Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L6424_RS09530 Genome accession   NZ_AP025277
Coordinates   1993200..1994339 (-) Length   379 a.a.
NCBI ID   WP_236766716.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain NUITM-VA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1988200..1999339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6424_RS09510 (NUITMVA1_18140) feoB 1988394..1990667 (+) 2274 WP_124252919.1 Fe(2+) transporter permease subunit FeoB -
  L6424_RS09515 (NUITMVA1_18150) - 1990664..1990906 (+) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  L6424_RS09520 (NUITMVA1_18160) - 1991030..1991509 (-) 480 WP_236766714.1 WbuC family cupin fold metalloprotein -
  L6424_RS09525 (NUITMVA1_18170) glgC 1991645..1992916 (-) 1272 WP_236766715.1 glucose-1-phosphate adenylyltransferase -
  L6424_RS09530 (NUITMVA1_18180) pilU 1993200..1994339 (-) 1140 WP_236766716.1 type IVa pilus ATPase TapW Machinery gene
  L6424_RS09535 (NUITMVA1_18190) - 1994400..1994756 (-) 357 WP_005302630.1 DMT family protein -
  L6424_RS09540 (NUITMVA1_18200) - 1994870..1995601 (+) 732 WP_029300033.1 substrate-binding periplasmic protein -
  L6424_RS09545 (NUITMVA1_18210) btuC 1995702..1996709 (+) 1008 WP_236766717.1 vitamin B12 ABC transporter permease BtuC -
  L6424_RS09550 (NUITMVA1_18220) - 1996696..1997481 (+) 786 WP_236766718.1 ATP-binding cassette domain-containing protein -
  L6424_RS09555 (NUITMVA1_18230) aac(6') 1997503..1997961 (-) 459 WP_236766719.1 aminoglycoside 6'-N-acetyltransferase -
  L6424_RS09560 (NUITMVA1_18240) - 1997968..1999032 (-) 1065 WP_236766720.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42206.28 Da        Isoelectric Point: 6.6010

>NTDB_id=90943 L6424_RS09530 WP_236766716.1 1993200..1994339(-) (pilU) [Aeromonas hydrophila strain NUITM-VA1]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSGGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=90943 L6424_RS09530 WP_236766716.1 1993200..1994339(-) (pilU) [Aeromonas hydrophila strain NUITM-VA1]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAATGGCGGCCTGCGTCCGCTCAGTGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
TGCCCTGAAAAACACCCTGCGTCAGGCACCGGACGTGATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAGCATG
CACTGGCCTTCTCCGAGACCGGCCACCTGGCGATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCTCAGCTGCTCAACGACCTTGGCAACAACCTCAAAGCGTTCGTCTCCCAGCG
GCTGGTCAAAACCAGCGGCGGTGGTCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATCA
AACGCGGCGAGTTCGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTAGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAAAAGGGCCAGATCGCGAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii D1279779

41.317

88.127

0.364
  pilT Acinetobacter baumannii strain A118

41.317

88.127

0.364
  pilT Acinetobacter nosocomialis M2

41.317

88.127

0.364

Multiple sequence alignment