Detailed information of effector


Summary

Effector ID EFF76647 experimental
Effector type Tse
Cognate immunity protein IMU38220 experimental
Related T6SS (Type) T6SS01213 experimental (Type i3)
Strain Pseudomonas aeruginosa PAK
Replicon chromosome
Sequence Protein sequence (1318 a.a.); Nucleotide sequence (3954 bp)
Description -
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) Y880_RS10855 (tse5)
Coordinates (Strand) 2306683..2310636 (-)
NCBI ID WP_016253337.1
RefSeq NZ_CP020659
Uniprot ID UPI000334C1C3
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
RHS PF03527.16 4.6e-15 1065..1102
DUF6531 PF20148.1 1e-13 46..120
RHS_repeat PF05593.16 5.1e-08 489..524
RHS_repeat PF05593.16 1.4e-06 531..566
RHS_repeat PF05593.16 3.5e-06 595..630
RHS_repeat PF05593.16 5.3e-06 636..670
RHS_repeat PF05593.16 8.1e-05 927..965
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1317 MSGLPVSHVGEKVSGGVISTGSPTVHVGSSAVGLADRVSACVPLVGKPVNPMLGSKLLPEEVDFALAAPDTFTFARGYLSSNPRIGRLGRGWWLPGESMHLELSEDACVLVDAQGRRIGFPALAPGAQHYSGSEELWLRRGGSSGGEAQAWRGRWAAVPAELQTQEGSVLVLSGHSYLHFQRCPDGIWRLQASFGRAGYRTEFRWSGRGLLTGVRDSAGRSYALVYQQACEPSEGDDGLRLFGVILASHDGPPPDYIDPQSPGLDWLVRYQFSDSGDLIAVRDRLGQVVRVFAWREHMLVAHGEPGGLEVRYEWDVHAPHGRVVKQIEAGGLTRTFRYLRDATEVSDSLGRVERYEFAGEGGQRRWTALVRADGSRSEFDYDLFGRLVTMRDPLGRETRRRRDGQGRMLEEESPGKARYRKRVDEETGLLVELEDAMQRRWTFERDERGNATTVRGPAGSTRYAYEDPRLPDRPTRIVDPRGGERRLEWNRFGLLAALTDCSGQVWRYDYDNEGRLVASSDPLGQLTRRRYDPLGQLIGLELADGSALSYEYDALGRQTRIADAEGHATLFSWGHGDLLVRVSDAGGGELSYLHDEAGRLVALTNENGVQAQFRYDLLDRLVEETGFDGRRQRYRYNAADELIAREDADGRETTYAYDRDGRLASIRVPATEHAPALVERYRWLADGRLASAGGADCEVRYTYDEVGNLRLESQVHADGWVYSVEHSHDALGVRQTSRYGDAPPVAWLTYGPGHLHGALVGAVELAFERDALHREVRRDARRDGQDDALFTQERQHAPLGRLQRSRLRLAGGFDWQRGYRYDGLGQLVGIDDNQYLSVRYEYDLGGRLLASRRAGAAASTYRYDAAGNRLEGVGEYAREDARQAFAENELYRSGFSRSEARANQAGEGPARWAGNRVERIAGNRYRFDALGNLVERIGADGERLRLAYDGAQRLVHLTRDYADGTRLEARYRYDALSRRIAKVVLRDGVEQQVRFGWDGDRQCAEAFARELRTTVHEPGGFVPLLRLEQACEPDPPELLQLRQAFAAEGQPLPAQCVPALGEARIAFFHTDHLGTPLQLSDERGQLRWQGVPDDWRAVAPERQPGAQPIRFQGQYHDEESGLYYNRYRYYLPEAGRYASQDPLGLGGGPNPYAYALNAPTLAYDPTGLIIPLVVIGAFAARAAIGAALGAGIELGMQTGKQVLGQMKDNWDSDRDLTDIKWKCIDINWKHVGASAAIGTVAPGMLSTGKTVVQSAKAIRTLSGQAANTANRAAKLAARKAAHADTIKKAVATQAAWQTGKQIVKCPLKDEEEECPPQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9318 - -


  Protein sequence: 1318 a.a.    .

>EFF76647 NZ_CP020659:c2310636-2306683 Type VI Secretion System Effector (Tse) [Pseudomonas aeruginosa PAK]
MSGLPVSHVGEKVSGGVISTGSPTVHVGSSAVGLADRVSACVPLVGKPVNPMLGSKLLPEEVDFALAAPDTFTFARGYLS
SNPRIGRLGRGWWLPGESMHLELSEDACVLVDAQGRRIGFPALAPGAQHYSGSEELWLRRGGSSGGEAQAWRGRWAAVPA
ELQTQEGSVLVLSGHSYLHFQRCPDGIWRLQASFGRAGYRTEFRWSGRGLLTGVRDSAGRSYALVYQQACEPSEGDDGLR
LFGVILASHDGPPPDYIDPQSPGLDWLVRYQFSDSGDLIAVRDRLGQVVRVFAWREHMLVAHGEPGGLEVRYEWDVHAPH
GRVVKQIEAGGLTRTFRYLRDATEVSDSLGRVERYEFAGEGGQRRWTALVRADGSRSEFDYDLFGRLVTMRDPLGRETRR
RRDGQGRMLEEESPGKARYRKRVDEETGLLVELEDAMQRRWTFERDERGNATTVRGPAGSTRYAYEDPRLPDRPTRIVDP
RGGERRLEWNRFGLLAALTDCSGQVWRYDYDNEGRLVASSDPLGQLTRRRYDPLGQLIGLELADGSALSYEYDALGRQTR
IADAEGHATLFSWGHGDLLVRVSDAGGGELSYLHDEAGRLVALTNENGVQAQFRYDLLDRLVEETGFDGRRQRYRYNAAD
ELIAREDADGRETTYAYDRDGRLASIRVPATEHAPALVERYRWLADGRLASAGGADCEVRYTYDEVGNLRLESQVHADGW
VYSVEHSHDALGVRQTSRYGDAPPVAWLTYGPGHLHGALVGAVELAFERDALHREVRRDARRDGQDDALFTQERQHAPLG
RLQRSRLRLAGGFDWQRGYRYDGLGQLVGIDDNQYLSVRYEYDLGGRLLASRRAGAAASTYRYDAAGNRLEGVGEYARED
ARQAFAENELYRSGFSRSEARANQAGEGPARWAGNRVERIAGNRYRFDALGNLVERIGADGERLRLAYDGAQRLVHLTRD
YADGTRLEARYRYDALSRRIAKVVLRDGVEQQVRFGWDGDRQCAEAFARELRTTVHEPGGFVPLLRLEQACEPDPPELLQ
LRQAFAAEGQPLPAQCVPALGEARIAFFHTDHLGTPLQLSDERGQLRWQGVPDDWRAVAPERQPGAQPIRFQGQYHDEES
GLYYNRYRYYLPEAGRYASQDPLGLGGGPNPYAYALNAPTLAYDPTGLIIPLVVIGAFAARAAIGAALGAGIELGMQTGK
QVLGQMKDNWDSDRDLTDIKWKCIDINWKHVGASAAIGTVAPGMLSTGKTVVQSAKAIRTLSGQAANTANRAAKLAARKA
AHADTIKKAVATQAAWQTGKQIVKCPLKDEEEECPPQ*

  Nucleotide sequence: 3954 bp    .

>EFF76647 NZ_CP020659:c2310636-2306683 Type VI Secretion System Effector (Tse) [Pseudomonas aeruginosa PAK]
ATGAGCGGCCTACCGGTTTCCCATGTCGGCGAGAAAGTCTCGGGAGGGGTTATCTCCACCGGCTCGCCCACCGTGCACGT
CGGCAGCAGCGCGGTCGGCCTGGCCGACCGGGTGTCCGCCTGCGTGCCGTTGGTGGGCAAGCCGGTCAACCCGATGCTCG
GCAGCAAGCTGCTGCCGGAAGAGGTCGATTTCGCCCTGGCGGCGCCGGACACCTTCACCTTCGCCCGTGGCTACCTGTCG
AGCAACCCCCGTATCGGCCGGCTCGGCCGGGGCTGGTGGCTGCCGGGCGAGAGCATGCACCTGGAACTGAGCGAAGACGC
CTGCGTGCTGGTCGACGCCCAGGGCCGCCGGATCGGCTTTCCTGCGCTGGCTCCCGGTGCCCAGCATTATTCCGGCAGCG
AGGAGCTGTGGCTGCGCCGCGGCGGTTCGAGCGGCGGCGAAGCGCAGGCCTGGCGCGGGCGCTGGGCGGCGGTGCCGGCC
GAACTGCAGACGCAGGAGGGCTCCGTGCTGGTGCTGTCCGGCCACAGCTACCTGCATTTCCAGCGTTGCCCCGACGGTAT
CTGGCGCCTGCAGGCCAGCTTCGGCCGCGCCGGCTACCGCACCGAGTTCCGCTGGAGCGGACGTGGCCTGCTCACCGGCG
TGCGCGACAGCGCCGGGCGCAGCTATGCGCTGGTCTACCAGCAAGCCTGCGAGCCGAGCGAGGGCGACGACGGCCTGCGT
CTGTTCGGCGTGATCCTGGCCAGCCACGACGGACCGCCTCCGGACTACATCGATCCGCAGAGCCCGGGGCTCGACTGGCT
GGTGCGCTACCAGTTCAGCGACAGCGGCGATCTGATCGCCGTGCGCGACCGTCTTGGCCAGGTGGTCCGGGTATTCGCCT
GGCGCGAGCACATGCTGGTCGCCCACGGCGAACCGGGCGGGCTGGAGGTTCGCTACGAGTGGGATGTCCATGCTCCGCAC
GGGCGGGTCGTGAAGCAGATCGAGGCGGGCGGCCTGACCCGTACCTTCCGCTACCTGCGCGATGCCACCGAGGTCAGCGA
CAGCCTCGGCCGCGTCGAACGCTACGAGTTCGCCGGCGAGGGCGGGCAGCGGCGCTGGACCGCGCTGGTCAGGGCCGACG
GCAGCCGTAGCGAGTTCGACTACGACCTGTTCGGCCGCCTGGTCACCATGCGCGATCCGCTGGGACGGGAGACACGGCGC
CGGCGCGATGGCCAGGGGCGGATGCTCGAGGAGGAGTCGCCGGGCAAGGCGCGCTATCGCAAGCGCGTCGACGAAGAGAC
CGGGCTGCTGGTCGAGCTGGAGGACGCGATGCAGCGCCGCTGGACCTTCGAGCGCGACGAACGGGGCAATGCGACGACGG
TGCGCGGCCCGGCCGGCAGCACCCGCTACGCCTACGAAGACCCGCGCCTGCCCGACCGGCCGACGCGAATCGTCGACCCG
CGCGGCGGCGAGCGGCGCCTGGAGTGGAACCGCTTCGGCCTGTTGGCCGCGTTGACCGATTGTTCCGGCCAGGTCTGGCG
TTACGACTACGACAACGAAGGACGTTTGGTCGCCAGCAGCGACCCGCTGGGACAACTGACCCGGCGCCGCTACGACCCGC
TGGGGCAACTGATCGGCCTGGAATTGGCCGACGGCAGCGCCCTGAGCTATGAATACGACGCCCTTGGGCGGCAGACCCGT
ATCGCCGATGCCGAGGGGCACGCCACGCTGTTTTCCTGGGGGCACGGCGATCTGCTGGTGCGGGTTTCCGACGCCGGCGG
CGGCGAATTGTCCTACCTGCACGACGAAGCCGGCAGGCTGGTCGCCCTGACCAACGAGAACGGCGTCCAGGCGCAGTTCC
GCTACGACCTGCTGGACCGTCTGGTGGAAGAGACCGGCTTCGACGGTCGTCGCCAACGCTATCGCTACAACGCCGCTGAC
GAACTGATCGCCCGCGAGGATGCCGACGGTCGCGAGACCACCTATGCCTACGACCGCGACGGCCGCCTGGCGAGCATCCG
GGTTCCCGCCACCGAGCACGCACCGGCCCTGGTCGAGCGTTACCGCTGGTTGGCCGACGGCCGCCTGGCCAGCGCCGGCG
GGGCGGATTGCGAGGTGCGCTACACGTACGACGAGGTCGGTAACCTGCGCCTGGAAAGCCAGGTACATGCCGACGGTTGG
GTCTACAGCGTCGAGCACAGCCACGACGCGCTAGGCGTGCGCCAGACCAGCCGCTACGGCGACGCGCCGCCGGTCGCCTG
GCTGACCTATGGCCCGGGTCATCTGCACGGCGCGCTGGTCGGTGCGGTCGAGCTGGCCTTCGAGCGCGATGCCCTGCACC
GTGAGGTGCGTCGCGACGCCCGCCGCGACGGGCAGGATGACGCGTTGTTCACCCAGGAGCGCCAGCACGCGCCGCTGGGA
CGCCTGCAGCGCAGCCGCCTGCGTCTGGCCGGCGGTTTCGACTGGCAGCGCGGCTATCGCTACGACGGACTTGGACAACT
GGTCGGCATCGACGACAACCAGTATCTGTCGGTGCGCTACGAATACGACCTCGGCGGTCGCCTGCTGGCCAGCCGGCGCG
CTGGCGCGGCGGCCAGCACCTACCGCTACGACGCCGCCGGCAACCGCCTGGAGGGCGTCGGCGAGTACGCGCGCGAGGAC
GCTCGCCAGGCATTCGCCGAGAACGAACTGTACCGCTCCGGTTTCAGTCGCTCCGAGGCGCGCGCGAACCAGGCCGGCGA
AGGCCCGGCGCGCTGGGCCGGCAACCGCGTGGAGAGGATCGCCGGCAACCGCTATCGCTTCGATGCGCTGGGCAACCTGG
TCGAGCGTATCGGCGCCGACGGCGAGCGCCTGCGCCTGGCCTACGACGGCGCGCAGCGCCTGGTCCATCTGACGCGCGAT
TACGCCGACGGCACGCGGCTGGAGGCGCGCTACCGCTACGACGCGCTGTCGCGACGGATCGCCAAGGTGGTGCTGCGCGA
CGGTGTCGAGCAGCAGGTGCGCTTCGGCTGGGACGGCGATCGCCAATGCGCCGAAGCCTTTGCGCGGGAACTGCGCACCA
CTGTTCACGAACCGGGCGGTTTCGTCCCGTTGCTGCGCCTGGAGCAGGCCTGCGAGCCCGATCCCCCGGAGCTGCTGCAA
CTGCGCCAGGCGTTCGCCGCCGAAGGCCAGCCGCTGCCGGCGCAATGCGTGCCGGCGCTGGGCGAGGCGCGGATCGCTTT
CTTCCACACCGATCACCTCGGTACCCCCCTGCAGCTGAGCGACGAACGTGGCCAACTGCGTTGGCAGGGCGTTCCGGACG
ACTGGCGGGCGGTAGCGCCGGAACGACAGCCGGGTGCGCAGCCGATCCGCTTCCAGGGGCAGTACCACGATGAGGAAAGC
GGCCTCTACTACAACCGCTACCGTTACTACCTGCCGGAAGCCGGGCGCTATGCCAGCCAGGACCCGCTCGGCCTGGGCGG
TGGCCCCAACCCCTATGCCTACGCGCTGAACGCGCCCACGCTGGCCTACGATCCCACCGGCCTGATCATCCCGCTGGTGG
TGATCGGCGCCTTCGCCGCCCGCGCCGCGATCGGAGCGGCGCTGGGCGCCGGCATCGAGCTGGGGATGCAAACCGGCAAG
CAGGTGCTGGGGCAGATGAAGGACAACTGGGACAGCGATCGCGACCTCACCGATATCAAGTGGAAGTGCATCGACATCAA
CTGGAAACACGTCGGCGCCTCGGCGGCCATCGGCACCGTGGCGCCGGGCATGCTCAGCACCGGCAAGACCGTGGTGCAGT
CCGCCAAGGCGATCAGGACCCTTTCCGGACAGGCGGCCAACACCGCCAACCGCGCGGCCAAGCTGGCCGCGCGCAAGGCC
GCCCATGCCGATACCATCAAGAAGGCCGTGGCCACCCAGGCCGCCTGGCAGACGGGCAAGCAGATCGTCAAATGCCCGCT
GAAGGACGAAGAAGAGGAGTGCCCGCCGCAATGA