPI0004937F51 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 9.2e-61 | 39..241 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-218 | MTTDIIKNEQLSDLLFDHAEQLDMDSDYWFRLRGQSINPMIDAVTPLLGLVQRVRLLSRYDRVPELYQRVVTEIQAIEQELMAQGYENGVVLSFRYILCTFIDEAVMGRDWGSQSEWSEHSLLTRFHNETWGGEKVFVLLARLQEDPVRYRDILEFIYLCLCLGFEGRYKVMSKGQGEFERIVRQLHKQLAPEAGGEAPSVFHLDLGQQASRYQLRKQ |
| Transmembrane helix | 219-241 | VSLRSLFMGGALILALLFGLYHH |
| Outside | 242-259 | QLNNQTQDVLRQLGELLK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9962 | - | - |
Protein sequence: 260 a.a.
>T6CP259527 NZ_JDWB01000021:c66034-65255 [Aeromonas hydrophila] [TssL]
MTTDIIKNEQLSDLLFDHAEQLDMDSDYWFRLRGQSINPMIDAVTPLLGLVQRVRLLSRYDRVPELYQRVVTEIQAIEQE
LMAQGYENGVVLSFRYILCTFIDEAVMGRDWGSQSEWSEHSLLTRFHNETWGGEKVFVLLARLQEDPVRYRDILEFIYLC
LCLGFEGRYKVMSKGQGEFERIVRQLHKQLAPEAGGEAPSVFHLDLGQQASRYQLRKQVSLRSLFMGGALILALLFGLYH
HQLNNQTQDVLRQLGELLK*
MTTDIIKNEQLSDLLFDHAEQLDMDSDYWFRLRGQSINPMIDAVTPLLGLVQRVRLLSRYDRVPELYQRVVTEIQAIEQE
LMAQGYENGVVLSFRYILCTFIDEAVMGRDWGSQSEWSEHSLLTRFHNETWGGEKVFVLLARLQEDPVRYRDILEFIYLC
LCLGFEGRYKVMSKGQGEFERIVRQLHKQLAPEAGGEAPSVFHLDLGQQASRYQLRKQVSLRSLFMGGALILALLFGLYH
HQLNNQTQDVLRQLGELLK*
Nucleotide sequence: 780 bp
>T6CP259527 NZ_JDWB01000021:c66034-65255 [Aeromonas hydrophila] [TssL]
ATGACCACGGACATTATCAAGAACGAGCAACTCAGCGACCTGCTGTTCGATCATGCCGAGCAGCTGGACATGGATTCGGA
CTACTGGTTCCGCCTGCGCGGCCAGAGCATCAACCCGATGATCGATGCGGTGACCCCCTTGCTGGGGCTGGTGCAGCGGG
TACGCCTGCTGTCGCGCTACGACCGGGTGCCCGAGCTCTACCAGCGGGTGGTGACCGAGATCCAGGCCATCGAACAGGAG
CTGATGGCTCAGGGTTACGAGAATGGGGTGGTGCTGTCGTTTCGCTACATCCTCTGCACCTTCATCGACGAGGCCGTGAT
GGGCCGTGACTGGGGCAGTCAGAGCGAATGGTCCGAACACTCCCTGCTGACCCGCTTTCACAACGAGACCTGGGGCGGCG
AGAAGGTGTTCGTGCTGCTGGCCCGCCTGCAGGAAGACCCGGTACGTTATCGCGACATTCTGGAGTTCATCTACCTCTGT
CTGTGCCTCGGTTTCGAGGGGCGCTACAAGGTGATGAGCAAGGGACAAGGGGAGTTCGAGCGCATCGTGCGCCAGCTGCA
CAAGCAGTTGGCCCCCGAGGCCGGCGGCGAGGCGCCCAGCGTATTTCACCTCGATCTGGGTCAGCAGGCCTCCCGCTATC
AGCTGCGCAAGCAGGTTTCCCTGCGCAGCCTGTTCATGGGCGGCGCTCTGATCCTGGCGCTGCTCTTTGGCCTCTATCAC
CACCAACTCAATAACCAGACCCAGGACGTTCTGCGTCAACTGGGCGAATTATTAAAATAA
ATGACCACGGACATTATCAAGAACGAGCAACTCAGCGACCTGCTGTTCGATCATGCCGAGCAGCTGGACATGGATTCGGA
CTACTGGTTCCGCCTGCGCGGCCAGAGCATCAACCCGATGATCGATGCGGTGACCCCCTTGCTGGGGCTGGTGCAGCGGG
TACGCCTGCTGTCGCGCTACGACCGGGTGCCCGAGCTCTACCAGCGGGTGGTGACCGAGATCCAGGCCATCGAACAGGAG
CTGATGGCTCAGGGTTACGAGAATGGGGTGGTGCTGTCGTTTCGCTACATCCTCTGCACCTTCATCGACGAGGCCGTGAT
GGGCCGTGACTGGGGCAGTCAGAGCGAATGGTCCGAACACTCCCTGCTGACCCGCTTTCACAACGAGACCTGGGGCGGCG
AGAAGGTGTTCGTGCTGCTGGCCCGCCTGCAGGAAGACCCGGTACGTTATCGCGACATTCTGGAGTTCATCTACCTCTGT
CTGTGCCTCGGTTTCGAGGGGCGCTACAAGGTGATGAGCAAGGGACAAGGGGAGTTCGAGCGCATCGTGCGCCAGCTGCA
CAAGCAGTTGGCCCCCGAGGCCGGCGGCGAGGCGCCCAGCGTATTTCACCTCGATCTGGGTCAGCAGGCCTCCCGCTATC
AGCTGCGCAAGCAGGTTTCCCTGCGCAGCCTGTTCATGGGCGGCGCTCTGATCCTGGCGCTGCTCTTTGGCCTCTATCAC
CACCAACTCAATAACCAGACCCAGGACGTTCTGCGTCAACTGGGCGAATTATTAAAATAA