Detailed information of regulator


Summary

Regulator ID REG0002 experimental
Regulator type protein
Regulatory pathway (from literatures) Transcription regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00529 insolico (Type i5); T6SS00530 insolico (Type i1)
Strain Vibrio furnissii NCTC 11218
Replicon chromosome
Sequence Protein sequence (95 a.a.); Nucleotide sequence (285 bp)
Description IHF Positively Regulates V. fluvialis VflT6SS and there are direct binding of IHF on tssD2_a promoter and VflT6SS2 major cluster promoter.GeneID of Ihf and primer designation are based on the sequence of V.furnissii NCTC11218.
Reference
External database links

Locus tag (Gene) vfu_A02422 (ihfB)
Coordinates (Strand) 2516092..2516376 (-)
NCBI ID 490864456
RefSeq NC_016602
Uniprot ID A0A0Q2RNT5_VIBFU
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
tssD2_a promoter↓TAACTAATTGATT The IHF binding sites and the conserved bases in E. coli are indicated with red
VflT6SS2 major cluster promoter↓-
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
Bac_DNA_bindingPF00216.236.2e-301..91
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-94MTKSELIERLCAEQTHLSAKEIEDAVKDILEHMANTLEAGDRIEIRGFGSFSLHYREPRVGRNPKTGEKVELEGKFVPHFKPGKELRERVNENI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9896--


  Protein sequence of regulator: 95 a.a.    .

>REG0002 NC_016602:c2516376-2516092 [Vibrio furnissii NCTC 11218]
MTKSELIERLCAEQTHLSAKEIEDAVKDILEHMANTLEAGDRIEIRGFGSFSLHYREPRVGRNPKTGEKVELEGKFVPHF
KPGKELRERVNENI*

  Nucleotide sequence of regulator: 285 bp    .

>REG0002 NC_016602:c2516376-2516092 [Vibrio furnissii NCTC 11218]
ATGACTAAGTCTGAATTGATTGAAAGACTCTGTGCTGAGCAAACACATTTGTCTGCAAAAGAGATCGAAGACGCGGTAAA
AGATATTCTTGAGCACATGGCAAACACCCTAGAGGCAGGAGACCGTATCGAAATTCGTGGATTCGGCAGTTTTTCCCTGC
ACTATCGTGAGCCAAGAGTCGGGCGCAACCCGAAAACTGGTGAGAAAGTAGAGTTGGAAGGTAAATTCGTTCCACACTTC
AAACCAGGCAAAGAACTCCGTGAACGTGTAAACGAAAATATCTGA