SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01820
Effector type	Tme
Cognate immunity protein	IMU00357
Related T6SS (Type)	T6SS01168 (Type i1)
Strain	Vibrio cholerae C6706
Replicon	chromosome
Sequence	Protein sequence (1086 a.a.); Nucleotide sequence (3258 bp)
Description	VasX protein
Reference	[1] Logan SL, Thomas J, Yan J, et al. The Vibrio cholerae type VI secretion system can modulate host intestinal mechanics to displace gut bacterial symbionts. Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):E3779-E3787. doi: 10.1073/pnas.1720133115. Epub 2018 Apr 2. PMID: 29610339

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	GPY04_RS16385 (VasX)
Coordinates (Strand)	405867..409124 (-)
NCBI ID	445992499
RefSeq	NZ_CP046845
Uniprot ID	UPI0001BAE095
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-756	MSNPNQAAKTGQTNDAQNPASACPFKQPLIGIIPVRYAFDVYDDQGQALHPLPKADRQWKGQFSIKQRSYTLRQLRDGWLYVYDETAKTLHEYEVVGCKLTKIDWSDDEANKPTHERGSKGESKSCLLYPAQHTLSIGYAHQRWTWRVCEHMRSNTSSRHAVMRKVSLKQFESNGTHPHAHFAQYLEDYVADIGTPAEQDIFKDTCTPSLPVEKSEEAVKGTEFKFVADKAVVSSSDYLQDLPEQNCGLFVALNDPLADVSDLFVTFTTQVAKRTKAIGDETQQHKMQMAELTRTLGRIRLEEKEIPDFVKQDPIRILELERAITEYCATAKLAEIESHHLASEGHSPSGNYALMQQQAEQKLAELKTLYRFEPTSAQMRKWRKKDNSFIDEVRWADLDNFLVEHYTELKGLDEQIKQHYAQFMSAFNQLGLDPLLFGMDNQDEVQQAYLLALTSQFLVVVTQVNHDEKSLEILKKDLSFDSPKNLMALASTGFSLQANQAINNHIQGFSTAFLSTSNPSDMVAFATAIANWDTFTGDERIQEKAWFKRWIEPVQSSFGALQKAVANQAKESWQAVMELLFPYQNQPKGGTPSLLANLRLLLVESLVREEAVLQHNPKYAAELKQFETKLNAILQEMNDALELKPGNVSPKNHQIATAQSAQRKLGQLLSSELPMMLTLKNQAAMNTFQQSVNEKLSALSKNVKTSSASVSQKLGGLGGLLFALNLWNTMTVLENIRYKVAQYPSWNPFKNPALGE
Transmembrane helix	757-779	AIYATGNTIVVAGAISAGRAWVT
Inside	780-859	IAEQGLLDRTLKNALNTTKVLGTKDALKTFAKSIALVATVGMIASALETWESWGKFNDSSKTDLERFGYLLKAGATGAQG
Transmembrane helix	860-882	IIFYIQFFTLLGSGIGGPSIAAI
Outside	883-885	SAG
Transmembrane helix	886-908	WMLAGFAVIGIVYLIGVILTNVF
Inside	909-1085	KRSELEIWLSKSTWGKESAHWPVGKELTELEHLLHRPSLRLSQVTQRKAAQWMDSGSLQWQLELTLPDYLKGQTIGLQITRLPAQPAYYQPQREAVTPILINEQQGKWSIEDNQPVYRITLGGSEKDTVGVCVALPLRWGKELSLKFYASGTRAGELDLQSAEANDIATRNLVVGKG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9607	-	-

Protein sequence: 1086 a.a. .

                                    >EFF01820 NZ_CP046845:c409124-405867 Type VI Membrane-disrupting Effector (Tme) [Vibrio cholerae C6706]
MSNPNQAAKTGQTNDAQNPASACPFKQPLIGIIPVRYAFDVYDDQGQALHPLPKADRQWKGQFSIKQRSYTLRQLRDGWL
YVYDETAKTLHEYEVVGCKLTKIDWSDDEANKPTHERGSKGESKSCLLYPAQHTLSIGYAHQRWTWRVCEHMRSNTSSRH
AVMRKVSLKQFESNGTHPHAHFAQYLEDYVADIGTPAEQDIFKDTCTPSLPVEKSEEAVKGTEFKFVADKAVVSSSDYLQ
DLPEQNCGLFVALNDPLADVSDLFVTFTTQVAKRTKAIGDETQQHKMQMAELTRTLGRIRLEEKEIPDFVKQDPIRILEL
ERAITEYCATAKLAEIESHHLASEGHSPSGNYALMQQQAEQKLAELKTLYRFEPTSAQMRKWRKKDNSFIDEVRWADLDN
FLVEHYTELKGLDEQIKQHYAQFMSAFNQLGLDPLLFGMDNQDEVQQAYLLALTSQFLVVVTQVNHDEKSLEILKKDLSF
DSPKNLMALASTGFSLQANQAINNHIQGFSTAFLSTSNPSDMVAFATAIANWDTFTGDERIQEKAWFKRWIEPVQSSFGA
LQKAVANQAKESWQAVMELLFPYQNQPKGGTPSLLANLRLLLVESLVREEAVLQHNPKYAAELKQFETKLNAILQEMNDA
LELKPGNVSPKNHQIATAQSAQRKLGQLLSSELPMMLTLKNQAAMNTFQQSVNEKLSALSKNVKTSSASVSQKLGGLGGL
LFALNLWNTMTVLENIRYKVAQYPSWNPFKNPALGEAIYATGNTIVVAGAISAGRAWVTIAEQGLLDRTLKNALNTTKVL
GTKDALKTFAKSIALVATVGMIASALETWESWGKFNDSSKTDLERFGYLLKAGATGAQGIIFYIQFFTLLGSGIGGPSIA
AISAGWMLAGFAVIGIVYLIGVILTNVFKRSELEIWLSKSTWGKESAHWPVGKELTELEHLLHRPSLRLSQVTQRKAAQW
MDSGSLQWQLELTLPDYLKGQTIGLQITRLPAQPAYYQPQREAVTPILINEQQGKWSIEDNQPVYRITLGGSEKDTVGVC
VALPLRWGKELSLKFYASGTRAGELDLQSAEANDIATRNLVVGKG*

                                

Nucleotide sequence: 3258 bp .

                                    >EFF01820 NZ_CP046845:c409124-405867 Type VI Membrane-disrupting Effector (Tme) [Vibrio cholerae C6706]
ATGAGTAATCCCAATCAAGCTGCGAAAACAGGACAGACCAATGATGCGCAAAATCCCGCCAGTGCATGTCCTTTTAAACA
GCCGTTAATAGGGATAATTCCTGTTCGTTACGCCTTTGATGTTTATGATGATCAAGGTCAAGCATTACATCCTTTACCCA
AAGCGGATCGACAGTGGAAAGGTCAATTCTCTATCAAGCAGCGCAGTTACACTTTAAGGCAACTGCGTGATGGCTGGCTC
TATGTCTATGATGAAACAGCAAAAACACTGCACGAATATGAAGTCGTTGGCTGCAAGTTGACCAAAATTGATTGGTCTGA
TGATGAGGCAAACAAACCCACTCACGAGCGCGGTTCGAAAGGTGAAAGCAAAAGCTGTTTACTCTATCCCGCCCAGCACA
CGCTCTCTATCGGTTATGCGCATCAACGATGGACGTGGCGAGTGTGTGAGCATATGCGCTCCAATACCAGCAGCCGTCAT
GCTGTGATGCGTAAAGTGAGCCTTAAACAGTTTGAGTCCAATGGTACTCACCCTCATGCACATTTTGCTCAGTATCTTGA
AGACTATGTGGCGGACATCGGGACGCCAGCTGAGCAGGATATTTTTAAAGATACCTGTACCCCTTCATTACCGGTAGAGA
AAAGTGAAGAAGCGGTGAAAGGTACGGAATTCAAATTCGTTGCCGACAAAGCGGTGGTAAGCAGCAGTGATTATTTGCAA
GATTTGCCGGAGCAAAACTGCGGTTTATTTGTGGCGCTGAATGACCCTTTAGCGGATGTGTCAGATTTATTTGTTACTTT
TACTACCCAAGTGGCAAAGCGAACTAAAGCGATTGGTGATGAAACGCAACAGCATAAAATGCAGATGGCAGAGCTGACAC
GCACATTAGGCCGAATTCGTCTTGAAGAAAAAGAAATTCCCGATTTTGTAAAACAAGATCCGATCCGTATTTTAGAGCTA
GAAAGAGCCATTACTGAATATTGTGCTACGGCAAAATTGGCCGAAATCGAATCTCATCATTTAGCCAGTGAAGGGCATTC
CCCATCGGGAAACTATGCCTTGATGCAGCAGCAAGCGGAGCAGAAACTGGCGGAATTGAAAACACTCTATCGCTTTGAAC
CCACCAGTGCTCAGATGCGCAAGTGGCGCAAAAAAGACAACAGCTTTATTGATGAAGTGCGTTGGGCGGATTTAGATAAC
TTTTTGGTGGAGCATTACACCGAGCTCAAAGGATTGGATGAACAGATTAAACAACACTATGCGCAGTTTATGTCTGCCTT
CAACCAGCTCGGCCTTGACCCGCTTCTGTTTGGTATGGATAACCAAGATGAGGTTCAGCAAGCGTATTTGTTAGCATTAA
CTAGCCAATTTTTAGTGGTGGTTACGCAAGTCAATCATGATGAAAAATCGCTTGAGATCCTAAAAAAAGATCTCAGTTTT
GATTCACCGAAAAACCTCATGGCCCTCGCGTCAACAGGCTTTTCCCTGCAGGCAAATCAAGCCATTAACAACCATATCCA
AGGGTTTAGCACCGCTTTTCTCTCTACAAGTAATCCTAGTGATATGGTGGCGTTTGCGACCGCAATCGCTAACTGGGATA
CGTTCACGGGGGATGAACGTATTCAGGAGAAAGCATGGTTTAAGAGATGGATTGAACCCGTTCAATCGAGTTTTGGTGCA
CTGCAAAAAGCGGTCGCCAATCAAGCCAAAGAGAGTTGGCAAGCGGTGATGGAGTTGCTGTTTCCTTATCAGAATCAGCC
CAAAGGAGGGACGCCCAGTTTGTTGGCTAACCTTCGTTTACTGTTGGTGGAAAGCCTAGTTCGAGAAGAGGCGGTGCTGC
AACATAACCCCAAGTATGCGGCTGAGCTTAAGCAATTTGAGACTAAGCTGAATGCCATTTTGCAAGAGATGAATGATGCT
CTGGAACTCAAACCCGGTAATGTGAGCCCGAAAAACCATCAAATTGCGACCGCGCAATCCGCACAACGTAAGCTAGGGCA
ATTACTCAGTAGTGAGCTGCCGATGATGCTGACGCTCAAAAACCAAGCTGCGATGAACACTTTTCAGCAGTCGGTAAATG
AGAAACTGAGCGCGCTATCGAAAAACGTCAAAACCAGTAGCGCGTCGGTGAGTCAGAAACTGGGTGGATTAGGGGGCTTG
CTGTTTGCGTTGAACCTATGGAATACCATGACGGTTTTGGAGAATATCCGCTATAAGGTCGCACAATACCCCAGTTGGAA
TCCTTTTAAGAACCCGGCGTTGGGCGAAGCCATTTATGCCACTGGCAACACTATTGTAGTCGCCGGTGCCATTAGTGCTG
GGCGAGCTTGGGTGACGATTGCTGAGCAAGGGTTGTTAGATCGTACACTGAAAAACGCCTTAAATACCACGAAAGTTTTG
GGCACAAAAGATGCCCTAAAAACCTTTGCTAAATCGATCGCCTTGGTGGCTACCGTGGGCATGATTGCCAGCGCATTAGA
AACATGGGAAAGCTGGGGTAAGTTTAATGACAGCAGTAAAACCGATCTTGAACGCTTCGGTTATTTGTTAAAAGCTGGAG
CAACGGGAGCACAGGGAATTATTTTTTATATTCAGTTTTTCACACTCTTAGGTAGTGGAATTGGTGGACCCTCCATAGCT
GCGATTTCTGCAGGTTGGATGCTCGCCGGTTTTGCTGTGATTGGTATTGTCTATTTGATCGGGGTGATTTTGACCAATGT
GTTTAAACGCTCAGAGTTAGAAATTTGGTTATCAAAATCGACGTGGGGGAAAGAGAGTGCTCATTGGCCAGTGGGCAAAG
AATTGACCGAGTTAGAGCACCTTCTCCATCGTCCGAGTCTTCGGTTGAGTCAAGTTACGCAACGTAAAGCAGCGCAATGG
ATGGATTCAGGTTCTTTACAATGGCAGTTGGAACTCACGTTACCCGATTATCTTAAGGGGCAGACGATAGGCTTACAAAT
CACCCGTTTGCCAGCGCAGCCGGCATATTATCAACCGCAAAGGGAGGCGGTAACACCAATACTCATCAATGAACAACAGG
GCAAATGGAGTATTGAAGATAATCAGCCAGTGTATCGTATTACACTGGGTGGCAGTGAGAAGGATACTGTCGGAGTTTGC
GTTGCACTGCCCCTGCGTTGGGGCAAGGAGCTGAGCCTCAAATTCTATGCCAGTGGAACTCGCGCGGGGGAATTGGATTT
ACAATCTGCAGAGGCGAATGATATTGCCACAAGAAATCTCGTTGTAGGAAAAGGTTAA

                                