Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HAPS_RS10405 Genome accession   NC_011852
Coordinates   2103246..2103827 (-) Length   193 a.a.
NCBI ID   WP_010786056.1    Uniprot ID   A0A859IE21
Organism   Glaesserella parasuis SH0165     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2098246..2108827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAPS_RS10365 (HAPS_2135) - 2098593..2099231 (+) 639 WP_015940081.1 nitroreductase family protein -
  HAPS_RS10370 (HAPS_2136) - 2099320..2099853 (+) 534 WP_015940082.1 TIGR00645 family protein -
  HAPS_RS10375 (HAPS_2137) - 2099938..2101236 (-) 1299 WP_015940083.1 NAD(P)/FAD-dependent oxidoreductase -
  HAPS_RS10395 (HAPS_2141) greB 2101770..2102249 (-) 480 WP_010786058.1 transcription elongation factor GreB -
  HAPS_RS10400 (HAPS_2142) - 2102249..2103184 (-) 936 WP_015940084.1 KpsF/GutQ family sugar-phosphate isomerase -
  HAPS_RS10405 (HAPS_2143) pilF 2103246..2103827 (-) 582 WP_010786056.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HAPS_RS10410 (HAPS_2144) - 2103875..2104846 (-) 972 WP_012621529.1 IS110 family transposase -
  HAPS_RS10415 (HAPS_2145) - 2105089..2106249 (-) 1161 WP_015940085.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  HAPS_RS10420 (HAPS_2146) - 2106450..2106758 (-) 309 WP_005710405.1 helix-turn-helix domain-containing protein -
  HAPS_RS10425 (HAPS_2147) - 2106864..2107100 (-) 237 WP_015940086.1 hypothetical protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22339.29 Da        Isoelectric Point: 8.4221

>NTDB_id=32586 HAPS_RS10405 WP_010786056.1 2103246..2103827(-) (pilF) [Glaesserella parasuis SH0165]
MTFAKFFRNLTACSVALWLVACTNQTAKVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=32586 HAPS_RS10405 WP_010786056.1 2103246..2103827(-) (pilF) [Glaesserella parasuis SH0165]
ATGACCTTCGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCGTTATGGCTTGTTGCTTGTACAAATCAAACCGC
AAAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCTCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACATGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTGAATAACTACGGTACCTTTCTGTGCAAACAAAAACAGTTCGACAAAGCCTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A859IE21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

97.927

100

0.979
  pilF/pilF2 Haemophilus influenzae Rd KW20

45.251

92.746

0.42

Multiple sequence alignment