Detailed information of regulator


Summary

Regulator ID REG0024 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS01168 experimental (Type i1)
Strain Vibrio cholerae C6706
Replicon chromosome
Sequence Protein sequence (95 a.a.); Nucleotide sequence (285 bp)
Description Deletion of luxO or tsrA alone each caused a small but significant increase in transcription of hcp1 and the T6SS structural gene VCA0107, and simultaneous deletion of both genes (ΔluxO tsrA) caused a further increase in their expression.; Secretion of Hcp occurred in strains carrying both WT hapR and the hapR* mutation and, importantly, Qrr4 functioned equally well to repress Hcp secretion in both strains show that, in addition to functioning through HapR, the Qrr sRNAs also repress T6SS via a HapR-independent mechanism.
Reference
External database links

Locus tag (Gene) GPY04_RS13335/VC0070 (tsrA)
Coordinates (Strand) 2888303..2888587 (+)
NCBI ID 445982516
RefSeq NZ_CP046844
Uniprot ID Q9KVR9_VIBCH, A0A0X1L4V1_VIBCO, A0A0H3AMG6_VIBC3, D7HGY5_VIBCL, A0A0K9UN17_VIBCL, A0A0H3Q5F4_VIBCL, C3LPR2_VIBCM, A0A1X1LD77_VIBCL
KEGG ID vch:VC0070, vcm:VCM66_0070, vcx:VAA049_62, vcz:VAB027_62, vcq:EN18_17180, vco:VC0395_A2444
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS transcripts ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-94 MSLTTYEMARILEQLDESPEKVMYGKLLNELGNQSSERIRSAAKQVPLTVLRDLVYQFQQVIESRKGEQVETMAKELAKQGISAEELLNYLQKR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9967 - -


  Protein sequence of regulator: 95 a.a.    .

>REG0024 NZ_CP046844:2888303-2888587 [Vibrio cholerae C6706]
MSLTTYEMARILEQLDESPEKVMYGKLLNELGNQSSERIRSAAKQVPLTVLRDLVYQFQQVIESRKGEQVETMAKELAKQ
GISAEELLNYLQKR*

  Nucleotide sequence of regulator: 285 bp    .

>REG0024 NZ_CP046844:2888303-2888587 [Vibrio cholerae C6706]
ATGTCTCTAACCACCTATGAAATGGCTCGCATTCTGGAACAACTTGATGAATCTCCAGAAAAGGTGATGTATGGCAAGTT
GCTTAATGAATTAGGCAATCAAAGCAGTGAACGTATTCGCAGTGCGGCCAAGCAAGTGCCACTGACCGTGCTACGCGATC
TGGTTTACCAGTTCCAGCAAGTGATCGAGTCACGCAAGGGCGAGCAAGTCGAAACCATGGCTAAAGAACTCGCTAAACAG
GGCATCTCTGCGGAAGAGCTTCTGAATTACTTACAAAAACGCTAA