Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00356  |
| Cognate effector | EFF01818 |
| Related T6SS (Type) | T6SS01168 (Type i1) |
| Strain | Vibrio cholerae C6706 |
| Replicon | chromosome |
| Sequence | Protein sequence (248 a.a.); Nucleotide sequence (744 bp) |
| Description | TsiV1 |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | GPY04_RS04835 (TsiV1) |
| Coordinates (Strand) | 1069527..1070270 (+) |
| NCBI ID | 446694188 |
| RefSeq | NZ_CP046844 |
| Uniprot ID | TSIV1_VIBCH, A0A0X1KWL5_VIBCO, A0A0K9V0E4_VIBCL, A0A0H3Q1U3_VIBCL, C3LMB4_VIBCM, A0A023PTD5_VIBCL |
| KEGG ID | vcq:EN18_10315, vch:VC1419, vcm:VCM66_1374 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-11 | MKLLNNLAIKK |
| Transmembrane helix | 12-30 | LISVVVLFIALFFLGYDYY |
| Outside | 31-247 | QTSQPNIWGDEPDESYITISGKKPIDAYLEVWTHFWVTGDECEAYSYDLFGQKAHQGGKISQKITHDFAKDGSNFEFRIPYQTYKNSQNCIVELRDFSIQAHNDFDTVGFAQLRFSPAGREYYNREVDLNSLITADNCNSDIFKSIRKEWAGAIGCHFYVDGKKKTKDEEFNAYTIYYDFSKFNNDTVIHYDILAGENYRSEPLSSAQKTAVVSDDN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9251 | - | - |
Protein sequence: 248 a.a. .
>IMU00356 NZ_CP046844:1069527-1070270 [Vibrio cholerae C6706]
MKLLNNLAIKKLISVVVLFIALFFLGYDYYQTSQPNIWGDEPDESYITISGKKPIDAYLEVWTHFWVTGDECEAYSYDLF
GQKAHQGGKISQKITHDFAKDGSNFEFRIPYQTYKNSQNCIVELRDFSIQAHNDFDTVGFAQLRFSPAGREYYNREVDLN
SLITADNCNSDIFKSIRKEWAGAIGCHFYVDGKKKTKDEEFNAYTIYYDFSKFNNDTVIHYDILAGENYRSEPLSSAQKT
AVVSDDN*
MKLLNNLAIKKLISVVVLFIALFFLGYDYYQTSQPNIWGDEPDESYITISGKKPIDAYLEVWTHFWVTGDECEAYSYDLF
GQKAHQGGKISQKITHDFAKDGSNFEFRIPYQTYKNSQNCIVELRDFSIQAHNDFDTVGFAQLRFSPAGREYYNREVDLN
SLITADNCNSDIFKSIRKEWAGAIGCHFYVDGKKKTKDEEFNAYTIYYDFSKFNNDTVIHYDILAGENYRSEPLSSAQKT
AVVSDDN*
Nucleotide sequence: 744 bp .
>IMU00356 NZ_CP046844:1069527-1070270 [Vibrio cholerae C6706]
ATGAAGTTATTGAATAATCTTGCAATAAAAAAGCTAATCTCAGTTGTTGTACTATTCATTGCATTATTTTTTCTTGGGTA
TGACTATTACCAGACTTCTCAACCTAATATTTGGGGTGATGAACCTGATGAGTCCTATATTACTATATCAGGCAAAAAGC
CTATAGATGCTTATTTAGAAGTGTGGACTCATTTTTGGGTGACAGGAGATGAGTGTGAAGCTTATTCCTACGATTTATTT
GGTCAAAAAGCCCATCAAGGAGGAAAAATAAGCCAGAAGATTACGCATGATTTTGCTAAAGATGGTTCAAATTTTGAATT
TAGAATTCCTTACCAAACGTATAAGAACTCACAAAATTGTATAGTGGAATTAAGAGACTTTTCCATACAAGCTCATAATG
ATTTTGATACCGTAGGTTTTGCTCAGTTGCGATTTTCTCCAGCAGGCAGAGAATATTACAATCGAGAGGTTGATCTAAAC
TCATTGATTACAGCAGATAATTGCAATAGTGATATATTTAAAAGTATAAGAAAAGAGTGGGCAGGAGCCATTGGTTGTCA
TTTTTATGTCGATGGAAAGAAAAAGACTAAAGATGAAGAATTCAACGCTTACACCATCTACTACGATTTTTCGAAATTCA
ATAATGACACCGTGATTCATTACGATATTTTAGCCGGTGAAAACTATCGGTCAGAGCCTTTGTCCTCAGCTCAAAAGACA
GCAGTGGTATCTGATGATAATTAA
ATGAAGTTATTGAATAATCTTGCAATAAAAAAGCTAATCTCAGTTGTTGTACTATTCATTGCATTATTTTTTCTTGGGTA
TGACTATTACCAGACTTCTCAACCTAATATTTGGGGTGATGAACCTGATGAGTCCTATATTACTATATCAGGCAAAAAGC
CTATAGATGCTTATTTAGAAGTGTGGACTCATTTTTGGGTGACAGGAGATGAGTGTGAAGCTTATTCCTACGATTTATTT
GGTCAAAAAGCCCATCAAGGAGGAAAAATAAGCCAGAAGATTACGCATGATTTTGCTAAAGATGGTTCAAATTTTGAATT
TAGAATTCCTTACCAAACGTATAAGAACTCACAAAATTGTATAGTGGAATTAAGAGACTTTTCCATACAAGCTCATAATG
ATTTTGATACCGTAGGTTTTGCTCAGTTGCGATTTTCTCCAGCAGGCAGAGAATATTACAATCGAGAGGTTGATCTAAAC
TCATTGATTACAGCAGATAATTGCAATAGTGATATATTTAAAAGTATAAGAAAAGAGTGGGCAGGAGCCATTGGTTGTCA
TTTTTATGTCGATGGAAAGAAAAAGACTAAAGATGAAGAATTCAACGCTTACACCATCTACTACGATTTTTCGAAATTCA
ATAATGACACCGTGATTCATTACGATATTTTAGCCGGTGAAAACTATCGGTCAGAGCCTTTGTCCTCAGCTCAAAAGACA
GCAGTGGTATCTGATGATAATTAA