NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	PWP91_RS10625	Genome accession	NZ_CP118738
Coordinates	2130941..2131588 (-)	Length	215 a.a.
NCBI ID	WP_058223210.1	Uniprot ID	A0AAP3Z0X8
Organism	Lactococcus lactis strain P-1
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2125941..2136588

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWP91_RS10600 (PWP91_10600)	atpF	2126010..2126516 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
PWP91_RS10605 (PWP91_10605)	atpB	2126531..2127244 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
PWP91_RS10610 (PWP91_10610)	-	2127289..2127504 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
PWP91_RS10615 (PWP91_10615)	-	2127689..2128465 (-)	777	WP_274998509.1	alpha/beta hydrolase	-
PWP91_RS10620 (PWP91_10620)	comEC	2128750..2130960 (-)	2211	WP_274998511.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PWP91_RS10625 (PWP91_10625)	comEA	2130941..2131588 (-)	648	WP_058223210.1	ComEA family DNA-binding protein	Machinery gene
PWP91_RS10630 (PWP91_10630)	-	2131648..2133009 (-)	1362	WP_058211667.1	ABC transporter permease	-
PWP91_RS10635 (PWP91_10635)	-	2133006..2133938 (-)	933	WP_058203963.1	ABC transporter ATP-binding protein	-
PWP91_RS10640 (PWP91_10640)	-	2134043..2134441 (-)	399	WP_004255236.1	hypothetical protein	-
PWP91_RS10645 (PWP91_10645)	-	2134545..2135108 (-)	564	WP_274998513.1	GNAT family N-acetyltransferase	-
PWP91_RS10650 (PWP91_10650)	-	2135281..2136459 (-)	1179	WP_274998514.1	SLC13 family permease	-

Sequence

Protein

Download Length: 215 a.a. Molecular weight: 23610.74 Da Isoelectric Point: 4.8659

                                >NTDB_id=794817 PWP91_RS10625 WP_058223210.1 2130941..2131588(-) (comEA) [Lactococcus lactis strain P-1]

MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP

NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD

VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

Download Length: 648 bp

                                >NTDB_id=794817 PWP91_RS10625 WP_058223210.1 2130941..2131588(-) (comEA) [Lactococcus lactis strain P-1]

ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT

TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT

CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT

AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA

GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG

AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT

GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA

TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT

TTGAATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-212)

SLBB domain (SLBB)

(70-125)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.559	99.07	0.372

Detailed information NTG0794817