Detailed information of regulator


Summary

Regulator ID REG0035 experimental
Regulator type protein
Regulatory pathway (from literatures) ChiS-induced regulatory pathway
KEGG pathway (determined by BlastKOALA) Metabolic pathways (map01100); Amino sugar and nucleotide sugar metabolism (map00520)
Related T6SS T6SS01168 experimental (Type i1)
Strain Vibrio cholerae C6706
Replicon chromosome
Sequence Protein sequence (847 a.a.); Nucleotide sequence (2541 bp)
Description ChiS-induced expression of T6SS genes was initiated by GlcNAc2 when released by chitinases(chiA1) from chitin.
Reference
External database links

Locus tag (Gene) GPY04_RS07700 (chiA1)
Coordinates (Strand) 1709613..1712153 (-)
NCBI ID 446755770
RefSeq NZ_CP046844
Uniprot ID Q9KQP6_VIBCH, A0A0X1KXS0_VIBCO, A0A0H3Q380_VIBCL, C3LNQ6_VIBCM, A0A0H5WNU7_VIBCL
KEGG ID vcq:EN18_12870, vch:VC1952, vcm:VCM66_1876
KEGG pathway (determined by BlastKOALA) map01100; map00520
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
GPY04_RS01040 (ChiS) ↓ 446410424
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Glyco_hydro_18 PF00704.30 9.2e-26 523..824
Big_7 PF17957.3 1.2e-15 179..246
Big_7 PF17957.3 9.4e-15 336..404
Big_7 PF17957.3 1.3e-12 85..150
Big_7 PF17957.3 3e-12 428..494
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-846 MKRYCLAAVITASLGVSYSAQAYNCAGVPVWDSSTVYVGSDKVQKTNTAYQARYWTQGNDPVTHSGQWDAWQILGQCDGGANNPPQVSIQSPLNNAKIPQGSVVGLQANASDSDGSITQVEFLVGTQRIAIDQQAPYQVDWTATLGATSVTAIATDNQGATTSSTVNISVTPTGNPVPPTVTLTSPTGSEQLTVGDVLAVAANATDSDGTVNAVEFYVDGQLVVIDSSEPYQFNWNAAVGSHTFKAKAIDNDNLSTLSQEVTLTVGSGSNAGCAGLPVYSVGTAYSAGQLVQNKNQKYRCDIAGWCSSSSGWAYEPGVGSYWKEAWSGLGACSTPPVVTLTNPTANQVILAGSTVSVAAQASDADGSVMQVEFFAGNNSLGVVTQAPYAVNWLATTTGNQTLKAVATDNDSNTSESAVSVTVSDQDLVVSLTSPTSGQTVGLGKPVNIAADATSLTNNVAKVEFVVNGAVVATDTTEPFAYSWTPSAIGNYTVAAKATDAAGTSVTSSAAAISVVEQAQKKHRLIGYWHNFVNGAGCPIRLADMSQAWDVIDIAFAENDRNSTGTVHFNLYAGDIYSSCPALDPAQFKQDMKALQAKGKVFVLSLGGAEGTITLNTDQDEANFVSSLTALIKEWGFDGLDVDLESGSNLVHGSQIQARLGRALKQIEKNIGGDMFLTMAPEHPYVQGGMVAYSGIWGAYIPVINEVRDTLDILHVQLYNNGGLPNPYTPSAAPEGSVDMMVAQSKMLIEGFTLANGTRFEPLRDDQVAIGLPSGPSSANSGQAPTQNILDALDCLTKGTRCGTIKPAFAYPNYAGVMTWSINWDKHDGFNFSKPVGDKLSQMNNAQ


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9988 pos: 22-23. AQA-YN. MKRYCLAAVITASLGVSYSAQA


  Protein sequence of regulator: 847 a.a.    .

>REG0035 NZ_CP046844:c1712153-1709613 [Vibrio cholerae C6706]
MKRYCLAAVITASLGVSYSAQAYNCAGVPVWDSSTVYVGSDKVQKTNTAYQARYWTQGNDPVTHSGQWDAWQILGQCDGG
ANNPPQVSIQSPLNNAKIPQGSVVGLQANASDSDGSITQVEFLVGTQRIAIDQQAPYQVDWTATLGATSVTAIATDNQGA
TTSSTVNISVTPTGNPVPPTVTLTSPTGSEQLTVGDVLAVAANATDSDGTVNAVEFYVDGQLVVIDSSEPYQFNWNAAVG
SHTFKAKAIDNDNLSTLSQEVTLTVGSGSNAGCAGLPVYSVGTAYSAGQLVQNKNQKYRCDIAGWCSSSSGWAYEPGVGS
YWKEAWSGLGACSTPPVVTLTNPTANQVILAGSTVSVAAQASDADGSVMQVEFFAGNNSLGVVTQAPYAVNWLATTTGNQ
TLKAVATDNDSNTSESAVSVTVSDQDLVVSLTSPTSGQTVGLGKPVNIAADATSLTNNVAKVEFVVNGAVVATDTTEPFA
YSWTPSAIGNYTVAAKATDAAGTSVTSSAAAISVVEQAQKKHRLIGYWHNFVNGAGCPIRLADMSQAWDVIDIAFAENDR
NSTGTVHFNLYAGDIYSSCPALDPAQFKQDMKALQAKGKVFVLSLGGAEGTITLNTDQDEANFVSSLTALIKEWGFDGLD
VDLESGSNLVHGSQIQARLGRALKQIEKNIGGDMFLTMAPEHPYVQGGMVAYSGIWGAYIPVINEVRDTLDILHVQLYNN
GGLPNPYTPSAAPEGSVDMMVAQSKMLIEGFTLANGTRFEPLRDDQVAIGLPSGPSSANSGQAPTQNILDALDCLTKGTR
CGTIKPAFAYPNYAGVMTWSINWDKHDGFNFSKPVGDKLSQMNNAQ*

  Nucleotide sequence of regulator: 2541 bp    .

>REG0035 NZ_CP046844:c1712153-1709613 [Vibrio cholerae C6706]
ATGAAGCGCTATTGTTTAGCCGCCGTGATTACCGCCAGTTTGGGGGTAAGTTACTCAGCACAAGCCTATAACTGTGCCGG
AGTGCCAGTGTGGGACAGCAGTACAGTGTATGTTGGCAGTGATAAAGTTCAAAAAACCAACACCGCTTACCAAGCGCGTT
ACTGGACTCAGGGTAACGATCCGGTAACCCATTCTGGTCAATGGGACGCATGGCAAATTCTTGGACAGTGTGACGGAGGG
GCGAATAACCCACCTCAAGTGTCCATTCAATCACCACTCAATAACGCCAAGATCCCACAAGGCTCCGTCGTCGGATTACA
AGCCAATGCCTCAGATAGTGATGGCAGCATTACTCAGGTGGAGTTTTTAGTCGGCACACAGCGTATCGCGATTGACCAAC
AAGCCCCATACCAAGTGGATTGGACGGCAACGCTAGGAGCCACTTCAGTGACCGCGATTGCGACCGATAACCAAGGTGCA
ACCACCAGCAGCACCGTAAATATCAGCGTAACGCCTACTGGTAACCCAGTTCCGCCAACTGTGACGTTGACTAGCCCAAC
GGGCAGTGAACAGCTAACCGTGGGCGATGTTCTAGCCGTGGCCGCAAATGCCACTGACAGTGATGGCACTGTCAACGCTG
TTGAATTTTATGTTGATGGCCAACTTGTTGTGATCGATAGCTCTGAGCCTTATCAGTTTAATTGGAATGCCGCTGTCGGC
AGCCATACCTTTAAAGCCAAAGCGATTGATAACGACAACCTGTCTACCCTGAGCCAAGAAGTGACTTTAACGGTCGGCAG
TGGCTCGAATGCAGGTTGCGCGGGTTTACCTGTGTATTCGGTGGGAACCGCGTATTCGGCAGGGCAGTTGGTACAAAATA
AGAACCAAAAATATCGCTGTGATATCGCCGGTTGGTGTTCTTCCAGCTCCGGTTGGGCGTATGAGCCAGGGGTTGGAAGT
TATTGGAAAGAAGCATGGAGTGGTTTAGGCGCTTGTTCAACGCCACCTGTCGTGACATTAACCAACCCAACGGCGAACCA
AGTGATTCTTGCTGGCTCAACCGTCAGTGTGGCGGCGCAAGCCAGTGATGCCGATGGCTCGGTCATGCAAGTGGAGTTTT
TTGCGGGCAACAACAGCTTAGGCGTGGTGACTCAAGCACCTTACGCGGTTAACTGGCTAGCCACTACTACGGGAAATCAA
ACCCTGAAAGCAGTCGCAACCGACAACGACAGTAACACCAGTGAAAGCGCAGTCAGCGTGACGGTGAGCGATCAAGATCT
GGTGGTTTCACTGACTTCACCGACATCCGGCCAAACCGTTGGTCTAGGTAAACCGGTCAATATTGCCGCCGATGCTACGT
CACTAACCAATAATGTGGCGAAAGTGGAGTTTGTGGTCAACGGCGCGGTAGTTGCAACGGATACAACAGAGCCTTTTGCT
TACAGTTGGACGCCAAGTGCGATTGGTAACTATACCGTTGCCGCGAAAGCGACTGACGCAGCAGGAACGAGCGTGACCTC
TTCTGCTGCCGCAATCAGCGTGGTAGAGCAAGCGCAGAAGAAACATCGCTTGATTGGTTACTGGCATAACTTCGTCAATG
GTGCGGGTTGCCCAATTCGTTTGGCGGATATGTCACAAGCGTGGGATGTGATTGATATTGCCTTTGCAGAAAACGATCGC
AACAGTACCGGAACGGTACATTTCAACCTGTATGCCGGTGATATTTACAGCAGTTGTCCTGCGCTCGATCCTGCGCAATT
CAAGCAAGATATGAAAGCACTGCAAGCGAAAGGTAAAGTATTTGTCCTTTCCTTAGGTGGCGCTGAAGGCACTATTACCC
TTAACACTGATCAAGATGAAGCCAACTTCGTGAGCAGCCTGACTGCGTTGATCAAAGAATGGGGCTTTGATGGGTTGGAT
GTGGATCTCGAAAGTGGTTCAAACCTTGTTCATGGCTCACAAATTCAAGCGCGCTTAGGCCGAGCATTGAAGCAAATCGA
GAAAAACATCGGTGGTGATATGTTCCTCACTATGGCGCCTGAACACCCTTATGTGCAAGGTGGTATGGTTGCCTACAGTG
GAATTTGGGGCGCTTACATTCCGGTGATTAACGAAGTGCGCGATACGCTTGATATTCTGCATGTTCAGCTCTACAACAAC
GGTGGTTTACCTAACCCATACACACCGAGCGCGGCACCAGAAGGCTCTGTGGATATGATGGTCGCCCAATCAAAAATGTT
GATTGAAGGCTTCACTTTAGCCAACGGGACACGCTTTGAACCATTGCGTGATGACCAAGTAGCCATTGGTTTGCCATCAG
GTCCAAGTTCGGCAAATTCGGGTCAAGCTCCAACCCAAAATATCCTTGATGCGTTGGATTGTTTAACCAAAGGAACCCGC
TGCGGCACCATCAAACCAGCCTTTGCCTACCCCAATTATGCAGGCGTGATGACCTGGTCAATCAACTGGGATAAGCACGA
CGGCTTTAATTTTTCCAAACCGGTTGGGGATAAACTTAGCCAGATGAATAATGCTCAGTAA