Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PPUTGB1_RS25990 Genome accession   NC_010322
Coordinates   5757463..5758461 (-) Length   332 a.a.
NCBI ID   WP_012274645.1    Uniprot ID   -
Organism   Pseudomonas putida GB-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5752463..5763461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPUTGB1_RS25970 (PputGB1_5139) argS 5753665..5755401 (+) 1737 WP_012274641.1 arginine--tRNA ligase -
  PPUTGB1_RS25975 (PputGB1_5140) - 5755403..5756101 (+) 699 WP_012274642.1 SPOR domain-containing protein -
  PPUTGB1_RS25980 (PputGB1_5141) - 5756139..5756558 (-) 420 WP_012274643.1 NINE protein -
  PPUTGB1_RS25985 (PputGB1_5142) - 5756725..5757351 (+) 627 WP_012274644.1 C40 family peptidase -
  PPUTGB1_RS25990 (PputGB1_5143) pilT 5757463..5758461 (-) 999 WP_012274645.1 type IV pilus twitching motility protein PilT Machinery gene
  PPUTGB1_RS25995 (PputGB1_5144) - 5758527..5759213 (+) 687 WP_012274646.1 YggS family pyridoxal phosphate-dependent enzyme -
  PPUTGB1_RS26000 (PputGB1_5145) proC 5759252..5760070 (+) 819 WP_012274647.1 pyrroline-5-carboxylate reductase -
  PPUTGB1_RS26005 (PputGB1_5146) - 5760081..5760671 (+) 591 WP_012274648.1 YggT family protein -
  PPUTGB1_RS26010 (PputGB1_5147) metX 5760760..5761899 (+) 1140 WP_012274649.1 homoserine O-succinyltransferase MetX -
  PPUTGB1_RS26015 (PputGB1_5148) metW 5761907..5762527 (+) 621 WP_012274650.1 methionine biosynthesis protein MetW -
  PPUTGB1_RS26020 (PputGB1_5149) - 5762550..5762984 (+) 435 WP_012274651.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35798.33 Da        Isoelectric Point: 6.0470

>NTDB_id=29934 PPUTGB1_RS25990 WP_012274645.1 5757463..5758461(-) (pilT) [Pseudomonas putida GB-1]
MDVTDLLARAVDAGASDLHLAAGQIPMLRLDGELQGMDLPTLGADALIDGMAAVLDDHQRRQWAHGDELDLALELPALGR
FRLNLFRQLNGPAVTFRLIQGRIATLDELDLADVFQAVAQCRDGLILVGGPTGSGKSSTLATLVDQLNRERALHIITLED
PVEVIHGSQRSLVNQREVGCHSHSFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHARSAASSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRVGGGRVAAREVLVATPAVRNLVREGRMAQLCSVMQAGGVEGMRTMEGAM
RGLKEKGLINDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=29934 PPUTGB1_RS25990 WP_012274645.1 5757463..5758461(-) (pilT) [Pseudomonas putida GB-1]
ATGGACGTGACTGACCTGTTGGCCCGGGCCGTGGATGCGGGGGCTTCGGACCTGCACCTGGCGGCGGGCCAGATACCGAT
GCTGCGTTTGGATGGCGAGTTGCAGGGGATGGATTTGCCGACCTTGGGGGCGGATGCCTTGATCGATGGCATGGCTGCCG
TGCTGGATGACCACCAGCGACGACAGTGGGCCCATGGCGATGAACTGGATCTGGCGCTGGAGTTACCTGCCCTTGGGCGT
TTTCGGCTGAACCTGTTTCGCCAATTGAATGGCCCAGCTGTCACTTTTCGGTTGATTCAGGGGCGAATTGCCACGCTGGA
TGAGCTTGATCTGGCAGATGTGTTTCAAGCTGTTGCCCAATGCCGTGATGGCTTGATCCTGGTGGGCGGGCCGACCGGCA
GTGGCAAGTCCAGCACCCTGGCGACGCTGGTTGATCAACTGAATCGCGAGCGGGCGCTGCACATCATTACCCTGGAGGAC
CCTGTCGAAGTTATCCACGGCAGCCAGCGCAGCCTGGTCAATCAGCGCGAGGTGGGGTGTCATAGCCACAGCTTTGCTCA
GGGGCTACGCAGTGCGCTGCGCCAGGACCCGGATGTGATCATGATCGGTGAGTTGCGTGACCTGGAAACCATTCGCCTGG
CCCTGCGCGCGGCCGAAACCGGGCATCTGGTGCTGGCAACCGTGCATGCACGTTCGGCGGCCAGCAGTATCGACAGGTTG
GTGGAGGTATTCGCTGCCGAGGAAAAGCCGTTGGTGCGGGCGATGTTGGCCGAGTCGCTGCGCCTGGTGGTCGCGCAGGT
GCTGGTCAGGCGTGTGGGTGGTGGACGGGTGGCGGCTCGGGAAGTGCTGGTGGCGACGCCGGCGGTGCGCAATCTGGTTC
GGGAGGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGAGGGGTGGAGGGGATGCGGACGATGGAGGGGGCGATG
CGGGGGTTGAAAGAGAAGGGGCTGATCAACGACCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.606

99.398

0.503
  pilT Acinetobacter baylyi ADP1

49.697

99.398

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.697

99.398

0.494
  pilT Acinetobacter baumannii strain A118

49.394

99.398

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

99.398

0.491
  pilT Vibrio cholerae strain A1552

49.394

99.398

0.491
  pilT Acinetobacter nosocomialis M2

49.394

99.398

0.491
  pilT Acinetobacter baumannii D1279779

49.394

99.398

0.491
  pilT Neisseria meningitidis 8013

48.788

99.398

0.485
  pilT Neisseria gonorrhoeae MS11

48.485

99.398

0.482
  pilT Legionella pneumophila strain Lp02

48.485

99.398

0.482
  pilT Legionella pneumophila strain ERS1305867

48.485

99.398

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.619

99.699

0.455
  pilU Pseudomonas stutzeri DSM 10701

37.576

99.398

0.373
  pilU Vibrio cholerae strain A1552

37.423

98.193

0.367

Multiple sequence alignment