Experimental investigation has been performed.
Bioinformatics investigation has been performed

T6SS ID	Type	Organism	Replicon (Accession)	Location	Function
T6SS01168	i1	Vibrio cholerae C6706	chromosome (NZ_CP046845)	296505..315674	anti-bacterial; anti-eukaryotic

Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0021	GPY04_RS11195 (CytR)	T6SS01168	protein	Unknown	Vibrio cholerae C6706	chromosome (NZ_CP046844)	2449479..2450486 (-)	446146597	Unknown (↓)
REG0022	GPY04_RS03590 (TfoX)	T6SS01168	protein	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	777344..777952 (+)	487807468	Unknown (↓)
REG0023	GPY04_RS00870 (HapR)	T6SS01168	protein	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	164935..165546 (-)	446262243	Unknown (↓)
REG0024	GPY04_RS13335/VC0070 (tsrA)	T6SS01168	protein	Global regulator	Vibrio cholerae C6706	chromosome (NZ_CP046844)	2888303..2888587 (+)	445982516	T6SS transcripts (↑)
REG0025	Qrr1 quorum regulatory RNA	T6SS01168	ncRNA	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	-	-	VCA0107 mRNA (↑)
REG0026	Qrr2 quorum regulatory RNA	T6SS01168	ncRNA	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	-	-	VCA0107 mRNA (↑)
REG0027	Qrr3 quorum regulatory RNA	T6SS01168	ncRNA	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	-	-	VCA0107 mRNA (↑)
REG0028	Qrr4 quorum regulatory RNA	T6SS01168	ncRNA	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	-	-	VCA0107 mRNA (↑)
REG0030	GPY04_RS13825 (QstR)	T6SS01168	protein	Global regulator	Vibrio cholerae C6706	chromosome (NZ_CP046844)	2994333..2994977 (-)	447111060	Unknown (↓)
REG0033	GPY04_RS01040 (ChiS)	T6SS01168	protein	ChiS-induced regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	210606..213995 (-)	446410424	Necessory for TfoX/TfoR (↓)
REG0034	GPY04_RS03200 (chiA2)	T6SS01168	protein	ChiS-induced regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	692014..695280 (+)	487807405	GPY04_RS01040 (ChiS) (↓)
REG0035	GPY04_RS07700 (chiA1)	T6SS01168	protein	ChiS-induced regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046844)	1709613..1712153 (-)	446755770	GPY04_RS01040 (ChiS) (↓)
REG0029	GPY04_RS15835 (luxO)	T6SS01168	protein	QS regulatory pathway	Vibrio cholerae C6706	chromosome (NZ_CP046845)	273631..274983 (-)	446367907	VC0070 (tsrA) (↑); GPY04_RS00870 (hapR) (↑)

Experimental investigation has been performed.
Bioinformatics investigation has been performed

Effector ID	Locus tag (Gene)	Classification	Cognate immunity protein	Related T6SS	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID
EFF01818	GPY04_RS04830 (TleV1)	Tle	IMU00356	T6SS01168	Vibrio cholerae C6706	chromosome (NZ_CP046844)	1067605..1069530 (+)	446298981
EFF01819	GPY04_RS04820 (VgrG-1)	-	-	T6SS01168	Vibrio cholerae C6706	chromosome (NZ_CP046844)	1063272..1066763 (+)	446134261
EFF01820	GPY04_RS16385 (VasX)	Tme	IMU00357	T6SS01168	Vibrio cholerae C6706	chromosome (NZ_CP046845)	405867..409124 (-)	445992499
EFF01821	GPY04_RS15920	-	IMU00358	T6SS01168	Vibrio cholerae C6706	chromosome (NZ_CP046845)	291886..294939 (-)	446035440

Experimental investigation has been performed.
Bioinformatics investigation has been performed

Immunity protein ID	Locus tag (Gene)	Related T6SS	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Cognate effector
IMU00356	GPY04_RS04835 (TsiV1)	T6SS01168	Vibrio cholerae C6706	chromosome (NZ_CP046844)	1069527..1070270 (+)	446694188	EFF01819
IMU00357	GPY04_RS16380 (TsiV2)	T6SS01168	Vibrio cholerae C6706	chromosome (NZ_CP046845)	405138..405866 (-)	446826823	EFF01821
IMU00358	GPY04_RS15915 (TsiV3)	T6SS01168	Vibrio cholerae C6706	chromosome (NZ_CP046845)	291521..291889 (-)	446984039	EFF01821

Experimental investigation has been performed.
Bioinformatics investigation has been performed

ID	Locus tag (Gene)	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Response to T6SS attack	Known or putative function

Experimental investigation has been performed.
Bioinformatics investigation has been performed

Accessory protein ID	Locus tag (Gene)	Related T6SS	Type	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Related structure protein	Related effector