Detailed information of regulator
Summary
| Regulator ID | REG0011  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | RsmA regulatory pathway |
| KEGG pathway (determined by BlastKOALA) | Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Pseudomonas aeruginosa (map02025); Biofilm formation - Escherichia coli (map02026) |
| Related T6SS | T6SS01211 (Type i1); T6SS01212 (Type i4a); T6SS01213 (Type i3) |
| Strain | Pseudomonas aeruginosa PAK |
| Replicon | chromosome |
| Sequence | Protein sequence (72 a.a.); Nucleotide sequence (216 bp) |
| Description | Compared with wild-type PA103, PtssA1-lacZ transcriptional and translational reporter activity remained unaffected in the rsmF mutant, but was slightly derepressed in the rsmA mutant and significantly derepressed in an rsmAF mutant. Complementation of the rsmAF mutant with either plasmid-encoded RsmA or RsmF restored repression of PtssA1-lacZ and PtssA1’-‘lacZ reporter activities. |
| Reference |
External database links
| Locus tag (Gene) | Y880_05180 (rmsF) |
| Coordinates (Strand) | 5147809..5148024 (-) |
| NCBI ID | 489186811 |
| RefSeq | NZ_CP020659 |
| Uniprot ID | S5ZIQ4_PSEA1, V6ANM7_PSEAI, A0A1C7BD30_PSEAI, A0A1F0I7X6_9PSED, A0A0H2ZIZ8_PSEAB |
| KEGG ID | pau:PA14_68470 |
| KEGG pathway (determined by BlastKOALA) | map02020; map05111; map02025; map02026 |
| PDB ID | 4K59, 4KJI, 4KRW |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| tssA1 expression | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| CsrA | PF02599.18 | 2.1e-10 | 4..67 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-71 | MGFLILSRREGEGITLSLKADYPAEELIRQLREGGIRILVTDIIGNQARVGIEAPRGVLIVRDELKTAPKG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9900 | - | - |
Protein sequence of regulator: 72 a.a. .
>REG0011 NZ_CP020659:c5148024-5147809 [Pseudomonas aeruginosa PAK]
MGFLILSRREGEGITLSLKADYPAEELIRQLREGGIRILVTDIIGNQARVGIEAPRGVLIVRDELKTAPKG*
MGFLILSRREGEGITLSLKADYPAEELIRQLREGGIRILVTDIIGNQARVGIEAPRGVLIVRDELKTAPKG*
Nucleotide sequence of regulator: 216 bp .
>REG0011 NZ_CP020659:c5148024-5147809 [Pseudomonas aeruginosa PAK]
ATGGGTTTCCTGATACTCTCCCGCCGAGAAGGCGAAGGCATCACCCTGTCCCTCAAGGCCGACTACCCGGCGGAGGAACT
GATTCGGCAATTGCGCGAAGGCGGCATCCGGATCCTGGTCACCGATATCATCGGCAACCAGGCCCGGGTCGGGATCGAGG
CGCCGCGTGGTGTCCTGATCGTTCGCGACGAGTTGAAAACGGCACCGAAAGGCTGA
ATGGGTTTCCTGATACTCTCCCGCCGAGAAGGCGAAGGCATCACCCTGTCCCTCAAGGCCGACTACCCGGCGGAGGAACT
GATTCGGCAATTGCGCGAAGGCGGCATCCGGATCCTGGTCACCGATATCATCGGCAACCAGGCCCGGGTCGGGATCGAGG
CGCCGCGTGGTGTCCTGATCGTTCGCGACGAGTTGAAAACGGCACCGAAAGGCTGA