Detailed information of component protein


Summary

Component ID T6CP259521
Component protein type TssA
T6SS ID (Type) T6SS17503 experimental (Type i1)
Strain Aeromonas hydrophila strain NF1
Replicon chromosome
Sequence Protein sequence (477 a.a.); Nucleotide sequence (1431 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BE47_RS0109275
Coordinate (Strand) 53988..55418 (-)
NCBI ID WP_029301176.1
RefSeq NZ_JDWB01000021
Uniprot ID UPI0004936CFA
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VasL PF12486.10 5.2e-54 324..470
ImpA_N PF06812.14 5.8e-23 10..115
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-249 MSQNQQGQQALKVGRDPRMLPEYEALRAEINKLSHASRPEVDWQRIHQLASLIFEKHGVDLQTAIYFTLARSRLQGLSGFTEGCEFLANLIVTQWESFWPPVHQERARIEMLDWFIARISDVIRQYQISHEDKRLIYRCERALQLISEKLHNAGLSRIPRVENLVHFIEGYTHLFDETEIVILSDEPGLKEDLQIPPMVFFKSDLESDHGSTVSASTPHLPQGSILVGREKGQVKPTVLKIEQHRRQRP
Transmembrane helix 250-272 AWFWFGCGLLSCALPVMGWLGWQ
Outside 273-476 QHQQEKTVAAQRLVQPAAELPRALSYDDIRQARIVLGEQTLQNMESDLVTRYQNQLTRLEQTSPLYWYRYGEGLRNSLLMLYPDSLAVKALDKQWQTQLGGQQGDVISVPTYLDARAGVDALLDQLLELERQRKTVTISYLKSQLYEVQKNLMQNIPFSLRLGELEARKASQEPITAAELKSLENDLKALNIRLYQLQQGASGS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9958 - -


  Protein sequence: 477 a.a.    

>T6CP259521 NZ_JDWB01000021:c55418-53988 [Aeromonas hydrophila] [TssA]
MSQNQQGQQALKVGRDPRMLPEYEALRAEINKLSHASRPEVDWQRIHQLASLIFEKHGVDLQTAIYFTLARSRLQGLSGF
TEGCEFLANLIVTQWESFWPPVHQERARIEMLDWFIARISDVIRQYQISHEDKRLIYRCERALQLISEKLHNAGLSRIPR
VENLVHFIEGYTHLFDETEIVILSDEPGLKEDLQIPPMVFFKSDLESDHGSTVSASTPHLPQGSILVGREKGQVKPTVLK
IEQHRRQRPAWFWFGCGLLSCALPVMGWLGWQQHQQEKTVAAQRLVQPAAELPRALSYDDIRQARIVLGEQTLQNMESDL
VTRYQNQLTRLEQTSPLYWYRYGEGLRNSLLMLYPDSLAVKALDKQWQTQLGGQQGDVISVPTYLDARAGVDALLDQLLE
LERQRKTVTISYLKSQLYEVQKNLMQNIPFSLRLGELEARKASQEPITAAELKSLENDLKALNIRLYQLQQGASGS*

  Nucleotide sequence: 1431 bp    

>T6CP259521 NZ_JDWB01000021:c55418-53988 [Aeromonas hydrophila] [TssA]
ATGTCACAGAATCAACAAGGCCAGCAGGCCCTGAAAGTGGGGCGCGACCCCAGGATGTTGCCGGAGTACGAGGCGCTGCG
GGCCGAGATCAACAAGCTCAGCCACGCTTCGCGCCCCGAAGTGGACTGGCAGCGGATCCATCAGCTCGCCAGCCTTATCT
TCGAAAAACACGGGGTTGATCTGCAGACCGCCATCTACTTCACCTTGGCCCGTTCACGCCTGCAGGGGCTGAGCGGTTTT
ACCGAAGGATGCGAGTTTCTCGCCAACCTCATCGTCACCCAGTGGGAGAGCTTCTGGCCGCCGGTCCATCAGGAGCGGGC
CCGCATCGAGATGCTCGACTGGTTCATTGCCCGCATCAGCGACGTCATTCGCCAGTACCAGATCAGCCACGAGGACAAGC
GGCTGATCTATCGCTGCGAGCGGGCGCTGCAGCTCATCAGCGAGAAGCTGCACAACGCGGGTCTGAGTCGCATTCCCCGG
GTCGAGAATCTGGTGCACTTCATCGAGGGGTATACCCACCTGTTCGATGAGACCGAGATCGTCATCCTCTCCGACGAGCC
GGGCCTCAAGGAGGATCTGCAGATCCCGCCCATGGTGTTCTTCAAGTCCGACCTGGAGAGTGATCACGGCAGCACGGTCT
CGGCATCGACCCCGCACCTGCCCCAGGGCAGCATTCTGGTGGGGCGCGAGAAGGGGCAGGTCAAGCCCACTGTGCTCAAG
ATTGAGCAGCACCGCCGGCAGCGTCCGGCCTGGTTCTGGTTTGGCTGCGGCCTGCTCAGCTGCGCTCTGCCGGTGATGGG
CTGGCTCGGCTGGCAGCAACATCAGCAGGAGAAAACCGTGGCGGCCCAGCGTCTGGTGCAGCCGGCCGCCGAGTTGCCCC
GAGCCCTCAGCTATGACGACATTCGTCAGGCACGCATCGTGCTGGGTGAGCAGACTTTGCAGAACATGGAGAGCGATCTG
GTGACCCGCTACCAGAACCAGTTGACACGGCTCGAGCAGACATCGCCGCTCTACTGGTATCGCTACGGCGAGGGGCTGCG
CAACTCGCTCTTGATGCTCTATCCCGACTCGCTGGCGGTCAAGGCGCTGGACAAGCAGTGGCAGACGCAGCTTGGCGGCC
AGCAGGGGGATGTCATCAGCGTGCCCACCTACCTGGATGCCCGCGCCGGGGTGGATGCCCTGCTCGATCAGCTGTTGGAG
CTGGAGCGCCAGCGCAAGACGGTGACCATCTCCTACCTCAAATCCCAGCTCTACGAAGTACAGAAGAACCTGATGCAGAA
CATCCCGTTCAGTCTGCGCCTCGGTGAACTGGAGGCGAGAAAAGCCTCCCAGGAGCCCATCACGGCTGCCGAGCTAAAAA
GCCTGGAAAATGATCTGAAAGCGCTCAACATCCGCCTCTATCAGCTGCAGCAGGGCGCTTCCGGTAGCTGA