Detailed information NTG1120312

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EL191_RS04510
• Genome accession: NZ_LR134290
• Coordinates: 991287..992153 (-)
• Length: 288 a.a.
• NCBI ID: WP_162184340.1
• Uniprot ID: -
• Organism: Ectopseudomonas mendocina strain NCTC10897
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 986287..997153

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL191_RS04475 (NCTC10897_00899)	-	987368..987541 (-)	174	WP_013714021.1	DUF3094 family protein	-
EL191_RS04480 (NCTC10897_00900)	-	987748..988302 (-)	555	WP_041976784.1	MOSC domain-containing protein	-
EL191_RS04485 (NCTC10897_00901)	-	988299..988928 (-)	630	WP_013714023.1	DUF1780 domain-containing protein	-
EL191_RS04490 (NCTC10897_00902)	-	989137..989826 (+)	690	WP_041976787.1	energy-coupling factor ABC transporter permease	-
EL191_RS04495 (NCTC10897_00903)	-	989932..990147 (+)	216	WP_041976790.1	hypothetical protein	-
EL191_RS04500 (NCTC10897_00904)	yacG	990218..990412 (-)	195	WP_013714026.1	DNA gyrase inhibitor YacG	-
EL191_RS04505 (NCTC10897_00905)	coaE	990409..991017 (-)	609	WP_041976792.1	dephospho-CoA kinase	-
EL191_RS04510 (NCTC10897_00906)	pilD	991287..992153 (-)	867	WP_162184340.1	A24 family peptidase	Machinery gene
EL191_RS04515 (NCTC10897_00907)	pilC	992157..993377 (-)	1221	WP_041976797.1	type II secretion system F family protein	Machinery gene
EL191_RS04520 (NCTC10897_00908)	pilB	993381..995081 (-)	1701	WP_041976801.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EL191_RS24645 (NCTC10897_00909)	pilA	995356..995541 (+)	186	WP_041976804.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
EL191_RS04530	-	995559..996011 (+)	453	WP_126403455.1	hypothetical protein	-
EL191_RS04535 (NCTC10897_00910)	-	996094..996903 (+)	810	WP_041976806.1	hypothetical protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31802.76 Da
• Isoelectric Point: 6.2164

>NTDB_id=1120312 EL191_RS04510 WP_162184340.1 991287..992153(-) (pilD) [Ectopseudomonas mendocina strain NCTC10897]
MALEIMADNLSAFVFICVVFGLLIGSFLNVVVYRLPLMLQRDWQSQAREVLELPAAPTTETFNLILPNSSCPNCGHEIRP
WENIPVISYLFLRGKCSNCKTPISKRYPIVELACGLLSGYVAWHFGFTWQAGAMLVLAWGLLAMSLIDADHQILPDVLVL
PLLWLGLIVNYYGLFTSLEGALWGAIAGYLSLWSVYWLFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVGA
VLGVIMLRMRGSDSSTPIPFGPYLAIAGWIALLWGDVITSNYLKFAGF

Nucleotide

• Length: 867 bp

>NTDB_id=1120312 EL191_RS04510 WP_162184340.1 991287..992153(-) (pilD) [Ectopseudomonas mendocina strain NCTC10897]
ATGGCGCTCGAAATTATGGCCGACAATCTGTCGGCCTTTGTTTTTATCTGTGTAGTTTTTGGTCTGCTAATCGGCAGCTT
CCTCAACGTCGTGGTCTACCGCCTACCACTGATGCTGCAACGCGACTGGCAAAGCCAAGCGCGTGAAGTATTGGAACTAC
CAGCGGCGCCGACAACAGAAACTTTCAACCTGATACTCCCCAACTCCTCATGCCCCAACTGCGGCCATGAGATTCGGCCC
TGGGAAAACATTCCGGTGATCAGCTACCTGTTCCTGCGCGGCAAGTGCTCGAACTGCAAGACGCCGATCAGCAAGCGCTA
CCCCATCGTAGAATTGGCCTGCGGCCTGCTTTCAGGCTATGTCGCCTGGCACTTCGGTTTTACCTGGCAGGCCGGAGCGA
TGCTGGTGTTGGCCTGGGGGCTGCTGGCCATGAGTCTGATCGATGCCGATCACCAGATCCTGCCGGATGTGCTGGTGCTG
CCGCTGCTTTGGCTGGGCTTGATCGTCAATTACTACGGGTTGTTCACCAGCCTGGAAGGTGCCTTGTGGGGGGCGATCGC
CGGTTATCTGAGCCTATGGTCGGTGTACTGGCTATTCAAGCTGATCACCGGCAAGGAAGGCATGGGCTATGGTGACTTCA
AGCTGCTGGCCATGCTCGGCGCCTGGGGGGGCTGGCAGATTCTGCCACTGACCATTCTGCTGTCGTCGCTGGTCGGGGCG
GTGCTCGGGGTAATCATGCTACGCATGCGTGGCAGCGACTCCAGCACGCCGATTCCCTTCGGGCCCTATCTGGCGATAGC
CGGGTGGATCGCCTTGCTTTGGGGTGATGTGATTACTAGCAACTATCTGAAGTTCGCTGGGTTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	52.878	96.528	0.51
pilD	Acinetobacter nosocomialis M2	52.518	96.528	0.507
pilD	Vibrio cholerae strain A1552	52.92	95.139	0.503
pilD	Vibrio campbellii strain DS40M4	52.747	94.792	0.5
pilD	Neisseria gonorrhoeae MS11	49.815	94.097	0.469