Detailed information    

insolico Bioinformatically predicted

Overview

Name   recA   Type   Machinery gene
Locus tag   EW035_RS05575 Genome accession   NZ_LR130552
Coordinates   1156763..1157824 (+) Length   353 a.a.
NCBI ID   WP_000963143.1    Uniprot ID   P0A7G7
Organism   Escherichia coli strain MS14386 isolate MS14386     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1151763..1162824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW035_RS05545 srlD 1152016..1152795 (-) 780 WP_001077358.1 sorbitol-6-phosphate dehydrogenase -
  EW035_RS05550 srlB 1152799..1153170 (-) 372 WP_000216210.1 PTS glucitol/sorbitol transporter subunit IIA -
  EW035_RS05555 srlE 1153181..1154140 (-) 960 WP_000148875.1 PTS glucitol/sorbitol transporter subunit IIB -
  EW035_RS05560 srlA 1154137..1154700 (-) 564 WP_000573321.1 PTS glucitol/sorbitol transporter subunit IIC -
  EW035_RS05565 mltB 1154956..1156041 (+) 1086 WP_023156038.1 lytic murein transglycosylase B -
  EW035_RS05570 pncC 1156186..1156683 (+) 498 WP_000132231.1 nicotinamide-nucleotide amidase -
  EW035_RS05575 recA 1156763..1157824 (+) 1062 WP_000963143.1 recombinase RecA Machinery gene
  EW035_RS05580 recX 1158067..1158567 (+) 501 WP_000140506.1 recombination regulator RecX -
  EW035_RS05585 alaS 1158695..1161325 (+) 2631 WP_024166692.1 alanine--tRNA ligase -
  EW035_RS05590 csrA 1161560..1161745 (+) 186 WP_000906486.1 carbon storage regulator CsrA -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37973.37 Da        Isoelectric Point: 4.8171

>NTDB_id=996115 EW035_RS05575 WP_000963143.1 1156763..1157824(+) (recA) [Escherichia coli strain MS14386 isolate MS14386]
MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQV
IAAAQREGKTCAFIDAEHALDPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVG
SETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEI
EKKVRELLLSNPNSTPDFSVDDSEGVAETNEDF

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=996115 EW035_RS05575 WP_000963143.1 1156763..1157824(+) (recA) [Escherichia coli strain MS14386 isolate MS14386]
ATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATTGAGAAACAATTTGGTAAAGGCTCCAT
CATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATATCGCGCTTGGGG
CAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCGGAATCTTCCGGTAAAACCACGCTGACGCTGCAGGTG
ATCGCCGCAGCGCAGCGTGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAA
ACTGGGCGTCGATATCGATAACCTGCTGTGCTCCCAGCCGGACACCGGCGAGCAGGCACTGGAAATCTGTGACGCCCTGG
CGCGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCACTGACGCCGAAAGCGGAAATCGAAGGCGAAATC
GGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTGAAGCAGTCCAA
CACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTA
ACGCGCTGAAATTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGT
AGCGAAACCCGCGTGAAAGTGGTGAAGAACAAAATCGCTGCGCCGTTTAAACAGGCTGAATTCCAGATCCTCTACGGCGA
AGGTATCAACTTCTACGGCGAACTGGTTGACCTGGGCGTAAAAGAGAAGCTGATCGAGAAAGCAGGCGCGTGGTACAGCT
ACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCAACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAGATC
GAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGC
AGAAACTAACGAAGATTTTTAA

Domains

Predicted by InterProScan.

recA bacterial DNA recombination protein (RecA_N)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recA Vibrio cholerae strain A1552

84.29

93.768

0.79
  recA Vibrio cholerae O1 biovar El Tor strain E7946

84.29

93.768

0.79
  recA Pseudomonas stutzeri DSM 10701

74.184

95.467

0.708
  recA Acinetobacter baumannii D1279779

74.085

92.918

0.688
  recA Acinetobacter nosocomialis M2

73.78

92.918

0.686
  recA Acinetobacter baylyi ADP1

73.78

92.918

0.686
  recA Glaesserella parasuis strain SC1401

69.452

98.3

0.683
  recA Neisseria gonorrhoeae MS11

69.018

92.351

0.637
  recA Neisseria gonorrhoeae strain FA1090

69.018

92.351

0.637
  recA Ralstonia pseudosolanacearum GMI1000

70.159

89.235

0.626
  recA Streptococcus mitis NCTC 12261

60.286

99.15

0.598
  recA Streptococcus mitis SK321

60.286

99.15

0.598
  recA Helicobacter pylori strain NCTC11637

61.721

95.467

0.589
  recA Streptococcus thermophilus LMG 18311

58.263

100

0.589
  recA Helicobacter pylori 26695

61.424

95.467

0.586
  recA Streptococcus pneumoniae Rx1

63.467

91.501

0.581
  recA Streptococcus pneumoniae R36A

63.467

91.501

0.581
  recA Streptococcus pneumoniae D39

63.467

91.501

0.581
  recA Streptococcus pneumoniae R6

63.467

91.501

0.581
  recA Streptococcus pneumoniae TIGR4

63.467

91.501

0.581
  recA Staphylococcus aureus strain ATCC 12600

62.577

92.351

0.578
  recA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

62.654

91.785

0.575
  recA Riemerella anatipestifer ATCC 11845 = DSM 15868

60.661

94.334

0.572
  recA Streptococcus thermophilus LMD-9

62.154

92.068

0.572
  recA Streptococcus pyogenes NZ131

62.154

92.068

0.572
  recA Streptococcus mutans UA159

62.154

92.068

0.572
  recA Bacillus subtilis subsp. subtilis str. 168

62.305

90.935

0.567
  recA Latilactobacillus sakei subsp. sakei 23K

61.538

92.068

0.567
  recA Lactococcus lactis subsp. cremoris KW2

61.61

91.501

0.564
  recA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

61.059

90.935

0.555