Detailed information of regulator


Summary

Regulator ID REG0033 experimental
Regulator type protein
Regulatory pathway (from literatures) ChiS-induced regulatory pathway
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS01168 experimental (Type i1)
Strain Vibrio cholerae C6706
Replicon chromosome
Sequence Protein sequence (1130 a.a.); Nucleotide sequence (3390 bp)
Description ChiS-induced expression of T6SS genes was initiated by GlcNAc2 when released by chitinases from chitin,and expression of the competence regulators tfoX and tfoR in V.cholerae requires activation of ChiS.
Reference
External database links

Locus tag (Gene) GPY04_RS01040 (ChiS)
Coordinates (Strand) 210606..213995 (-)
NCBI ID 446410424
RefSeq NZ_CP046844
Uniprot ID A0A0K9UGJ8_VIBCL, A0A0H3Q4M4_VIBCL, A0A0H7F5K6_VIBCL
KEGG ID vcq:EN18_06055
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Necessory for TfoX/TfoR ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Response_reg PF00072.26 3.2e-32 725..835
HATPase_c PF02518.28 1.8e-27 574..683
HisKA PF00512.27 1.6e-14 460..525
HAMP PF00672.27 1.9e-12 375..427
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-14 MFRFYRKQKFKRLQ
Transmembrane helix 15-37 NTLMAAFLALSIIPLTITALFFL
Inside 38-355 HSHSKDLEQQSTSYLVSVRDNKQQQVIDYMMAKESEVMGFVRSELAYASGGRFYGLVNAFQRLDVSIEAAREHAQQRYITGSGDQIKTSVLPQSSSYVGSERYRLLHKRYHWAYLELLKRSDFDDILLVDIDGNVVYSIYKYDNFGTNLLTGKYQDTNLGHTFKRLEQTVNEQRKTNEDFTPVVISDFVQEDGKQYAWLGAPIIQQGYLHSYAMFRLPSNAITKLIAEGSSNPSMQTILVGQDHRSRTLTSAEIAVEKSKAVVDLALSGKRAVGTYTNTDGEQIIAAYAPINLKNIHWALVVELPEKEAFARVRQLEK
Transmembrane helix 356-375 LFVFAMLTAIVLVVIASHYL
Outside 376-1129 SNFITSPLLKLTWAAERVSAGDLDEAMINTERKDEIGRLAVSFERMQRSIREKISLIKSQNKELESNLLIIRKQNDELQLANKLKDEFLATTSHELRTPLHGMIGIAEALISGANGPISAAHKYQLDIIISSGQRLATLVDDLLDYHKMRYGALDIQRCAVDLSSATRLVLELSHHLLGKKTLRIINQVSEQPVWVSADPQRLEQVLYNLIGNAIKYTSEGKIVISATYIDDKVRVQVVDTGQGIPAEQLEHIFEPLIQAGHDASRYRQGAGLGLSISRQLIELMHGTLYVSSQPMVGTTFSFTLPLASEEEIAQARLTELPHFQAPEVLDSELPEQSNLPENEHGPLLLVADDEPVNLRVLDSFLRLEGYRVHTAQDGHQVLEAIKREKPELVLLDIMMPGMSGYQVCEKLRQTYDHAELPIIMLTALNQSDDRVRGFEAGANDYLSKPFNKQELAARIVAHLTASKAELRRIENAQLQKELKHRAMVEASLLETQGRLLEQLESAPEAILCVKEGNKVRFANEAAARLFRRTPEQLKRSNAEELIAPKFLNIEQEHYCGNIDVYVDDVRQHLSADVLRLPQGSGLQAMYIFNVGGSVNAARIHNLETAVEALSSYAFEGDKDKLQKLKELGGEFTRLADKASGEYQSKQDLMRAVLVEAMTSALNYWERVSGQSKFTFAEQSGLWRVYLDRSTLQTRTLDKYLRIETLPKTPRWRTVLNSLDYILEHCKEAGPERTHIEMQRDKLQKLLTSE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9604 - -


  Protein sequence of regulator: 1130 a.a.    .

>REG0033 NZ_CP046844:c213995-210606 [Vibrio cholerae C6706]
MFRFYRKQKFKRLQNTLMAAFLALSIIPLTITALFFLHSHSKDLEQQSTSYLVSVRDNKQQQVIDYMMAKESEVMGFVRS
ELAYASGGRFYGLVNAFQRLDVSIEAAREHAQQRYITGSGDQIKTSVLPQSSSYVGSERYRLLHKRYHWAYLELLKRSDF
DDILLVDIDGNVVYSIYKYDNFGTNLLTGKYQDTNLGHTFKRLEQTVNEQRKTNEDFTPVVISDFVQEDGKQYAWLGAPI
IQQGYLHSYAMFRLPSNAITKLIAEGSSNPSMQTILVGQDHRSRTLTSAEIAVEKSKAVVDLALSGKRAVGTYTNTDGEQ
IIAAYAPINLKNIHWALVVELPEKEAFARVRQLEKLFVFAMLTAIVLVVIASHYLSNFITSPLLKLTWAAERVSAGDLDE
AMINTERKDEIGRLAVSFERMQRSIREKISLIKSQNKELESNLLIIRKQNDELQLANKLKDEFLATTSHELRTPLHGMIG
IAEALISGANGPISAAHKYQLDIIISSGQRLATLVDDLLDYHKMRYGALDIQRCAVDLSSATRLVLELSHHLLGKKTLRI
INQVSEQPVWVSADPQRLEQVLYNLIGNAIKYTSEGKIVISATYIDDKVRVQVVDTGQGIPAEQLEHIFEPLIQAGHDAS
RYRQGAGLGLSISRQLIELMHGTLYVSSQPMVGTTFSFTLPLASEEEIAQARLTELPHFQAPEVLDSELPEQSNLPENEH
GPLLLVADDEPVNLRVLDSFLRLEGYRVHTAQDGHQVLEAIKREKPELVLLDIMMPGMSGYQVCEKLRQTYDHAELPIIM
LTALNQSDDRVRGFEAGANDYLSKPFNKQELAARIVAHLTASKAELRRIENAQLQKELKHRAMVEASLLETQGRLLEQLE
SAPEAILCVKEGNKVRFANEAAARLFRRTPEQLKRSNAEELIAPKFLNIEQEHYCGNIDVYVDDVRQHLSADVLRLPQGS
GLQAMYIFNVGGSVNAARIHNLETAVEALSSYAFEGDKDKLQKLKELGGEFTRLADKASGEYQSKQDLMRAVLVEAMTSA
LNYWERVSGQSKFTFAEQSGLWRVYLDRSTLQTRTLDKYLRIETLPKTPRWRTVLNSLDYILEHCKEAGPERTHIEMQRD
KLQKLLTSE*

  Nucleotide sequence of regulator: 3390 bp    .

>REG0033 NZ_CP046844:c213995-210606 [Vibrio cholerae C6706]
ATGTTTAGGTTCTATCGAAAACAAAAATTCAAACGCCTGCAAAATACCTTGATGGCTGCGTTTTTGGCCCTCAGCATTAT
TCCTTTGACCATTACTGCGTTGTTTTTCCTACATTCACACAGTAAAGATCTGGAGCAGCAGAGCACTTCTTACCTCGTTT
CTGTTCGGGATAATAAACAGCAACAGGTCATCGACTACATGATGGCCAAAGAGTCGGAAGTGATGGGTTTTGTTCGCTCC
GAGCTGGCTTATGCCAGTGGCGGGCGTTTTTATGGTCTGGTGAATGCTTTCCAGCGTTTAGACGTCAGTATTGAAGCGGC
TCGTGAACATGCTCAGCAGCGCTATATCACCGGATCGGGTGACCAAATAAAAACCTCGGTGCTCCCCCAATCAAGCAGCT
ACGTGGGCAGTGAACGCTATCGACTGCTGCATAAACGCTACCACTGGGCTTACTTAGAACTGCTGAAACGGTCGGACTTT
GATGACATTTTATTGGTCGATATTGATGGCAACGTCGTTTATTCGATTTATAAATACGACAATTTTGGCACCAACTTATT
AACGGGCAAATACCAAGACACCAATCTAGGCCATACCTTCAAGCGTTTAGAACAAACGGTCAATGAACAGCGCAAAACCA
ACGAAGATTTCACCCCTGTCGTGATTTCTGATTTCGTACAAGAGGATGGTAAACAATACGCTTGGCTTGGCGCACCGATT
ATTCAGCAAGGCTACTTGCATAGTTACGCGATGTTCCGCTTACCAAGCAATGCGATCACCAAACTCATCGCAGAAGGCAG
TAGCAATCCTTCGATGCAAACCATTCTAGTGGGACAAGATCACCGCTCAAGAACCTTAACTTCCGCCGAAATTGCGGTTG
AAAAAAGTAAAGCTGTGGTCGATTTAGCCTTATCGGGCAAACGCGCCGTAGGCACTTACACCAATACCGATGGTGAACAG
ATCATCGCGGCCTACGCACCGATCAATTTGAAAAATATCCATTGGGCTCTGGTGGTTGAACTGCCAGAGAAAGAAGCGTT
TGCTCGCGTTCGTCAGTTAGAGAAACTGTTTGTTTTTGCGATGTTGACGGCTATCGTCTTAGTGGTCATCGCCTCGCATT
ACCTTTCCAATTTCATTACCTCACCGCTTTTGAAGCTGACATGGGCAGCCGAACGTGTTTCGGCAGGCGATCTTGATGAA
GCCATGATCAATACCGAACGCAAAGATGAAATTGGCCGCTTGGCGGTCAGTTTTGAGCGTATGCAGCGTTCCATTCGCGA
AAAAATATCGCTGATCAAATCGCAAAATAAAGAGTTAGAGAGCAACTTACTGATCATTCGTAAGCAAAACGATGAGTTAC
AGCTAGCGAATAAGCTAAAAGATGAGTTCCTCGCGACCACATCACATGAATTACGCACCCCATTGCATGGCATGATTGGT
ATTGCCGAAGCCCTGATCTCGGGGGCAAATGGCCCGATTTCAGCAGCGCACAAGTATCAACTCGACATCATTATCAGCAG
TGGGCAGCGCTTGGCGACTCTGGTTGATGATCTACTCGATTACCACAAAATGCGCTATGGCGCGCTGGATATTCAAAGGT
GTGCCGTCGATTTATCGAGCGCTACGCGCTTAGTCTTGGAGCTTTCTCACCATTTACTTGGCAAGAAAACGCTGCGCATT
ATTAATCAAGTCTCAGAACAACCGGTCTGGGTTTCTGCGGATCCTCAACGCTTAGAGCAAGTCCTGTATAACTTGATCGG
CAATGCCATTAAGTACACTTCCGAAGGCAAAATTGTTATCTCCGCAACCTATATTGACGATAAAGTGCGCGTGCAGGTGG
TGGATACAGGTCAAGGGATTCCGGCAGAACAACTGGAGCACATCTTTGAGCCATTGATTCAAGCGGGCCATGATGCGAGT
CGCTATCGCCAAGGCGCAGGACTCGGGCTTTCCATCAGTCGTCAGTTAATCGAATTGATGCACGGCACTCTCTACGTAAG
TAGTCAGCCGATGGTTGGCACTACCTTTAGCTTCACGCTGCCTCTGGCGAGCGAAGAAGAGATTGCTCAAGCGCGCTTGA
CCGAACTCCCCCACTTCCAAGCACCGGAAGTGTTGGATTCTGAACTGCCCGAGCAAAGCAACTTACCCGAAAATGAACAT
GGCCCACTTCTGTTGGTCGCCGATGATGAACCAGTCAACCTGCGTGTGCTGGACAGCTTTTTACGTCTTGAAGGTTATCG
CGTACATACCGCGCAAGATGGCCATCAAGTACTTGAAGCAATTAAACGTGAAAAACCTGAATTGGTGTTACTCGACATCA
TGATGCCAGGCATGAGCGGTTATCAAGTGTGTGAAAAACTTCGACAAACTTACGACCACGCTGAACTGCCTATTATTATG
CTGACGGCTCTCAACCAGTCTGATGACCGGGTACGAGGGTTTGAAGCTGGAGCCAATGACTACTTATCCAAACCATTCAA
CAAACAAGAGCTGGCCGCGCGCATTGTCGCTCACCTCACGGCGAGCAAAGCCGAACTGCGCCGCATTGAGAATGCCCAAC
TGCAAAAAGAGTTGAAACATCGCGCTATGGTAGAAGCGAGCCTATTGGAAACCCAAGGCCGATTATTGGAACAGTTAGAA
TCTGCGCCAGAAGCGATTTTGTGCGTTAAAGAGGGCAATAAAGTACGTTTTGCTAACGAAGCGGCAGCACGTTTATTTAG
ACGTACCCCTGAGCAGCTCAAACGCTCCAACGCCGAAGAACTGATTGCTCCTAAATTCTTGAATATTGAGCAAGAGCATT
ACTGCGGCAATATTGATGTTTACGTAGATGATGTGCGCCAGCACCTCTCTGCCGATGTCCTTCGCTTGCCGCAAGGTTCT
GGCTTACAAGCGATGTACATTTTTAATGTCGGCGGCAGCGTCAATGCCGCGCGGATTCATAACCTCGAAACCGCGGTAGA
AGCCCTGTCTAGCTATGCCTTCGAAGGCGATAAAGACAAGCTGCAAAAACTCAAGGAACTGGGTGGTGAGTTTACTCGTC
TTGCAGATAAAGCCTCGGGCGAGTATCAATCGAAACAAGATTTGATGCGTGCCGTGTTAGTCGAAGCCATGACCAGTGCC
TTGAACTATTGGGAACGAGTCTCAGGGCAAAGCAAGTTCACGTTTGCCGAACAAAGTGGCTTGTGGCGCGTTTATCTTGA
CCGCAGCACCCTACAAACTCGTACCCTAGACAAATACTTACGAATTGAGACACTGCCTAAAACACCGCGTTGGCGAACCG
TACTGAACTCGCTCGACTACATTCTTGAGCATTGCAAAGAAGCAGGCCCTGAACGCACTCACATCGAAATGCAGCGCGAT
AAATTGCAAAAACTCCTGACCAGTGAATAA