Experimental investigation has been performed.
Bioinformatics investigation has been performed

T6SS ID	Type	Organism	Replicon (Accession)	Location	Function
T6SS01211	i1	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	1118829..1137804	-
T6SS01212	i4a	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	1913764..1933758	-
T6SS01213	i3	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	5670794..5696508	-

Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0007	Y880_04683 (pnp)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	RsmA regulatory pathway	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	4637120..4639225 (-)	489185761	PA0527.1 (RsmY) (↑); PA3621.1 (RsmZ) (↑)
REG0008	PA0527.1 (RsmY)	T6SS01211 ; T6SS01212 ; T6SS01213	ncRNA	RsmA regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NZ_CP020659)	586867..586990 (+)	-	RsmA (Y880_06257) (↓)
REG0009	PA3621.1 (RsmZ)	T6SS01211 ; T6SS01212 ; T6SS01213	ncRNA	RsmA regulatory pathway	Pseudomonas aeruginosa PAO1	chromosome (NZ_CP020659)	4057543..4057658 (-)	-	RsmA (Y880_06257) (↓)
REG0010	Y880_06257 (rsmA)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	RsmA regulatory pathway	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	6213838..6214644 (-)	510932573	PA4492 mRNA (↑)
REG0011	Y880_05180 (rmsF)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	RsmA regulatory pathway	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	5147809..5148024 (-)	489186811	tssA1 expression (↑)
REG0012	Y880_02189 (gacA/uvrY)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	TCS-RR	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	2199690..2200334 (-)	510933056	hcp expression (↓)
REG0013	Y880_03649 (suhB)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	TCS regulatory pathway	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	3616623..3617438 (+)	489183406	Y880_02189 (gacA/uvrY) (↓)
REG0037	Y880_05519 (dgcP)	T6SS01211 ; T6SS01212 ; T6SS01213	protein	c-di-GMP regulatory pathway	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	5487251..5489266 (-)	489205065	the levels of c-di-GMP (↓)

Experimental investigation has been performed.
Bioinformatics investigation has been performed

Effector ID	Locus tag (Gene)	Classification	Cognate immunity protein	Related T6SS	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID
EFF76647	Y880_RS10855 (tse5)	Tse	IMU38220	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	2306683..2310636 (-)	WP_016253337.1
EFF76642	Y880_RS19070 (tse8)	Tae	IMU38215	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	3986374..3988083 (+)	WP_003101313.1
EFF76643	Y880_RS06360 (tse1)	Tae	IMU38216	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	1318816..1319280 (-)	WP_016253554.1
EFF76644	Y880_RS10940 (tse2)	Tse	IMU38217	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	2330486..2330962 (+)	WP_003119469.1
EFF76645	Y880_RS15550 (tse3)	Tge	IMU38218	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	3243308..3244534 (+)	WP_003092026.1
EFF76646	Y880_RS11390 (tse4)	Tse	IMU38219	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	2409831..2410418 (-)	WP_003099160.1

Experimental investigation has been performed.
Bioinformatics investigation has been performed

Immunity protein ID	Locus tag (Gene)	Related T6SS	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Cognate effector
IMU38215	Y880_RS19075 (tsi8)	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	3988145..3988471 (+)	WP_003093540.1	EFF76646
IMU38216	Y880_RS06365 (tsi1)	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	1319322..1320002 (-)	WP_174243846.1	EFF76646
IMU38217	Y880_RS10945 (tsi2)	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	2330972..2331205 (+)	WP_003114383.1	EFF76646
IMU38218	Y880_RS15555 (tsi3)	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	3244531..3244968 (+)	WP_003113900.1	EFF76646
IMU38219	Y880_RS11395 (tsi4)	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	2410423..2410860 (-)	WP_003099162.1	EFF76646
IMU38220	Y880_RS10850 (tsi5)	T6SS01213	Pseudomonas aeruginosa PAK	chromosome (NZ_CP020659)	2306495..2306686 (-)	WP_003090543.1	EFF76646

Experimental investigation has been performed.
Bioinformatics investigation has been performed

ID	Locus tag (Gene)	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Response to T6SS attack	Known or putative function

Experimental investigation has been performed.
Bioinformatics investigation has been performed

Accessory protein ID	Locus tag (Gene)	Related T6SS	Type	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Related structure protein	Related effector