Detailed information of effector


Summary

Effector ID EFF01819 experimental
Effector type -
Cognate immunity protein -
Related T6SS (Type) T6SS01168 experimental (Type i1)
Strain Vibrio cholerae C6706
Replicon chromosome
Sequence Protein sequence (1164 a.a.); Nucleotide sequence (3492 bp)
Description VgrG-1 ACD is specifically necessary for the increased amplitude of intestinal contractions observed in fish monocolonized with the T6SS+ strain
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) GPY04_RS04820 (VgrG-1)
Coordinates (Strand) 1063272..1066763 (+)
NCBI ID 446134261
RefSeq NZ_CP046844
Uniprot ID VGRG1_VIBCH, A0A0H3Q601_VIBCL, C3LMB1_VIBCM
KEGG ID vch:VC1416, vcm:VCM66_1371
PDB ID 4GQK
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ACD PF16671.7 1.6e-238 726..1111
Phage_GPD PF05954.13 3.9e-77 41..334
Phage_base_V PF04717.14 1.1e-13 392..458
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1163 MATLAYSIEVEGLEDETLVVRGFHGQESLSNSVFLGQACYGFRYEVQLASRVSNLTAEQMVDKRAELKLYRNSQLVQRVHGIVRAFSQGDIGHHHTFYQLTLVPALERLSLRHNSRIFQKQTVPEILSILLQEMGINDYAFALKRDGVQREFCVQYRESDIDFLHRLAAEEGLVYSFVHEAGKHTLYFSDASDSLSKLPEPIPYNALVGGAIDTPYIHGLTYRTQAEVSEVQLKDYSFKKPAYSFLQTVQGTELDYQQTRYQHFDAPGRYKDDVNGAAFSQIRLDYLRRHAHTATGQSNEPLLRAGYKFDLQEHLDPAMNRDWVVVSINHQGEQPQALQEDGGSGATTYSNQFSLIPGHLHWRAEPQPKPQVDGPMIATVVGPEGEEIFCDEHGRVKIHFPWDRYSNGNEQSSCWVRVSQGWAGSQYGFIAIPRIGHEVIVEFLNGDPDQPIITGRTYHATNTPPYTLPEHKTKTVLRTETHQGEGFNELSFEDQAGKEQIYLHAQKDFDGLIENDHTTVIRHDHHLTVENDQFTQIKHNQHLTVEWESREAVTGEQVLSIEGSLHVKTGKVWVNEAGTEIHVKAGQKVVIEAGSEITVKAGGSFVKVDPAGVHLSGALVNLNSGGSAGSGSGFGGAMPALPGGLEPAVALAPPQTISYQALLQAEQANVPAVKVCPLAAQEATPAVNSITPPPPPPIAPPMAPPQPIMNPQPTANAQPNLGRSTKATPDFPTHFPKSSIGIENELAGLVVAMPANSAQKFGYVKSAQGDALFMLTKDMNQGSYQRPPSLQDGKNYQNWQTHTVELVSYPCEMDDKAAVETRKQAMLWLATHFTTHIDQSNHQPLAPIQSEDGRFVIEITNAKHVIAAGNGISAESQGQTITMTPSGQQATVGVAAKGFGTSATPELRLLESAPWYQKSLKSQFASLTSAENLDDKELAANVFAYLTSIYLKTAELAKKFGIYINEWDPMSEQITPNANGLTDPKVKNAWEILPRTKPSKIVEILSKSDAKAVMKHIKPQLQSRYSESLSKNVFQYFQDGGEVAGHGINNATVGDKHSPELAILFEFRTVPNELQSYLPKTESTTKSEVKLLDQFDPMKRKTVIQQVESLVQNSGDAFDKWYQSYRDSMNQPPVKNAKKIASANQKAQWVKEHNPQEWQRIIA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9832 - -


  Protein sequence: 1164 a.a.    .

>EFF01819 NZ_CP046844:1063272-1066763 (Unknown) [Vibrio cholerae C6706]
MATLAYSIEVEGLEDETLVVRGFHGQESLSNSVFLGQACYGFRYEVQLASRVSNLTAEQMVDKRAELKLYRNSQLVQRVH
GIVRAFSQGDIGHHHTFYQLTLVPALERLSLRHNSRIFQKQTVPEILSILLQEMGINDYAFALKRDGVQREFCVQYRESD
IDFLHRLAAEEGLVYSFVHEAGKHTLYFSDASDSLSKLPEPIPYNALVGGAIDTPYIHGLTYRTQAEVSEVQLKDYSFKK
PAYSFLQTVQGTELDYQQTRYQHFDAPGRYKDDVNGAAFSQIRLDYLRRHAHTATGQSNEPLLRAGYKFDLQEHLDPAMN
RDWVVVSINHQGEQPQALQEDGGSGATTYSNQFSLIPGHLHWRAEPQPKPQVDGPMIATVVGPEGEEIFCDEHGRVKIHF
PWDRYSNGNEQSSCWVRVSQGWAGSQYGFIAIPRIGHEVIVEFLNGDPDQPIITGRTYHATNTPPYTLPEHKTKTVLRTE
THQGEGFNELSFEDQAGKEQIYLHAQKDFDGLIENDHTTVIRHDHHLTVENDQFTQIKHNQHLTVEWESREAVTGEQVLS
IEGSLHVKTGKVWVNEAGTEIHVKAGQKVVIEAGSEITVKAGGSFVKVDPAGVHLSGALVNLNSGGSAGSGSGFGGAMPA
LPGGLEPAVALAPPQTISYQALLQAEQANVPAVKVCPLAAQEATPAVNSITPPPPPPIAPPMAPPQPIMNPQPTANAQPN
LGRSTKATPDFPTHFPKSSIGIENELAGLVVAMPANSAQKFGYVKSAQGDALFMLTKDMNQGSYQRPPSLQDGKNYQNWQ
THTVELVSYPCEMDDKAAVETRKQAMLWLATHFTTHIDQSNHQPLAPIQSEDGRFVIEITNAKHVIAAGNGISAESQGQT
ITMTPSGQQATVGVAAKGFGTSATPELRLLESAPWYQKSLKSQFASLTSAENLDDKELAANVFAYLTSIYLKTAELAKKF
GIYINEWDPMSEQITPNANGLTDPKVKNAWEILPRTKPSKIVEILSKSDAKAVMKHIKPQLQSRYSESLSKNVFQYFQDG
GEVAGHGINNATVGDKHSPELAILFEFRTVPNELQSYLPKTESTTKSEVKLLDQFDPMKRKTVIQQVESLVQNSGDAFDK
WYQSYRDSMNQPPVKNAKKIASANQKAQWVKEHNPQEWQRIIA*

  Nucleotide sequence: 3492 bp    .

>EFF01819 NZ_CP046844:1063272-1066763 (Unknown) [Vibrio cholerae C6706]
ATGGCGACATTAGCGTACAGCATTGAGGTGGAAGGGCTGGAAGATGAGACTCTGGTGGTCAGAGGCTTCCACGGGCAGGA
GTCACTCTCTAATAGTGTTTTTTTAGGGCAAGCCTGTTACGGGTTTCGCTACGAAGTGCAACTGGCGAGTCGGGTATCAA
ACCTCACCGCCGAGCAGATGGTGGATAAGCGTGCCGAGCTCAAGCTTTACCGCAACTCACAGTTGGTGCAGCGCGTGCAC
GGCATTGTGCGTGCTTTCAGCCAAGGCGATATCGGCCATCACCACACCTTCTACCAACTCACTTTAGTCCCTGCCTTAGA
GCGCTTATCCCTGCGTCATAACAGCCGCATTTTCCAAAAGCAGACTGTCCCTGAGATTCTCTCTATCTTGCTGCAAGAGA
TGGGCATCAACGATTACGCTTTTGCGCTCAAACGTGATGGCGTGCAGCGTGAGTTTTGTGTGCAGTACCGCGAAAGCGAT
ATCGACTTTCTGCACCGCCTCGCCGCTGAAGAAGGTTTGGTGTACAGCTTTGTCCATGAAGCGGGCAAACATACCCTCTA
TTTCAGTGATGCCAGTGATAGCCTAAGCAAACTGCCGGAGCCTATCCCATACAACGCTTTAGTCGGTGGCGCGATAGATA
CCCCGTATATCCACGGCTTAACCTATCGCACCCAAGCAGAAGTGAGCGAAGTTCAGCTCAAAGACTACAGCTTCAAAAAG
CCAGCCTACTCCTTTTTGCAAACCGTGCAGGGTACTGAGCTGGATTATCAGCAAACGCGCTACCAACATTTTGATGCGCC
GGGGCGCTACAAAGACGATGTGAACGGCGCCGCCTTTAGCCAAATCCGTTTAGACTACCTGCGCCGCCATGCCCATACCG
CAACAGGGCAGAGCAACGAGCCGCTGCTGCGTGCCGGCTATAAGTTTGACCTGCAAGAGCACCTTGACCCCGCGATGAAC
CGCGATTGGGTGGTAGTGTCGATTAACCACCAAGGTGAGCAGCCGCAAGCGCTACAAGAAGACGGTGGCAGCGGCGCTAC
TACTTACAGCAATCAGTTCAGCTTAATTCCGGGGCATCTTCACTGGCGCGCCGAGCCACAACCCAAACCGCAAGTCGATG
GTCCAATGATTGCCACGGTCGTCGGTCCTGAAGGCGAAGAAATCTTCTGTGATGAACATGGGCGGGTGAAAATCCATTTC
CCGTGGGACCGTTACTCGAACGGTAACGAGCAAAGCTCCTGTTGGGTACGGGTGTCGCAAGGTTGGGCGGGCAGTCAGTA
TGGCTTTATCGCTATTCCGCGCATCGGCCATGAAGTGATTGTAGAGTTTTTAAACGGTGACCCAGACCAACCGATTATCA
CTGGCCGCACTTATCATGCGACCAACACGCCGCCATACACCTTGCCTGAGCACAAAACCAAAACCGTGCTGCGCACCGAG
ACGCACCAAGGGGAGGGCTTTAACGAGCTCAGTTTTGAAGACCAAGCGGGCAAAGAGCAAATCTACCTGCATGCGCAGAA
AGATTTTGATGGTTTGATTGAAAACGACCACACCACGGTCATTCGTCACGACCATCACCTGACCGTTGAAAACGACCAAT
TTACCCAGATTAAGCACAACCAACACCTGACGGTGGAGTGGGAAAGTCGAGAAGCGGTAACGGGCGAGCAAGTGCTGAGT
ATTGAAGGCTCGCTGCATGTGAAAACAGGCAAAGTTTGGGTCAATGAAGCGGGTACAGAGATCCATGTAAAAGCGGGACA
GAAAGTGGTGATTGAAGCGGGCTCCGAGATTACCGTTAAAGCGGGCGGCAGCTTTGTGAAAGTCGATCCGGCTGGGGTGC
ATCTGTCCGGTGCTTTGGTCAATCTCAACTCTGGCGGCAGCGCTGGTAGTGGTAGCGGCTTTGGCGGTGCAATGCCCGCC
TTACCGGGCGGCTTAGAACCCGCCGTGGCACTGGCTCCACCGCAAACCATCTCTTACCAAGCGTTGCTACAGGCCGAGCA
AGCGAATGTTCCTGCGGTGAAGGTGTGTCCTTTAGCCGCTCAAGAAGCAACGCCTGCGGTGAATAGTATCACGCCGCCTC
CACCACCACCGATTGCTCCGCCAATGGCCCCACCACAACCAATAATGAATCCACAGCCAACCGCTAACGCGCAGCCTAAT
CTTGGAAGATCCACTAAGGCAACGCCGGATTTTCCAACACATTTCCCGAAGTCATCCATTGGCATTGAGAATGAGTTAGC
TGGTCTAGTGGTGGCTATGCCAGCCAACTCGGCACAGAAATTTGGCTACGTGAAAAGTGCACAAGGTGATGCGCTGTTTA
TGTTAACGAAAGACATGAACCAAGGTTCGTATCAGCGGCCACCTTCATTACAAGATGGAAAAAATTATCAGAATTGGCAG
ACGCATACAGTAGAGTTGGTGAGCTACCCTTGTGAAATGGACGATAAAGCTGCCGTTGAAACACGCAAACAAGCCATGTT
GTGGTTAGCAACCCACTTTACTACGCATATCGACCAGTCCAATCATCAACCATTAGCTCCTATTCAAAGTGAAGACGGAC
GCTTTGTTATCGAAATCACTAATGCGAAGCATGTGATTGCTGCGGGCAATGGGATTTCTGCAGAATCTCAAGGTCAAACA
ATCACGATGACACCAAGTGGACAACAAGCCACAGTGGGTGTTGCTGCTAAAGGTTTCGGGACGAGTGCAACGCCAGAATT
AAGGTTACTTGAATCGGCACCTTGGTATCAGAAATCACTCAAGAGCCAATTTGCATCTCTAACGAGCGCAGAAAACTTGG
ATGATAAAGAGTTAGCTGCAAACGTATTTGCTTATCTGACGTCTATTTATTTAAAAACAGCAGAGCTAGCGAAGAAGTTT
GGTATTTACATCAATGAATGGGACCCCATGTCGGAGCAAATTACTCCAAATGCGAATGGATTAACGGATCCAAAAGTAAA
AAATGCATGGGAAATATTGCCACGTACAAAACCAAGCAAAATAGTAGAAATACTCAGTAAGAGTGATGCCAAAGCGGTTA
TGAAACATATCAAGCCTCAATTGCAGTCTCGCTATTCTGAGAGTTTGTCGAAAAATGTATTTCAATATTTCCAAGATGGT
GGTGAAGTAGCCGGACACGGTATTAATAATGCGACGGTCGGTGATAAACATAGTCCGGAATTGGCGATTTTGTTTGAGTT
CAGAACTGTTCCGAATGAACTGCAAAGTTATTTGCCTAAAACAGAATCGACAACTAAGTCAGAAGTGAAGTTACTCGATC
AGTTTGATCCTATGAAGCGTAAAACAGTAATTCAACAAGTAGAATCGCTTGTGCAAAATAGTGGCGATGCATTTGATAAA
TGGTACCAAAGCTACCGAGACTCCATGAATCAACCTCCCGTTAAAAATGCGAAGAAAATTGCTTCCGCGAATCAAAAAGC
ACAATGGGTTAAAGAGCATAATCCTCAAGAATGGCAACGCATTATTGCTTAG