Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL191_RS04520 Genome accession   NZ_LR134290
Coordinates   993381..995081 (-) Length   566 a.a.
NCBI ID   WP_041976801.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina strain NCTC10897     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 988381..1000081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL191_RS04490 (NCTC10897_00902) - 989137..989826 (+) 690 WP_041976787.1 energy-coupling factor ABC transporter permease -
  EL191_RS04495 (NCTC10897_00903) - 989932..990147 (+) 216 WP_041976790.1 hypothetical protein -
  EL191_RS04500 (NCTC10897_00904) yacG 990218..990412 (-) 195 WP_013714026.1 DNA gyrase inhibitor YacG -
  EL191_RS04505 (NCTC10897_00905) coaE 990409..991017 (-) 609 WP_041976792.1 dephospho-CoA kinase -
  EL191_RS04510 (NCTC10897_00906) pilD 991287..992153 (-) 867 WP_162184340.1 A24 family peptidase Machinery gene
  EL191_RS04515 (NCTC10897_00907) pilC 992157..993377 (-) 1221 WP_041976797.1 type II secretion system F family protein Machinery gene
  EL191_RS04520 (NCTC10897_00908) pilB 993381..995081 (-) 1701 WP_041976801.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL191_RS24645 (NCTC10897_00909) pilA 995356..995541 (+) 186 WP_041976804.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL191_RS04530 - 995559..996011 (+) 453 WP_126403455.1 hypothetical protein -
  EL191_RS04535 (NCTC10897_00910) - 996094..996903 (+) 810 WP_041976806.1 hypothetical protein -
  EL191_RS04540 (NCTC10897_00911) - 996866..997744 (+) 879 WP_230926001.1 hypothetical protein -
  EL191_RS04545 (NCTC10897_00912) - 997741..998652 (+) 912 WP_041976812.1 ABC transporter ATP-binding protein -
  EL191_RS04550 (NCTC10897_00913) nadC 998761..999609 (-) 849 WP_041976814.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62290.20 Da        Isoelectric Point: 5.3427

>NTDB_id=1120314 EL191_RS04520 WP_041976801.1 993381..995081(-) (pilB) [Ectopseudomonas mendocina strain NCTC10897]
MNDTVIATGLARQLVNAELLDERTASNAQAQAARNKIPLVSYLVQNKLISSRALAEVAAEQFGMPYFDLTAIDKESQPRE
VLSEKLCRQHRVLPLYRRGNKLFIALSDPTNHQALTDVQFSAGLSTEALLVEDDKLGDALERFFDTGNTGMEDLADVDLD
GLDVEAVTDDKDDAGSGGDADDAPVVRFINKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGMLHEVARPPIQLASRLSAR
LKVMAQLDISERRKPQDGRIKMKVSKNKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEGQKELYMTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKHGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSCKKEVELPREALLKEGFPE
SQIGTFKLYAPNGCDNCKGGYKGRVGIYEVVKITPSLQRIIMEEGNSIEIAKQARADGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1120314 EL191_RS04520 WP_041976801.1 993381..995081(-) (pilB) [Ectopseudomonas mendocina strain NCTC10897]
ATGAACGACACCGTGATCGCAACTGGCTTGGCCCGACAACTTGTCAATGCCGAACTGCTCGACGAACGTACAGCCAGCAA
CGCTCAGGCACAGGCTGCCCGTAACAAGATTCCGCTGGTGTCTTATTTGGTACAGAACAAGCTGATAAGCAGTCGCGCGC
TGGCGGAGGTGGCAGCCGAGCAATTTGGCATGCCCTACTTCGACCTGACTGCCATCGACAAGGAAAGCCAGCCTCGCGAG
GTGCTAAGCGAAAAACTCTGTCGGCAACATCGGGTACTACCGCTCTACCGCCGAGGCAACAAGCTGTTTATCGCCCTCTC
CGACCCGACCAATCACCAGGCGCTGACCGATGTTCAATTCAGTGCCGGGCTTAGCACCGAGGCGCTACTGGTCGAGGACG
ACAAGCTGGGCGATGCCTTGGAGCGTTTTTTCGATACTGGCAATACCGGCATGGAGGATTTGGCCGATGTTGACCTGGAT
GGACTGGATGTAGAGGCCGTCACCGACGACAAGGACGATGCTGGCTCGGGCGGCGATGCCGATGATGCGCCAGTGGTGCG
CTTTATCAACAAAATGCTGCTGGATGCGATCAAGGGCGGCTCATCCGACCTGCACTTCGAACCCTACGAGAAAACCTATC
GCGTGCGCTTTCGTACCGACGGTATGTTGCACGAGGTGGCACGCCCGCCAATCCAACTGGCCAGTCGCCTGTCTGCTCGC
CTAAAGGTCATGGCGCAATTGGACATCTCCGAGCGACGCAAGCCGCAGGATGGCCGGATCAAGATGAAAGTATCGAAGAA
CAAGGCCATCGATTTTCGCGTCAACACCCTGCCTACCCTGTGGGGCGAAAAGATCGTGATGCGGATTCTCGACCCCACCA
GCGCCCAGATGGGTATCGATGCCCTGGGTTACGAAGAGGGTCAGAAAGAGCTGTATATGACAGCTCTAAAGCAGCCGCAG
GGAATGATTCTGGTAACCGGCCCCACAGGTTCGGGCAAAACCGTATCACTCTATACCGGGCTGAATATTCTCAATACGCC
GGATATCAATATCTCCACCGCAGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCCAAGC
ATGGCATGGACTTCTCGCAGGCTCTGCGAGCGTTCCTGCGTCAGGATCCAGACGTGATCATGGTCGGTGAGATTCGCGAC
CTGGAAACCGCCGAAATCGCGGTAAAGGCCGCGCAAACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCGGC
GGAAACCCTGACACGTCTACGCAATATGGGCGTACCAGCATTCAATATCGCCACCTCGGTCAACCTGATCATCGCTCAGC
GCCTGGCGCGCAAACTCTGTAGCTGCAAGAAAGAAGTAGAACTGCCGCGCGAGGCTTTACTAAAGGAAGGCTTCCCCGAG
AGCCAGATTGGCACCTTCAAGCTTTACGCCCCCAACGGCTGCGATAATTGCAAAGGCGGCTATAAAGGAAGGGTGGGGAT
TTATGAAGTAGTTAAAATCACGCCCTCGCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGAAATCGCCAAGCAGG
CGCGAGCCGATGGTTTCAACGACCTCCGTACTTCAGGGCTGCTCAAGGCGATGCAGGGCATCACCAGCCTGGAAGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.68

99.47

0.594
  pilB Acinetobacter baylyi ADP1

58.719

99.293

0.583
  pilB Legionella pneumophila strain ERS1305867

54.352

99.47

0.541
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilF Neisseria gonorrhoeae MS11

49.824

100

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

51.577

95.23

0.491
  pilB Vibrio campbellii strain DS40M4

48.929

98.94

0.484
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.286

0.373
  pilF Thermus thermophilus HB27

39.623

93.64

0.371

Multiple sequence alignment