Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BPUM_RS11970 Genome accession   NC_009848
Coordinates   2414363..2416687 (-) Length   774 a.a.
NCBI ID   WP_012010733.1    Uniprot ID   -
Organism   Bacillus pumilus SAFR-032     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2409363..2421687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BPUM_RS11955 (BPUM_2400) dtd 2411012..2411455 (-) 444 WP_003216527.1 D-aminoacyl-tRNA deacylase -
  BPUM_RS11960 (BPUM_2401) - 2411474..2413684 (-) 2211 WP_012010732.1 RelA/SpoT family protein -
  BPUM_RS11965 (BPUM_2402) - 2413827..2414339 (-) 513 WP_003216770.1 adenine phosphoribosyltransferase -
  BPUM_RS11970 (BPUM_2403) recJ 2414363..2416687 (-) 2325 WP_012010733.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BPUM_RS11975 (BPUM_2404) - 2416769..2417122 (-) 354 WP_012010734.1 LapA family protein -
  BPUM_RS18870 - 2417224..2417486 (-) 263 Protein_2386 cation:proton antiporter regulatory subunit -
  BPUM_RS11980 (BPUM_2406) secDF 2417648..2419876 (-) 2229 WP_012010735.1 protein translocase subunit SecDF -
  BPUM_RS11985 (BPUM_2407) - 2419918..2420214 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87396.06 Da        Isoelectric Point: 4.8625

>NTDB_id=29291 BPUM_RS11970 WP_012010733.1 2414363..2416687(-) (recJ) [Bacillus pumilus SAFR-032]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKELSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPTQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRLGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEMGMLSPFGMQN
PKPFIMIEDARLEEIRTIGANQNHIKMSLKDEDKLLDCVGFHQGQLKEEVVTGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKNDWEKTIFTLDPEKYVVVCFDEDTKQEVNIPIHHVHAENVSTFDVEGKYVVFADVPADEHLLRQLLE
NQKPARVYTVFQRKEDHFMSSFPSRDQFKWLYGFLLKRGSFPLKEQGMELAKHRGWTKDTMIFMTKVFFELGFVKIENGV
LSIVRDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=29291 BPUM_RS11970 WP_012010733.1 2414363..2416687(-) (recJ) [Bacillus pumilus SAFR-032]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTCCATGATCCATTTCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGTT
GTCTGCGAAAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTACGGGCCTAATGAACAGGCTTTTCGATATATCA
AAGAGCAAGGCGCATCGCTTGTCATCACGGTCGATACAGGCATTGCAGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCGATTGTCCATCCTAAGCA
GCCAGGTTGTGAGTATCCTTTTAAAGAACTTGCCGGTGTAGGTGTGGCGTTTAAAGTGGCGCATGCACTTCTTGGAAAGC
TGCCAACTCAGCTGTTAGATCTTGCAGCAATTGGGACAATTGCGGATCTTGTGCCCCTTCATGATGAAAATAGATGGCTC
GCTAAAAAAGGGCTTATGCAGCTTCGGCAGTCGAATAGGCTAGGGCTGAAGGCGTTATTGAAAGAAGCGGGCGCTACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTAAATGCTGTTGGACGAATTGAACAGGCAGATC
CAGCTGTCCATCTTCTCATGACAGAAGATAAGGATGAGGCAGAAGAACTCGCTCGTTTTGTACAAGAACTGAATAAAGAA
CGTCAAAAAATTGTGAGTACGATTACAGAAGAAGCCATTCAAATGGTGGAAGAGACAGGTGATGACCAGTCTGCGATTGT
TGTAGCGAAAGCGGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCTTCTAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAGGAGACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
TTAAGCAAATGCCGCGACATTCTGCCGCATTTCGGCGGGCACCCGATGGCTGCGGGTATGACACTTCGAGCGGATGATGT
ACCTTTACTAAGGGAAAGATTAAATCAATATGCGAATGAAACGTTGACAGAGGAAGATTTTATTCCGATTCAGCGGGTCG
ATGCCGTATGTAAAGTAGACGAATTGACGGTTGAAGCGATTGAAGAAATGGGGATGCTGTCACCATTTGGCATGCAAAAC
CCAAAGCCGTTCATTATGATTGAAGATGCGAGACTTGAAGAAATCCGAACAATTGGAGCAAATCAGAATCATATTAAAAT
GTCTCTGAAAGATGAAGATAAGCTGCTTGACTGTGTCGGTTTTCATCAAGGTCAATTAAAAGAAGAAGTTGTCACAGGTA
GCCGCATTTCCGTCATTGGTGAAATGTCCATTAATGAATGGAATAACCGGAAGAAACCTCAGCTCATGCTGAAAGATGCC
CGAGTAGATGAATGGCAACTGTTTGATTTGCGCGGCAAAAATGATTGGGAAAAAACCATCTTCACGCTTGACCCAGAAAA
ATATGTGGTGGTCTGTTTTGACGAAGATACAAAACAGGAAGTCAATATCCCGATTCATCATGTTCATGCAGAAAATGTTT
CAACATTCGATGTAGAAGGCAAGTATGTCGTTTTTGCAGATGTACCTGCAGATGAACACTTGCTAAGGCAGCTGTTAGAA
AATCAAAAGCCAGCGAGAGTGTATACGGTTTTCCAAAGAAAAGAAGATCATTTCATGTCCTCTTTCCCTAGCCGCGATCA
ATTCAAATGGTTGTATGGATTCTTGTTGAAACGAGGAAGTTTCCCTCTCAAGGAACAAGGGATGGAGCTTGCGAAACATA
GAGGCTGGACAAAAGATACAATGATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACGTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHHA1 domain (DHHA1)

(309-437)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.439

100

0.677

Multiple sequence alignment