Detailed information of immunity protein
Summary
| Immunity protein ID | IMU38215  |
| Cognate effector | EFF76642 |
| Related T6SS (Type) | T6SS01213 (Type i3) |
| Strain | Pseudomonas aeruginosa PAK |
| Replicon | chromosome |
| Sequence | Protein sequence (109 a.a.); Nucleotide sequence (327 bp) |
| Description | The PAKΔretS strain lacking either tsei8 or tse8 could be complemented in a competition assay by expression of tsei8 from pBBR-MCS5 or tse8 from pBBR-MCS4. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | Y880_RS19075 (tsi8) |
| Coordinates (Strand) | 3988145..3988471 (+) |
| NCBI ID | WP_003093540.1 |
| RefSeq | NZ_CP020659 |
| Uniprot ID | V6A9X8_PSEAI, A0A1F0IAB1_9PSED, A0A0H2ZG99_PSEAB, A0A081HJU1_PSEAI, A0A3S4Q300_PSEFL |
| KEGG ID | pau:PA14_10020 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| GGACT | PF06094.14 | 1.9e-15 | 4..106 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-108 | MQRLFVYGSLGPGRPNEHLMQKIGGQWQAASLRGRLLEKGWGAGMGFPGLAIDADGEEIHGHVFVSDRLAEHWPALDEFEGTEYRRVATRVTLADGAQVDAYVYALRD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9652 | - | - |
Protein sequence: 109 a.a. .
>IMU38215 NZ_CP020659:3988145-3988471 [Pseudomonas aeruginosa PAK]
MQRLFVYGSLGPGRPNEHLMQKIGGQWQAASLRGRLLEKGWGAGMGFPGLAIDADGEEIHGHVFVSDRLAEHWPALDEFE
GTEYRRVATRVTLADGAQVDAYVYALRD*
MQRLFVYGSLGPGRPNEHLMQKIGGQWQAASLRGRLLEKGWGAGMGFPGLAIDADGEEIHGHVFVSDRLAEHWPALDEFE
GTEYRRVATRVTLADGAQVDAYVYALRD*
Nucleotide sequence: 327 bp .
>IMU38215 NZ_CP020659:3988145-3988471 [Pseudomonas aeruginosa PAK]
ATGCAGCGACTCTTCGTCTACGGCAGCCTCGGCCCCGGCCGTCCCAACGAACACCTGATGCAGAAGATCGGCGGGCAATG
GCAGGCGGCCAGCCTCCGCGGTCGCCTGCTGGAAAAGGGCTGGGGTGCCGGGATGGGCTTTCCGGGCCTGGCGATCGACG
CCGACGGCGAGGAAATCCACGGCCATGTGTTCGTCTCCGACCGCCTCGCCGAGCACTGGCCCGCGCTGGATGAATTCGAA
GGCACCGAATACCGGCGCGTGGCGACCCGGGTAACGCTGGCCGACGGCGCCCAGGTCGATGCCTACGTCTACGCCCTGCG
CGACTGA
ATGCAGCGACTCTTCGTCTACGGCAGCCTCGGCCCCGGCCGTCCCAACGAACACCTGATGCAGAAGATCGGCGGGCAATG
GCAGGCGGCCAGCCTCCGCGGTCGCCTGCTGGAAAAGGGCTGGGGTGCCGGGATGGGCTTTCCGGGCCTGGCGATCGACG
CCGACGGCGAGGAAATCCACGGCCATGTGTTCGTCTCCGACCGCCTCGCCGAGCACTGGCCCGCGCTGGATGAATTCGAA
GGCACCGAATACCGGCGCGTGGCGACCCGGGTAACGCTGGCCGACGGCGCCCAGGTCGATGCCTACGTCTACGCCCTGCG
CGACTGA