Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Atep_RS03045 Genome accession   NZ_AP024563
Coordinates   649213..650367 (-) Length   384 a.a.
NCBI ID   WP_213380219.1    Uniprot ID   -
Organism   Allochromatium tepidum strain NZ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644213..655367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Atep_RS03030 (Atep_05970) - 644548..646785 (+) 2238 WP_213380216.1 methyl-accepting chemotaxis protein -
  Atep_RS03035 (Atep_05980) - 646841..648076 (+) 1236 WP_213380217.1 PP2C family protein-serine/threonine phosphatase -
  Atep_RS03040 (Atep_05990) - 648130..649203 (+) 1074 WP_213380218.1 HD domain-containing phosphohydrolase -
  Atep_RS03045 (Atep_06000) pilU 649213..650367 (-) 1155 WP_213380219.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Atep_RS03050 (Atep_06010) pilT 650399..651436 (-) 1038 WP_213380220.1 type IV pilus twitching motility protein PilT Machinery gene
  Atep_RS03055 (Atep_06020) purE 651514..652029 (-) 516 WP_213380221.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  Atep_RS03060 (Atep_06030) purK 652026..653180 (-) 1155 WP_213380222.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  Atep_RS03065 (Atep_06040) hemF 653344..654264 (+) 921 WP_213380223.1 oxygen-dependent coproporphyrinogen oxidase -
  Atep_RS03070 (Atep_06050) - 654273..655349 (-) 1077 WP_213380224.1 glycosyltransferase -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 43047.42 Da        Isoelectric Point: 6.7064

>NTDB_id=86011 Atep_RS03045 WP_213380219.1 649213..650367(-) (pilU) [Allochromatium tepidum strain NZ]
MDLKRALEQLLNALVEKKGSDLFITAGRPPTVKIDGTLYPATKQPLTAEQARSMVYDIMNERQRAEFEDTKECQFALDRA
PLGRFRVSAFVQRDAVGMVLRRIETRIPTLEELKLPSVLRDLAMTKRGLVIFVGATGTGKSTSLAALIGYRNHHSSGHII
TVEDPIEYVHEHDGCIITQREVGVDTESFEVALRNTLRQAPDVILIGEIRTRQTMEYAITFAETGHLVLATLHANNANQA
MDRIINFFPEESRKQMLMDLSLNLKGIVAQQLAPRKNGQGRRAVIEILLNTPLAADLIRMGEVHKLKDLMKRSGEQGMIT
FDQALFNLYQEGEISYEDALKYADSANEVRLMIKLQSGVTPSSATDQSIDAMSLVRDEDPRNKR

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=86011 Atep_RS03045 WP_213380219.1 649213..650367(-) (pilU) [Allochromatium tepidum strain NZ]
ATGGATCTGAAGCGCGCGCTGGAACAACTCTTGAACGCCCTGGTCGAGAAAAAGGGCTCGGACCTCTTCATCACCGCCGG
CCGGCCGCCGACCGTCAAGATCGACGGCACCCTCTACCCCGCCACCAAGCAACCGCTCACGGCCGAGCAGGCGCGCAGCA
TGGTCTACGACATCATGAACGAGCGCCAGCGCGCCGAGTTCGAGGACACCAAGGAGTGTCAGTTCGCGCTCGACCGCGCC
CCGCTCGGACGCTTCCGTGTCAGTGCCTTCGTCCAGCGCGACGCGGTCGGCATGGTGCTGCGGCGCATCGAGACCCGCAT
CCCGACCCTGGAGGAACTCAAGCTCCCCTCGGTGCTGCGCGATCTGGCCATGACCAAGCGCGGTCTGGTGATCTTCGTCG
GCGCCACCGGCACGGGTAAGTCGACCTCGCTCGCGGCCCTGATCGGCTATCGCAACCATCATAGCTCGGGACACATCATC
ACGGTCGAGGATCCGATCGAGTACGTCCACGAACACGACGGCTGCATCATCACTCAGCGCGAGGTCGGGGTGGACACCGA
GTCCTTCGAGGTCGCCCTGCGCAACACATTGCGGCAGGCCCCGGACGTCATCCTGATCGGCGAGATCCGCACCCGCCAGA
CCATGGAATACGCCATCACCTTCGCTGAGACCGGCCATCTGGTGCTCGCCACCCTGCACGCCAACAACGCCAATCAGGCG
ATGGATCGCATCATCAACTTCTTCCCCGAGGAATCGCGCAAGCAGATGCTCATGGACCTCTCGCTCAACCTCAAGGGCAT
CGTCGCCCAGCAGCTGGCGCCGCGCAAGAACGGCCAGGGCCGGCGCGCGGTGATCGAGATCCTGCTCAACACGCCGCTGG
CCGCCGACCTCATCCGCATGGGCGAGGTGCACAAGCTCAAGGATCTGATGAAGCGTTCGGGCGAACAGGGCATGATCACC
TTCGATCAGGCGCTGTTCAATCTCTATCAGGAAGGCGAGATCAGCTACGAGGATGCCCTGAAGTACGCCGACTCGGCCAA
CGAGGTACGGCTGATGATCAAGCTCCAGAGCGGCGTGACGCCGAGCAGCGCGACGGATCAGAGCATCGACGCCATGAGTC
TGGTCCGGGACGAAGACCCCAGGAACAAACGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.845

97.396

0.651
  pilU Acinetobacter baylyi ADP1

62.536

90.365

0.565
  pilU Vibrio cholerae strain A1552

59.504

94.531

0.562
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.056

93.75

0.404
  pilT Legionella pneumophila strain Lp02

43.544

86.719

0.378
  pilT Legionella pneumophila strain ERS1305867

43.544

86.719

0.378
  pilT Acinetobacter baylyi ADP1

41.888

88.281

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

86.458

0.362
  pilT Vibrio cholerae strain A1552

41.867

86.458

0.362

Multiple sequence alignment