Detailed information of regulator
Summary
| Regulator ID | REG0010  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | RsmA regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS01211 (Type i1); T6SS01212 (Type i4a); T6SS01213 (Type i3) |
| Strain | Pseudomonas aeruginosa PAK |
| Replicon | chromosome |
| Sequence | Protein sequence (269 a.a.); Nucleotide sequence (807 bp) |
| Description | Deletion of gacA or rsmY/rsmZ reduced the level of Hcp1 in the suhB mutant background. In addition, overexpression of rsmA suppressed the expression of Hcp1 in the suhB mutant. |
| Reference | [1] Brencic A, Lory S. Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Mol Microbiol. 2009 May;72(3):612-32. doi: 10.1111/j.1365-2958.2009.06670.x. PMID: 19426209 [2] Li K, Xu C, Jin Y, et al. SuhB is a regulator of multiple virulence genes and essential for pathogenesis of Pseudomonas aeruginosa. mBio. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13. PMID: 24169572 |
External database links
| Locus tag (Gene) | Y880_06257 (rsmA) |
| Coordinates (Strand) | 6213838..6214644 (-) |
| NCBI ID | 510932573 |
| RefSeq | NZ_CP020659 |
| Uniprot ID | UPI000339D6FC |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| PA4492 mRNA | ↑ | cgcacgaccagggcttgatcatgcgtatcttcggttcgagtgccggctgcctgtcggtgctgttcggactggccttcgggctgcctgcactagccgagccttcggtggacctggatggtccgacggcaggctggcgctattccggcctgctcgagcagcccgatgccccgcg Red: sequences identified in RNA–RsmA‐H6 co‐purification assay |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| RrnaAD | PF00398.22 | 3.6e-64 | 7..259 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-268 | MSELYQHRARKRFGQNFLHDAGVIHRILRAIHAREGQRLLEIGPGQGALTEGLLGSGARLDVIELDQDLIPLLKLKFGLESRFSLHQGDALKFDFASLVESGEKLRVVGNLPYNISTPLIFHLLEYAPVIEDMHFMLQKEVVERLAATPGGGDWGRLSIMVQYHCRVEHLFNVGPGAFNPPPKVDSAIVRLTPFAEPPHPARDPKLLERVVREAFNQRRKTLRNTLKPLLSVEDIEAAEVDPTLRPEQLDLTAFVRLANRLAELPGNR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9929 | - | - |
Protein sequence of regulator: 269 a.a. .
>REG0010 NZ_CP020659:c6214644-6213838 [Pseudomonas aeruginosa PAK]
MSELYQHRARKRFGQNFLHDAGVIHRILRAIHAREGQRLLEIGPGQGALTEGLLGSGARLDVIELDQDLIPLLKLKFGLE
SRFSLHQGDALKFDFASLVESGEKLRVVGNLPYNISTPLIFHLLEYAPVIEDMHFMLQKEVVERLAATPGGGDWGRLSIM
VQYHCRVEHLFNVGPGAFNPPPKVDSAIVRLTPFAEPPHPARDPKLLERVVREAFNQRRKTLRNTLKPLLSVEDIEAAEV
DPTLRPEQLDLTAFVRLANRLAELPGNR*
MSELYQHRARKRFGQNFLHDAGVIHRILRAIHAREGQRLLEIGPGQGALTEGLLGSGARLDVIELDQDLIPLLKLKFGLE
SRFSLHQGDALKFDFASLVESGEKLRVVGNLPYNISTPLIFHLLEYAPVIEDMHFMLQKEVVERLAATPGGGDWGRLSIM
VQYHCRVEHLFNVGPGAFNPPPKVDSAIVRLTPFAEPPHPARDPKLLERVVREAFNQRRKTLRNTLKPLLSVEDIEAAEV
DPTLRPEQLDLTAFVRLANRLAELPGNR*
Nucleotide sequence of regulator: 807 bp .
>REG0010 NZ_CP020659:c6214644-6213838 [Pseudomonas aeruginosa PAK]
ATGTCCGAGCTTTACCAACACCGCGCGCGCAAGCGCTTCGGCCAGAACTTCCTGCACGATGCCGGGGTGATTCACCGCAT
CCTCCGTGCCATCCATGCCCGCGAGGGTCAGCGCCTGCTGGAGATCGGCCCGGGCCAGGGCGCCCTGACCGAGGGACTGC
TGGGCAGCGGCGCACGCCTGGACGTGATCGAGCTCGACCAGGACCTGATCCCCCTGTTGAAGCTGAAATTCGGCCTCGAG
TCGCGCTTCAGCCTGCACCAGGGCGACGCCCTCAAGTTCGATTTCGCCTCGCTGGTGGAGAGCGGCGAGAAGCTGCGAGT
GGTCGGCAACCTGCCCTACAACATCTCCACGCCACTGATCTTCCATCTGCTGGAGTACGCGCCGGTGATCGAGGACATGC
ACTTCATGCTGCAGAAGGAAGTGGTCGAGCGCCTGGCGGCCACGCCGGGCGGCGGAGACTGGGGCCGGCTCTCGATCATG
GTCCAGTATCACTGTCGGGTGGAGCACCTGTTCAACGTCGGCCCCGGCGCGTTCAACCCGCCGCCCAAGGTCGACTCGGC
CATCGTCCGTCTCACCCCCTTCGCCGAGCCGCCCCATCCGGCACGCGATCCGAAACTGCTCGAGCGGGTGGTCCGCGAGG
CCTTCAACCAGCGCCGCAAGACCCTGCGCAATACCCTCAAGCCTCTCCTGAGCGTCGAGGACATCGAGGCCGCAGAGGTC
GATCCGACCCTCCGTCCCGAGCAACTCGACCTGACCGCATTCGTCCGCCTGGCCAACCGGCTGGCCGAGCTGCCAGGCAA
TCGTTAG
ATGTCCGAGCTTTACCAACACCGCGCGCGCAAGCGCTTCGGCCAGAACTTCCTGCACGATGCCGGGGTGATTCACCGCAT
CCTCCGTGCCATCCATGCCCGCGAGGGTCAGCGCCTGCTGGAGATCGGCCCGGGCCAGGGCGCCCTGACCGAGGGACTGC
TGGGCAGCGGCGCACGCCTGGACGTGATCGAGCTCGACCAGGACCTGATCCCCCTGTTGAAGCTGAAATTCGGCCTCGAG
TCGCGCTTCAGCCTGCACCAGGGCGACGCCCTCAAGTTCGATTTCGCCTCGCTGGTGGAGAGCGGCGAGAAGCTGCGAGT
GGTCGGCAACCTGCCCTACAACATCTCCACGCCACTGATCTTCCATCTGCTGGAGTACGCGCCGGTGATCGAGGACATGC
ACTTCATGCTGCAGAAGGAAGTGGTCGAGCGCCTGGCGGCCACGCCGGGCGGCGGAGACTGGGGCCGGCTCTCGATCATG
GTCCAGTATCACTGTCGGGTGGAGCACCTGTTCAACGTCGGCCCCGGCGCGTTCAACCCGCCGCCCAAGGTCGACTCGGC
CATCGTCCGTCTCACCCCCTTCGCCGAGCCGCCCCATCCGGCACGCGATCCGAAACTGCTCGAGCGGGTGGTCCGCGAGG
CCTTCAACCAGCGCCGCAAGACCCTGCGCAATACCCTCAAGCCTCTCCTGAGCGTCGAGGACATCGAGGCCGCAGAGGTC
GATCCGACCCTCCGTCCCGAGCAACTCGACCTGACCGCATTCGTCCGCCTGGCCAACCGGCTGGCCGAGCTGCCAGGCAA
TCGTTAG