Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   EL191_RS24645 Genome accession   NZ_LR134290
Coordinates   995356..995541 (+) Length   61 a.a.
NCBI ID   WP_041976804.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina strain NCTC10897     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 990356..1000541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL191_RS04505 (NCTC10897_00905) coaE 990409..991017 (-) 609 WP_041976792.1 dephospho-CoA kinase -
  EL191_RS04510 (NCTC10897_00906) pilD 991287..992153 (-) 867 WP_162184340.1 A24 family peptidase Machinery gene
  EL191_RS04515 (NCTC10897_00907) pilC 992157..993377 (-) 1221 WP_041976797.1 type II secretion system F family protein Machinery gene
  EL191_RS04520 (NCTC10897_00908) pilB 993381..995081 (-) 1701 WP_041976801.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL191_RS24645 (NCTC10897_00909) pilA 995356..995541 (+) 186 WP_041976804.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL191_RS04530 - 995559..996011 (+) 453 WP_126403455.1 hypothetical protein -
  EL191_RS04535 (NCTC10897_00910) - 996094..996903 (+) 810 WP_041976806.1 hypothetical protein -
  EL191_RS04540 (NCTC10897_00911) - 996866..997744 (+) 879 WP_230926001.1 hypothetical protein -
  EL191_RS04545 (NCTC10897_00912) - 997741..998652 (+) 912 WP_041976812.1 ABC transporter ATP-binding protein -
  EL191_RS04550 (NCTC10897_00913) nadC 998761..999609 (-) 849 WP_041976814.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6618.89 Da        Isoelectric Point: 10.5719

>NTDB_id=1120338 EL191_RS24645 WP_041976804.1 995356..995541(+) (pilA) [Ectopseudomonas mendocina strain NCTC10897]
MKSLKKQKGFTLIELMIVIAIIGILAAIAIPQFNDYRQKANDTSAVADVKNGVTSIISSLR

Nucleotide


Download         Length: 186 bp        

>NTDB_id=1120338 EL191_RS24645 WP_041976804.1 995356..995541(+) (pilA) [Ectopseudomonas mendocina strain NCTC10897]
ATGAAGTCTCTTAAGAAGCAAAAGGGTTTTACCCTGATCGAGCTGATGATCGTGATCGCGATCATCGGTATTCTGGCTGC
TATCGCCATTCCTCAGTTTAATGATTATCGTCAAAAAGCGAACGATACTTCGGCGGTTGCTGATGTGAAGAACGGTGTTA
CATCTATTATTTCTTCTCTGCGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

57.895

93.443

0.541

  pilA/pilA1 Eikenella corrodens VA1

75.61

67.213

0.508

  pilA Ralstonia pseudosolanacearum GMI1000

62.5

78.689

0.492

  pilA Vibrio parahaemolyticus RIMD 2210633

63.043

75.41

0.475

  pilA/pilAI Pseudomonas stutzeri DSM 10701

63.043

75.41

0.475

  pilA2 Legionella pneumophila strain ERS1305867

68.293

67.213

0.459

  pilA2 Legionella pneumophila str. Paris

68.293

67.213

0.459

  comP Acinetobacter baylyi ADP1

75.676

60.656

0.459

  pilA Vibrio campbellii strain DS40M4

77.778

59.016

0.459

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

77.778

59.016

0.459

  pilA Acinetobacter baumannii strain A118

58.333

78.689

0.459

  pilA Acinetobacter nosocomialis M2

59.574

77.049

0.459

  pilA/pilAII Pseudomonas stutzeri DSM 10701

54

81.967

0.443

  pilA/pilA4 Thermus thermophilus HB27

54

81.967

0.443

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.102

80.328

0.443

  pilE Neisseria gonorrhoeae strain FA1090

65

65.574

0.426

  pilE Neisseria gonorrhoeae MS11

65

65.574

0.426

  pilA Vibrio cholerae C6706

72.222

59.016

0.426

  pilA Vibrio cholerae strain A1552

72.222

59.016

0.426

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

72.222

59.016

0.426

  pilA Glaesserella parasuis strain SC1401

75.758

54.098

0.41

  pilA Haemophilus influenzae Rd KW20

73.529

55.738

0.41

  pilA Haemophilus influenzae 86-028NP

73.529

55.738

0.41

  pilE Acinetobacter baumannii D1279779

51.111

73.77

0.377

  comF Acinetobacter baylyi ADP1

60.526

62.295

0.377


Multiple sequence alignment