Detailed information of effector


Summary

Effector ID EFF01818 experimental
Effector type Tle
Cognate immunity protein IMU00356 experimental
Related T6SS (Type) T6SS01168 experimental (Type i1)
Strain Vibrio cholerae C6706
Replicon chromosome
Sequence Protein sequence (642 a.a.); Nucleotide sequence (1926 bp)
Description TleV1 is toxic to E. coli cells and can be used in intra-species T6SS-mediated competition.TleV1 was most cytotoxic when delivered to the periplasm, but also had moderate toxicity in the cytoplasm
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) GPY04_RS04830 (TleV1)
Coordinates (Strand) 1067605..1069530 (+)
NCBI ID 446298981
RefSeq NZ_CP046844
Uniprot ID TSEL_VIBCH, A0A0X1KWD5_VIBCO, A0A0K9UZ72_VIBCL, A0A0H3PXY5_VIBCL, C3LMB3_VIBCM, A0A023PTD0_VIBCL
KEGG ID vch:VC1418, vcq:EN18_10310, vcm:VCM66_1373
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Lipase_3 PF01764.27 5e-18 320..433
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-641 MDSFNYCVQCNPEENWLELEFRSENDEPIDGLLVTITNQSAPSNTYTQTTSSGKVLFGKIAAGEWRASVSQASLLTEVEKYASRKEGQESPVKKRAAAELDAADKDTKQYRFTTIGDFWDEAPKDEFLQKQHKGIDVNASAEKAGFRLSHNQTYVFEIKALRSYMPVIIDTDEFNLVNSYTFALLSKLAYATNDFNRDDGKTIDNQGAISTVISQLKRKERPTYSGDLQAKWLLEEIPYSKALSAQYYAEDDVGSEGYIIFNDELAIIGVRGTEPYFQSKKPPVDNTKFKIIKAASGMAAVIADKIESATDSPGMKDLIITDLDAAQIAPEEFGGTYVHRGFYQYTMALLSLMEKDLGLHKIKKFYCCGHSLGGAGALLISALIKDSYHPPVLRLYTYGMPRVGTRSFVERYQNILHYRHVNNHDLVPQIPTVWMNTDVSEGFHVLDVFKSRVDLMRKMLTDDDDDNYQHHGHLSQLLTYNSNNQVLLTPKQTQVTMLDLANLATNDSVAMVDGLSDASIVEHGMEQYIPNLFEQLTALSDESLMVHYQRAISALEQEIATLQQSYLTVKQAWIESIGNGTPTMNIGRLMSEMHSINKLIENRNKIRGELRQIVSDPQRMPATKFLISQQTLPDEIKVQIR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9852 - -


  Protein sequence: 642 a.a.    .

>EFF01818 NZ_CP046844:1067605-1069530 Type VI Lipase Effector (Tle) [Vibrio cholerae C6706]
MDSFNYCVQCNPEENWLELEFRSENDEPIDGLLVTITNQSAPSNTYTQTTSSGKVLFGKIAAGEWRASVSQASLLTEVEK
YASRKEGQESPVKKRAAAELDAADKDTKQYRFTTIGDFWDEAPKDEFLQKQHKGIDVNASAEKAGFRLSHNQTYVFEIKA
LRSYMPVIIDTDEFNLVNSYTFALLSKLAYATNDFNRDDGKTIDNQGAISTVISQLKRKERPTYSGDLQAKWLLEEIPYS
KALSAQYYAEDDVGSEGYIIFNDELAIIGVRGTEPYFQSKKPPVDNTKFKIIKAASGMAAVIADKIESATDSPGMKDLII
TDLDAAQIAPEEFGGTYVHRGFYQYTMALLSLMEKDLGLHKIKKFYCCGHSLGGAGALLISALIKDSYHPPVLRLYTYGM
PRVGTRSFVERYQNILHYRHVNNHDLVPQIPTVWMNTDVSEGFHVLDVFKSRVDLMRKMLTDDDDDNYQHHGHLSQLLTY
NSNNQVLLTPKQTQVTMLDLANLATNDSVAMVDGLSDASIVEHGMEQYIPNLFEQLTALSDESLMVHYQRAISALEQEIA
TLQQSYLTVKQAWIESIGNGTPTMNIGRLMSEMHSINKLIENRNKIRGELRQIVSDPQRMPATKFLISQQTLPDEIKVQI
R*

  Nucleotide sequence: 1926 bp    .

>EFF01818 NZ_CP046844:1067605-1069530 Type VI Lipase Effector (Tle) [Vibrio cholerae C6706]
ATGGATTCATTTAATTATTGCGTGCAGTGTAACCCTGAGGAAAACTGGTTAGAGCTAGAGTTTCGGAGTGAAAATGATGA
ACCGATAGATGGTTTGCTAGTTACGATTACGAACCAAAGTGCCCCCTCTAACACATACACGCAAACCACAAGTTCGGGTA
AGGTGTTGTTTGGCAAGATTGCGGCTGGGGAATGGCGTGCTTCTGTAAGCCAAGCTTCTCTGTTAACTGAAGTCGAAAAA
TATGCCAGCCGTAAAGAGGGACAAGAGTCGCCAGTGAAAAAACGAGCTGCGGCAGAACTTGACGCCGCAGATAAAGATAC
GAAGCAGTACCGCTTTACCACTATTGGTGATTTCTGGGATGAAGCACCAAAAGATGAGTTTTTACAAAAGCAGCATAAAG
GGATAGATGTCAATGCTTCTGCTGAGAAAGCAGGGTTTAGACTCAGTCATAACCAGACCTATGTTTTTGAAATCAAAGCA
CTACGTAGCTATATGCCAGTGATTATTGATACCGATGAATTTAACTTGGTGAATAGTTATACCTTTGCGTTGTTGTCGAA
ATTAGCTTATGCGACTAACGATTTTAATAGGGATGATGGTAAGACAATAGATAATCAGGGAGCAATTAGCACGGTTATTT
CTCAATTGAAGCGTAAAGAAAGACCGACTTATTCTGGAGATTTACAAGCAAAGTGGTTATTAGAGGAAATTCCATATAGC
AAAGCGTTAAGTGCCCAATATTATGCTGAGGATGATGTCGGTAGTGAAGGTTACATCATCTTTAACGATGAATTGGCGAT
TATAGGTGTGAGAGGTACAGAGCCTTATTTTCAGAGTAAGAAACCACCTGTTGATAACACTAAATTTAAAATTATAAAAG
CGGCTTCAGGTATGGCGGCTGTGATTGCGGATAAGATAGAAAGTGCTACCGATTCACCAGGAATGAAAGACCTTATTATA
ACAGACCTTGATGCTGCGCAAATCGCCCCCGAAGAGTTTGGTGGAACATATGTTCATCGGGGGTTTTACCAATATACGAT
GGCTTTGTTATCTTTGATGGAGAAAGATCTAGGGCTACATAAAATAAAAAAATTCTATTGTTGTGGCCATAGCTTAGGGG
GCGCTGGTGCATTACTTATAAGTGCATTGATTAAAGATAGCTATCATCCTCCAGTATTGCGTTTATATACATATGGAATG
CCACGAGTGGGAACTCGCAGTTTTGTAGAGCGCTATCAAAATATTTTGCATTACCGTCATGTTAATAATCACGATTTGGT
GCCACAGATACCAACAGTATGGATGAACACAGATGTATCTGAAGGATTCCATGTACTTGATGTATTTAAAAGCCGTGTTG
ATCTGATGAGAAAAATGCTCACTGATGATGATGATGACAATTATCAACACCATGGTCATTTGTCTCAGTTACTAACTTAT
AATTCAAATAACCAAGTCTTACTGACACCCAAACAAACTCAAGTCACTATGCTAGACCTAGCAAATTTAGCCACTAATGA
TTCTGTTGCTATGGTAGATGGTTTATCAGATGCCTCAATAGTAGAGCATGGAATGGAGCAGTATATACCCAATTTATTTG
AGCAGTTAACAGCACTAAGTGATGAAAGTTTAATGGTGCATTATCAACGAGCGATTTCAGCCTTAGAGCAGGAAATTGCT
ACGTTGCAACAAAGTTATCTTACAGTTAAACAAGCTTGGATTGAATCTATAGGAAATGGCACGCCGACTATGAATATCGG
ACGTTTGATGAGTGAAATGCATTCTATAAATAAATTAATAGAGAACCGTAATAAAATTAGAGGTGAGCTAAGGCAGATAG
TCTCTGATCCTCAGCGTATGCCTGCAACTAAATTTTTAATTTCACAGCAAACCTTACCAGATGAAATCAAGGTGCAAATA
AGATGA