Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negative regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0001	vfu_A02134 (ihfA)	T6SS00529 ; T6SS00530	protein	Transcription regulator	Vibrio furnissii NCTC 11218	chromosome (NC_016602)	2226951..2227244 (-)	490864172	tssD2_a promoter (↓); VflT6SS2 major cluster promoter (↓)
REG0002	vfu_A02422 (ihfB)	T6SS00529 ; T6SS00530	protein	Transcription regulator	Vibrio furnissii NCTC 11218	chromosome (NC_016602)	2516092..2516376 (-)	490864456	tssD2_a promoter (↓); VflT6SS2 major cluster promoter (↓)
REG0063	PA4778 (CueR)	T6SS00004	protein	Transcription regulator	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	5366257..5366655 (+)	15599972	tssA2 (-173 to -140 relative to the ATG start codon) (↓); hcp2 (-208 to -159 relative to the ATG start codon) (↓)
REG0114	VP0820 (ToxR)	T6SS00043	protein	Transcription regulator	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	847892..848770 (-)	28897594	VP1400 promotor (↑); VP1409 promotor (↑)
REG0115	VP0350 (CalR/LeuO)	T6SS00044	protein	Transcription regulator	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	344763..345722 (+)	28897124	VPA1027 (hcp2) promotor (↓); VPA1043 (Fha) promotor (↓); VP1133 (H-NS) (↓)
REG0126	BMAA1517	T6SS00070	protein	Transcription regulator	Burkholderia mallei ATCC 23344	chromosome (NC_006349)	1646532..1647593 (-)	499517952	Hcp1 (BMAA0742) expression (↓)
REG0245	ETAE_3098 (PepA)	T6SS00372	protein	Transcription regulator	Edwardsiella tarda EIB202	chromosome (NC_013508)	3260322..3261833 (+)	502612947	ETAE_0886 (esrB) promoter (↑)
REG0247	ETAE_1505 (H-NS)	T6SS00372	protein	Transcription regulator	Edwardsiella tarda EIB202	chromosome (NC_013508)	1615099..1615506 (-)	505274277	ETAE_2430 (evpB) (↑); evpC promotor (↑)
REG0248	EC042_RS26480 (aggR)	T6SS00464	protein	Transcription regulator	Escherichia coli 042	plasmid (NC_017627)	41080..41877 (-)	446692110	EC042_RS26480 (aggR) promoter (↓); EC042_RS26480 (aggR) promoter (↓)
REG0249	EC042_RS18855 (FIS)	T6SS00464	protein	Transcription regulator	Escherichia coli 042	chromosome (NC_017626)	3772795..3773091 (+)	446385050	EC042_RS26480 (aggR) promoter (↓)
REG0254	HMPREF1171_RS04760	T6SS00788	protein	Transcription regulator	Aeromonas dhakensis strain SSU	chromosome (NZ_JH815591)	1041366..1042904 (+)	WP_005299999.1	hcp expression (↓)
REG0268	rpoN	T6SS00793	protein	Transcription regulator	Vibrio alginolyticus strain EPJS	chromosome (FJ790776)	-	-	T6SS1 transcript (↓)
REG0269	VasH	T6SS00793	protein	Transcription regulator	Vibrio alginolyticus strain EPJS	chromosome (FJ790776)	-	-	T6SS1 transcript (↓)
REG0270	CXG97_03740 (Fur)	T6SS00904	protein	Transcription regulator	Escherichia coli strain ExPEC XM	chromosome (NZ_CP025328)	729474..729920 (-)	446053847	T6SS1 (TssB) promotor (↑)
REG0276	V12G01_19736 (TfoX)	T6SS00939	protein	Transcription regulator	Vibrio alginolyticus 12G01	chromosome (AAPS01000019)	47689..48276 (-)	91189146	T6SS2 transcripts (↓)
REG0277	V12G01_20658 (TfoY)	T6SS00939	protein	Transcription regulator	Vibrio alginolyticus 12G01	chromosome (AAPS01000039)	21665..22255 (+)	91188194	T6SS transcripts (↓)
REG0278	V12G01_20201 (H-NS)	T6SS00939	protein	Transcription regulator	Vibrio alginolyticus 12G01	chromosome (AAPS01000015)	63783..64190 (+)	91189474	VaT6SS1 transcripts (↑)
REG0294	VCA1552_RS04890 (ihfA)	T6SS01112	protein	Transcription regulator	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	1028170..1028466 (+)	447152004	Hcp secretion (↓)
REG0295	VCA1552_RS08565 (ihfB)	T6SS01112	protein	Transcription regulator	Vibrio cholerae strain A1552	chromosome (NZ_CP024867)	1839353..1839631 (-)	446089486	Hcp secretion (↓)
REG0320	VC_A0122	T6SS00002	protein	Transcription regulator	Vibrio cholerae O1 biovar El Tor str. N16961	chromosome (NC_002506)	137379..137621 (+)	9657509	hcp-2 expression (↓)
REG0329	VPBB_RS05425 (H-NS)	T6SS00719	protein	Transcription regulator	Vibrio parahaemolyticus BB22OP	chromosome (NC_019955)	1170475..1170882 (-)	505109423	VgrG1 expression and secretion (↑)
REG0364	Y113_RS11250 (RpoS)	T6SS00791	protein	Transcription regulator	Edwardsiella tarda PPD130/91	chromosome (NZ_JACQ01000026)	9122..10108 (-)	502612732	EvpQ transcription and protein level (↑)
REG0372	BMUL_RS00795 (hns)	-	protein	Transcription regulator	Burkholderia multivorans ATCC 17616	chromosome (NC_010084)	179094..179390 (+)	WP_006401402.1	T6SS (↑)