Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00358  |
| Cognate effector | EFF01821 |
| Related T6SS (Type) | T6SS01168 (Type i1) |
| Strain | Vibrio cholerae C6706 |
| Replicon | chromosome |
| Sequence | Protein sequence (123 a.a.); Nucleotide sequence (369 bp) |
| Description | TsiV3 |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | GPY04_RS15915 (TsiV3) |
| Coordinates (Strand) | 291521..291889 (-) |
| NCBI ID | 446984039 |
| RefSeq | NZ_CP046845 |
| Uniprot ID | TSIV3_VIBCH, A0A0X1L1W1_VIBCO, A0A0H3AF63_VIBC3, A0A0K9UWF2_VIBCL, C3LUE8_VIBCM, A0A023PSF3_VIBCL |
| KEGG ID | vcx:VAA049_1784, vcm:VCM66_A0122, vch:VCA0124, vco:VC0395_0013 |
| PDB ID | 4NOO, 4NSR, 4NSO |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-3 | MNN |
| Transmembrane helix | 4-23 | LLSAYVTMLLILLSISGGAI |
| Inside | 24-122 | ASENCNDTSGVHQKILVCIQNEIAKSETQIRNNISSKSIDYGFPDDFYSKQRLAIHEKCMLYINVGGQRGELLMNQCELSMLQGLDIYIQQYIEDVDNS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9771 | pos: 24-25. AIA-SE. | MNNLLSAYVTMLLILLSISGGAIA |
Protein sequence: 123 a.a. .
>IMU00358 NZ_CP046845:c291889-291521 [Vibrio cholerae C6706]
MNNLLSAYVTMLLILLSISGGAIASENCNDTSGVHQKILVCIQNEIAKSETQIRNNISSKSIDYGFPDDFYSKQRLAIHE
KCMLYINVGGQRGELLMNQCELSMLQGLDIYIQQYIEDVDNS*
MNNLLSAYVTMLLILLSISGGAIASENCNDTSGVHQKILVCIQNEIAKSETQIRNNISSKSIDYGFPDDFYSKQRLAIHE
KCMLYINVGGQRGELLMNQCELSMLQGLDIYIQQYIEDVDNS*
Nucleotide sequence: 369 bp .
>IMU00358 NZ_CP046845:c291889-291521 [Vibrio cholerae C6706]
ATGAATAACTTGCTTTCTGCGTATGTAACTATGCTGTTGATATTATTAAGTATTAGTGGTGGCGCTATTGCTAGTGAAAA
CTGCAATGATACATCTGGAGTGCATCAAAAAATTCTAGTGTGTATCCAAAATGAGATAGCTAAGTCAGAGACTCAGATTA
GAAATAATATTTCTTCTAAGTCTATAGATTATGGCTTTCCTGATGATTTCTACAGCAAGCAAAGATTAGCTATTCATGAA
AAGTGTATGCTTTATATAAATGTCGGTGGCCAACGTGGTGAATTGCTAATGAACCAATGTGAACTTTCGATGCTACAAGG
CCTTGATATTTACATTCAACAGTATATAGAGGATGTTGATAATAGTTAG
ATGAATAACTTGCTTTCTGCGTATGTAACTATGCTGTTGATATTATTAAGTATTAGTGGTGGCGCTATTGCTAGTGAAAA
CTGCAATGATACATCTGGAGTGCATCAAAAAATTCTAGTGTGTATCCAAAATGAGATAGCTAAGTCAGAGACTCAGATTA
GAAATAATATTTCTTCTAAGTCTATAGATTATGGCTTTCCTGATGATTTCTACAGCAAGCAAAGATTAGCTATTCATGAA
AAGTGTATGCTTTATATAAATGTCGGTGGCCAACGTGGTGAATTGCTAATGAACCAATGTGAACTTTCGATGCTACAAGG
CCTTGATATTTACATTCAACAGTATATAGAGGATGTTGATAATAGTTAG