Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PPUTGB1_RS25925 Genome accession   NC_010322
Coordinates   5742243..5743505 (-) Length   420 a.a.
NCBI ID   WP_012274632.1    Uniprot ID   A0AAU6YEE0
Organism   Pseudomonas putida GB-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5737243..5748505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPUTGB1_RS28690 - 5738553..5738777 (-) 225 WP_150104963.1 hypothetical protein -
  PPUTGB1_RS25910 (PputGB1_5127) - 5738907..5740511 (-) 1605 WP_012274629.1 AAA family ATPase -
  PPUTGB1_RS25915 (PputGB1_5128) aroB 5740527..5741624 (-) 1098 WP_041166613.1 3-dehydroquinate synthase -
  PPUTGB1_RS25920 (PputGB1_5129) aroK 5741720..5742238 (-) 519 WP_041166614.1 shikimate kinase AroK -
  PPUTGB1_RS25925 (PputGB1_5130) pilQ 5742243..5743505 (-) 1263 WP_012274632.1 type IV pilus secretin PilQ Machinery gene
  PPUTGB1_RS25930 (PputGB1_5131) - 5743435..5744445 (-) 1011 WP_012274633.1 pilus assembly protein PilP -
  PPUTGB1_RS25935 (PputGB1_5132) - 5744442..5744978 (-) 537 WP_012274634.1 PilN domain-containing protein -
  PPUTGB1_RS25940 (PputGB1_5133) pilM 5744975..5745856 (-) 882 WP_012274635.1 type IV pilus biogenesis protein PilM -
  PPUTGB1_RS25945 (PputGB1_5134) - 5746038..5748488 (+) 2451 WP_370739375.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45592.23 Da        Isoelectric Point: 4.9812

>NTDB_id=29933 PPUTGB1_RS25925 WP_012274632.1 5742243..5743505(-) (pilQ) [Pseudomonas putida GB-1]
MKTWMKYLLATLLSVPLASATSYEGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGNVTMRLQDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAADFAAQSAGTRAGQVLEAQLQPLRRELLPIHHAKAADLAELLQSSLVGDGILTGSLSVDERT
NTLVVHMPADRLAELRQLVTQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGAQLRPGKELFVDLGVERAGGSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=29933 PPUTGB1_RS25925 WP_012274632.1 5742243..5743505(-) (pilQ) [Pseudomonas putida GB-1]
ATGAAAACGTGGATGAAATATCTGCTGGCAACCCTGCTGTCGGTTCCGCTGGCGTCGGCGACGTCTTATGAGGGTGAGCC
GCTGTCGTTGAACTTTCAGGATGTAGAGGTGCGCGCGGTACTGCAGGTACTGGCGGACTATGCCGGGGTAAACCTGGTAG
CCAGCGATGCGGTGCAAGGCAACGTCACGATGCGCTTGCAGGACGTGCCGTGGGATCAGGCACTGGACCTGGTGTTGCGC
AGCAAGGGCCTGGCTCGGCGCCAGGAGGGTAATGTGCTGTTGGTGGCGCCTGCCGCCGACTTTGCCGCACAGTCAGCCGG
GACCCGTGCGGGGCAAGTGCTGGAAGCGCAGTTGCAACCTTTGCGTCGGGAACTGCTGCCTATCCACCACGCCAAGGCTG
CCGATTTGGCGGAGTTGCTACAGTCGAGCCTGGTGGGTGACGGTATTCTCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACCCTGGTGGTGCACATGCCTGCCGACCGCCTGGCCGAATTGCGGCAACTGGTGACGCAGTTGGATGTGCCGGTGCG
CCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTACGAGAAGAGCCTGGGGGTGCGCTGGGGCGGGCCGC
TATACGGCGCGCAACTGCGGCCAGGCAAAGAATTGTTCGTCGATCTCGGCGTCGAGCGTGCCGGCGGCAGTATCGGCCTT
GGGTTATTGCGCGGGGACGTGTTGCTGGACCTTGAGCTCAGCGCCATGGAAAAGAGTGGTAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCCGACAAGGAAACGGCGAGGATCCTCAAGGGAACCGAGGTGCCTTACCAGGAAACCAGCCAAA
GCGGTGCGACCTCGGTATCTTTTCGCGAAGCGTCGCTGTCGTTGGAAGTTACCCCGCAGATCACCCCGGACAATAAGGTG
ATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTAAATGCCAAGGTTCGTGTGGCTGATGGCGAAACGATCGTGATTGGTGGCGTTTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.132

100

0.617
  pilQ Acinetobacter baumannii D1279779

41.379

100

0.457
  pilQ Acinetobacter baumannii strain A118

41.379

100

0.457
  comQ Acinetobacter baylyi ADP1

41.907

100

0.45
  pilQ Legionella pneumophila strain ERS1305867

43.675

99.762

0.436
  pilQ Legionella pneumophila strain Lp02

43.675

99.762

0.436
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.745

100

0.421
  pilQ Vibrio cholerae strain A1552

41.745

100

0.421
  pilQ Vibrio campbellii strain DS40M4

40.46

100

0.419

Multiple sequence alignment