Detailed information of regulator


Summary

Regulator ID REG0034 experimental
Regulator type protein
Regulatory pathway (from literatures) ChiS-induced regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS01168 experimental (Type i1)
Strain Vibrio cholerae C6706
Replicon chromosome
Sequence Protein sequence (1089 a.a.); Nucleotide sequence (3267 bp)
Description ChiS-induced expression of T6SS genes was initiated by GlcNAc2 when released by chitinases(chiA2) from chitin.
Reference
External database links

Locus tag (Gene) GPY04_RS03200 (chiA2)
Coordinates (Strand) 692014..695280 (+)
NCBI ID 487807405
RefSeq NZ_CP046844
Uniprot ID Q9KT32_VIBCH, A0A0X1KVM9_VIBCO, A0A2V4NSL7_VIBCL
KEGG ID vch:VC1073
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
GPY04_RS01040 (ChiS) ↓ 446410424
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Glyco_hydro_18 PF00704.30 3.5e-80 386..832
ChiC PF06483.13 8.1e-61 841..1008
Big_7 PF17957.3 1.1e-13 268..336
Big_7 PF17957.3 1.7e-10 168..233
CBM_5_12_2 PF14600.8 4.8e-08 1050..1085
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 MFNKISLEIMKR
Transmembrane helix 13-35 SALTLMPTVLALAVGMAMPAVQA
Outside 36-1088 AINSDASVVGTESQWWNTYKVTLTNNGNQPVELRDASIAFDTNLSLSTPSWSAQGISYPSMSFSSNAQGSVFSNRLTLSFDQGSWVKTQLLPGASIDLTLGVSGVLELSLLQSTIALETDGEVEPGEPEISLELASPVQGAEFIEGQTVAIVANVTATNTTVKTVTFLVDGEQIALLEQAPFQASWTAAGEGAHSIKAIVEDASGLLKEQAVRITVKAEEIDPPVEPEVPVAPVIELTNPRNGQSVFLGKVTTLAANATDENNDLTAVEFLVNGESIGRVTQAPYQLAWTPVALGQYTIEAIAYDAAGHQTQTPMVTVNAKEMGTGNLSCDIKQIYREDGTECMGDDHPRRIIGYYTSWRTGKNGLPAYLAGDLPWEKLTHINYAFASINKSDFSMQVDDSATKMTWENVPGAEMDPSLPYQGHFNLLSKFKKQYPDVKTLISVGGWAETGGFYPMTTDLASCSVNMEGIKAFNKSAVDFIRQYDFDGVDIDYEYPSSMKDSGNPVDFEQSNKCRGQLWDNYMVMMTELRKALDKAGEEDGRRYMLTIASPSSAYLLRGMQDFAMQDVLDYVNIMSYDLHGTWNEFVGPQAALFDDGKDAELAKWGVYTTAEYQGIGYLNQAWTHHFFRGAFKPSQINMGIPYYTRGWQGVSGGDKGLWGRAVEPNQSSCPEGTTVCGWGAEGTDNIWHDVDANGNEIKAGVVPMWHAMNLMHAEKLGIDGMPSYGPAWGMDPNNPKHLIEGKYERVWSQELQTAWLWNDTKKVFLSIEDKDSLKPKLDYIVDNGLGGMMIWEMAGDYSYDAVKREYVIGSDMTSYAHEVFAAAKPMDIRHNDLPQPDSVIDVTVSTTEWPEGDSNYPINPKLVFTNHSNVAIPGGSTIEFLMPTSSGDIVKDWSGAGLKVIESGHTGSNFSVNGEKKLFHKVAVTLRSTETIPAGGEYSVDMVYYTPVSGVANGVRFIVGNETVGLKKDFPTLPAFEGATDGGNGNEGGAGQSCADAGINPNGLKVYPEFPRGDHAAGGDQLIHNGSVWQANWWTNSTPSSADASWKAVCKI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9551 pos: 35-36. VQA-AI. MFNKISLEIMKRSALTLMPTVLALAVGMAMPAVQA


  Protein sequence of regulator: 1089 a.a.    .

>REG0034 NZ_CP046844:692014-695280 [Vibrio cholerae C6706]
MFNKISLEIMKRSALTLMPTVLALAVGMAMPAVQAAINSDASVVGTESQWWNTYKVTLTNNGNQPVELRDASIAFDTNLS
LSTPSWSAQGISYPSMSFSSNAQGSVFSNRLTLSFDQGSWVKTQLLPGASIDLTLGVSGVLELSLLQSTIALETDGEVEP
GEPEISLELASPVQGAEFIEGQTVAIVANVTATNTTVKTVTFLVDGEQIALLEQAPFQASWTAAGEGAHSIKAIVEDASG
LLKEQAVRITVKAEEIDPPVEPEVPVAPVIELTNPRNGQSVFLGKVTTLAANATDENNDLTAVEFLVNGESIGRVTQAPY
QLAWTPVALGQYTIEAIAYDAAGHQTQTPMVTVNAKEMGTGNLSCDIKQIYREDGTECMGDDHPRRIIGYYTSWRTGKNG
LPAYLAGDLPWEKLTHINYAFASINKSDFSMQVDDSATKMTWENVPGAEMDPSLPYQGHFNLLSKFKKQYPDVKTLISVG
GWAETGGFYPMTTDLASCSVNMEGIKAFNKSAVDFIRQYDFDGVDIDYEYPSSMKDSGNPVDFEQSNKCRGQLWDNYMVM
MTELRKALDKAGEEDGRRYMLTIASPSSAYLLRGMQDFAMQDVLDYVNIMSYDLHGTWNEFVGPQAALFDDGKDAELAKW
GVYTTAEYQGIGYLNQAWTHHFFRGAFKPSQINMGIPYYTRGWQGVSGGDKGLWGRAVEPNQSSCPEGTTVCGWGAEGTD
NIWHDVDANGNEIKAGVVPMWHAMNLMHAEKLGIDGMPSYGPAWGMDPNNPKHLIEGKYERVWSQELQTAWLWNDTKKVF
LSIEDKDSLKPKLDYIVDNGLGGMMIWEMAGDYSYDAVKREYVIGSDMTSYAHEVFAAAKPMDIRHNDLPQPDSVIDVTV
STTEWPEGDSNYPINPKLVFTNHSNVAIPGGSTIEFLMPTSSGDIVKDWSGAGLKVIESGHTGSNFSVNGEKKLFHKVAV
TLRSTETIPAGGEYSVDMVYYTPVSGVANGVRFIVGNETVGLKKDFPTLPAFEGATDGGNGNEGGAGQSCADAGINPNGL
KVYPEFPRGDHAAGGDQLIHNGSVWQANWWTNSTPSSADASWKAVCKI*

  Nucleotide sequence of regulator: 3267 bp    .

>REG0034 NZ_CP046844:692014-695280 [Vibrio cholerae C6706]
TTGTTCAATAAAATAAGTCTCGAAATAATGAAAAGATCAGCATTAACTTTAATGCCGACCGTTCTTGCATTAGCAGTGGG
AATGGCGATGCCTGCAGTGCAGGCGGCAATCAATAGTGACGCTTCTGTGGTTGGTACGGAAAGCCAGTGGTGGAATACCT
ACAAAGTGACGCTCACTAACAACGGCAATCAGCCAGTTGAACTGCGTGATGCGAGCATTGCGTTTGATACCAACCTTTCA
CTTTCAACACCTTCTTGGTCAGCACAAGGCATTTCTTACCCAAGCATGAGCTTTAGCAGCAATGCGCAAGGTTCAGTGTT
TAGCAATCGCTTAACTCTGAGCTTTGATCAAGGCAGTTGGGTAAAAACGCAACTGCTACCGGGGGCAAGCATTGATCTTA
CCTTAGGTGTCAGTGGTGTGCTCGAGCTGAGCCTGCTGCAAAGCACGATCGCTCTGGAAACCGATGGTGAAGTGGAGCCG
GGCGAACCAGAAATTTCGCTAGAGCTGGCTTCACCCGTTCAAGGTGCAGAGTTCATTGAAGGCCAGACCGTTGCGATTGT
GGCTAACGTTACTGCAACCAACACAACAGTAAAAACTGTCACGTTCTTGGTGGATGGTGAGCAGATTGCGTTGCTTGAGC
AAGCGCCATTTCAAGCTAGCTGGACTGCGGCTGGTGAGGGGGCTCACTCCATCAAAGCCATCGTGGAAGATGCTTCTGGT
TTGCTGAAAGAGCAAGCGGTACGTATTACCGTCAAAGCCGAAGAAATTGATCCACCGGTTGAGCCAGAAGTACCCGTTGC
ACCTGTGATTGAGTTGACCAATCCACGTAACGGACAAAGCGTATTCTTGGGCAAAGTCACCACACTGGCTGCGAATGCGA
CAGACGAAAATAATGATCTCACCGCAGTTGAGTTTTTGGTGAATGGCGAATCGATTGGCCGTGTTACTCAGGCTCCATAT
CAACTGGCTTGGACTCCGGTAGCTTTGGGTCAATACACCATCGAAGCGATCGCTTACGATGCAGCAGGTCATCAAACGCA
AACCCCAATGGTGACAGTGAACGCCAAAGAAATGGGCACGGGCAACCTAAGTTGTGACATCAAACAGATCTATCGTGAAG
ATGGCACTGAGTGTATGGGTGACGATCACCCGCGCCGTATCATCGGTTATTACACCTCATGGCGTACTGGTAAAAACGGT
CTGCCAGCGTACCTCGCCGGCGATCTGCCTTGGGAAAAACTGACCCACATTAACTACGCGTTCGCCAGCATCAACAAGTC
TGACTTCAGCATGCAAGTGGATGATTCCGCGACCAAGATGACGTGGGAAAATGTACCGGGTGCCGAGATGGATCCTTCAC
TGCCATACCAAGGTCACTTCAACCTGCTGAGCAAGTTTAAGAAACAGTACCCCGATGTGAAAACGCTGATTTCTGTCGGT
GGTTGGGCGGAAACCGGTGGTTTCTATCCAATGACGACCGATCTGGCTTCTTGTTCAGTGAACATGGAAGGCATCAAGGC
GTTCAACAAATCGGCGGTCGATTTCATCCGTCAATACGATTTTGATGGCGTGGATATCGACTACGAATACCCATCATCAA
TGAAAGATTCCGGTAATCCGGTCGATTTCGAGCAGTCGAACAAGTGTCGTGGCCAGTTGTGGGATAACTACATGGTGATG
ATGACCGAACTGCGCAAAGCGCTGGATAAAGCGGGCGAAGAAGATGGCCGTCGTTATATGCTGACTATCGCGTCACCTTC
ATCAGCTTACCTGCTGCGTGGTATGCAAGACTTCGCGATGCAAGATGTACTGGATTACGTCAACATCATGTCTTACGACC
TGCATGGTACGTGGAACGAGTTTGTCGGCCCACAAGCCGCACTGTTTGATGATGGTAAAGATGCTGAGCTGGCCAAATGG
GGCGTGTACACGACTGCAGAATATCAAGGCATTGGCTACTTAAACCAAGCGTGGACGCACCACTTCTTCCGTGGCGCCTT
TAAACCAAGCCAAATCAACATGGGGATTCCTTACTACACCCGTGGCTGGCAAGGCGTGAGCGGCGGCGATAAAGGTCTAT
GGGGTCGTGCGGTTGAGCCAAACCAATCGTCATGTCCAGAAGGCACCACTGTTTGTGGTTGGGGCGCGGAAGGTACCGAT
AACATCTGGCATGATGTGGATGCCAACGGTAACGAAATCAAAGCCGGTGTGGTACCCATGTGGCACGCGATGAACCTGAT
GCACGCTGAAAAATTGGGTATTGACGGTATGCCAAGTTACGGCCCAGCGTGGGGTATGGATCCAAACAACCCGAAACACC
TGATTGAAGGCAAATACGAGCGTGTGTGGAGTCAAGAGCTGCAAACGGCATGGCTTTGGAACGACACCAAAAAAGTATTC
CTGAGCATTGAAGATAAAGACTCACTGAAGCCAAAACTGGATTACATCGTCGATAACGGTTTAGGCGGCATGATGATTTG
GGAGATGGCGGGTGACTACTCGTATGATGCGGTGAAACGCGAATACGTGATCGGTTCTGACATGACTTCTTATGCCCATG
AAGTGTTTGCCGCGGCGAAGCCGATGGATATTCGTCATAACGATCTGCCACAACCAGACTCGGTGATTGACGTTACGGTA
TCGACCACAGAATGGCCAGAAGGCGATAGCAACTACCCAATCAACCCGAAATTGGTGTTTACCAACCACTCAAATGTGGC
GATTCCGGGTGGTTCTACGATTGAGTTCCTGATGCCAACGTCAAGCGGTGATATCGTCAAAGATTGGAGCGGTGCTGGTC
TGAAAGTGATTGAAAGCGGTCATACCGGCAGCAACTTCTCGGTGAATGGTGAGAAGAAACTGTTCCACAAAGTGGCCGTG
ACACTGCGCTCAACCGAAACGATCCCTGCGGGCGGTGAGTACAGCGTCGATATGGTGTACTACACCCCAGTCTCTGGCGT
GGCTAACGGCGTGCGCTTTATCGTGGGTAACGAAACGGTCGGCTTGAAGAAAGACTTCCCAACATTGCCAGCGTTTGAAG
GTGCCACCGATGGCGGCAATGGGAACGAAGGCGGTGCGGGTCAATCTTGTGCCGATGCCGGCATCAACCCTAATGGCTTG
AAAGTGTACCCAGAGTTTCCACGTGGCGACCACGCGGCAGGCGGTGACCAACTGATCCACAATGGTTCTGTATGGCAAGC
CAACTGGTGGACGAACAGTACACCATCCAGCGCCGATGCATCATGGAAAGCGGTGTGCAAAATCTAA