Detailed information of effector
Summary
| Effector ID | EFF76646  |
| Effector type | Tse |
| Cognate immunity protein | IMU38219 |
| Related T6SS (Type) | T6SS01213 (Type i3) |
| Strain | Pseudomonas aeruginosa PAK |
| Replicon | chromosome |
| Sequence | Protein sequence (196 a.a.); Nucleotide sequence (588 bp) |
| Description | - |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | Y880_RS11390 (tse4) |
| Coordinates (Strand) | 2409831..2410418 (-) |
| NCBI ID | WP_003099160.1 |
| RefSeq | NZ_CP020659 |
| Uniprot ID | A0A1F0J6H9_9PSED, TSE4_PSEAE, A0A072ZTP1_PSEAI |
| KEGG ID | pae:PA2774 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-14 | MSQLPIPLPLNESR |
| Transmembrane helix | 15-37 | WRYVTASGGGMTVAFLAGSGGSI |
| Inside | 38-113 | TLLSPEGENVSFRYGGVGGGIGLGMRLPRFGKVNLNVKGKSVGGAGALEALPSTGTVLVADGLVGRDLSRGDFTGP |
| Transmembrane helix | 114-136 | CMYVELGAGVIAGGSGTAILFGL |
| Outside | 137-139 | DPK |
| Transmembrane helix | 140-159 | LLAAVALASASPLTAALGAS |
| Inside | 160-171 | MSRQLLQSSRGA |
| Transmembrane helix | 172-194 | LLMAGVNVGAQFGGGAAAYLGAL |
| Outside | 195-195 | F |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.5930 | - | - |
Protein sequence: 196 a.a. .
>EFF76646 NZ_CP020659:c2410418-2409831 Type VI Secretion System Effector (Tse) [Pseudomonas aeruginosa PAK]
MSQLPIPLPLNESRWRYVTASGGGMTVAFLAGSGGSITLLSPEGENVSFRYGGVGGGIGLGMRLPRFGKVNLNVKGKSVG
GAGALEALPSTGTVLVADGLVGRDLSRGDFTGPCMYVELGAGVIAGGSGTAILFGLDPKLLAAVALASASPLTAALGASM
SRQLLQSSRGALLMAGVNVGAQFGGGAAAYLGALF*
MSQLPIPLPLNESRWRYVTASGGGMTVAFLAGSGGSITLLSPEGENVSFRYGGVGGGIGLGMRLPRFGKVNLNVKGKSVG
GAGALEALPSTGTVLVADGLVGRDLSRGDFTGPCMYVELGAGVIAGGSGTAILFGLDPKLLAAVALASASPLTAALGASM
SRQLLQSSRGALLMAGVNVGAQFGGGAAAYLGALF*
Nucleotide sequence: 588 bp .
>EFF76646 NZ_CP020659:c2410418-2409831 Type VI Secretion System Effector (Tse) [Pseudomonas aeruginosa PAK]
ATGTCGCAACTTCCCATTCCCTTGCCCCTCAATGAGAGTCGCTGGCGCTACGTGACGGCCAGTGGCGGAGGCATGACCGT
GGCCTTCCTGGCGGGTAGCGGCGGCTCCATCACGTTGCTCTCGCCGGAGGGTGAGAACGTGTCCTTCCGCTATGGCGGGG
TGGGCGGCGGGATCGGACTGGGCATGCGCCTGCCGCGCTTCGGCAAGGTCAACCTCAATGTCAAGGGCAAGAGCGTTGGC
GGGGCCGGTGCGCTGGAGGCGTTGCCGAGCACCGGAACCGTCTTGGTCGCGGACGGCCTGGTGGGGCGCGACCTGTCCCG
CGGCGACTTCACCGGGCCCTGCATGTACGTCGAACTCGGCGCGGGAGTCATCGCCGGCGGTTCGGGCACGGCGATCCTCT
TCGGCCTCGATCCCAAGCTGCTGGCGGCGGTGGCGCTGGCGAGCGCCTCGCCATTGACCGCCGCGCTGGGCGCCAGCATG
TCGCGGCAATTACTGCAATCGTCCCGTGGCGCCCTGTTGATGGCCGGGGTCAACGTCGGTGCACAGTTCGGCGGCGGCGC
GGCGGCTTACCTGGGGGCGTTGTTCTGA
ATGTCGCAACTTCCCATTCCCTTGCCCCTCAATGAGAGTCGCTGGCGCTACGTGACGGCCAGTGGCGGAGGCATGACCGT
GGCCTTCCTGGCGGGTAGCGGCGGCTCCATCACGTTGCTCTCGCCGGAGGGTGAGAACGTGTCCTTCCGCTATGGCGGGG
TGGGCGGCGGGATCGGACTGGGCATGCGCCTGCCGCGCTTCGGCAAGGTCAACCTCAATGTCAAGGGCAAGAGCGTTGGC
GGGGCCGGTGCGCTGGAGGCGTTGCCGAGCACCGGAACCGTCTTGGTCGCGGACGGCCTGGTGGGGCGCGACCTGTCCCG
CGGCGACTTCACCGGGCCCTGCATGTACGTCGAACTCGGCGCGGGAGTCATCGCCGGCGGTTCGGGCACGGCGATCCTCT
TCGGCCTCGATCCCAAGCTGCTGGCGGCGGTGGCGCTGGCGAGCGCCTCGCCATTGACCGCCGCGCTGGGCGCCAGCATG
TCGCGGCAATTACTGCAATCGTCCCGTGGCGCCCTGTTGATGGCCGGGGTCAACGTCGGTGCACAGTTCGGCGGCGGCGC
GGCGGCTTACCTGGGGGCGTTGTTCTGA