Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JUH08_RS02760 Genome accession   NZ_AP024354
Coordinates   608682..609746 (+) Length   354 a.a.
NCBI ID   WP_061903206.1    Uniprot ID   A0AA42MZ32
Organism   Aquipseudomonas alcaligenes strain KAM426     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 603682..614746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JUH08_RS02750 (KAM426_05560) - 604753..606021 (+) 1269 WP_203790383.1 malic enzyme-like NAD(P)-binding protein -
  JUH08_RS02755 (KAM426_05570) - 606083..608497 (-) 2415 WP_373869750.1 penicillin-binding protein 1A -
  JUH08_RS02760 (KAM426_05580) pilM 608682..609746 (+) 1065 WP_061903206.1 pilus assembly protein PilM Machinery gene
  JUH08_RS02765 (KAM426_05590) - 609746..610372 (+) 627 WP_203790385.1 PilN domain-containing protein -
  JUH08_RS02770 (KAM426_05600) pilO 610369..611001 (+) 633 WP_203790394.1 type 4a pilus biogenesis protein PilO -
  JUH08_RS02775 (KAM426_05610) pilP 610998..611525 (+) 528 WP_021698831.1 type 4a pilus biogenesis lipoprotein PilP -
  JUH08_RS02780 (KAM426_05620) pilQ 611578..613650 (+) 2073 WP_203790401.1 type IV pilus secretin PilQ Machinery gene
  JUH08_RS02785 (KAM426_05630) aroK 613653..614171 (+) 519 WP_021698829.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37952.32 Da        Isoelectric Point: 4.4244

>NTDB_id=84928 JUH08_RS02760 WP_061903206.1 608682..609746(+) (pilM) [Aquipseudomonas alcaligenes strain KAM426]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALARVLSKAKTSVRTAAV
AVAGSAVITKTIEMEAGLNDDELENQLKIEADQYIPYPLEEVAIDFEVQGLSPRNPERVEVLLAACRKENVEVREAALGL
AGVTAKVVDVEAYALERAYGLLESQLGGSVDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYESEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRMIQQKIGTPTLVANP
FADMALGSKVNAGALASDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=84928 JUH08_RS02760 WP_061903206.1 608682..609746(+) (pilM) [Aquipseudomonas alcaligenes strain KAM426]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACGCTTCTGGGGATCGACATCAGTTCGACCTCGGTCAAGCTCCTTGAATT
GAGTCGCTCCGGAAGCCGCTACAAGGTAGAGGCCTATGCTGTCGAGCCGCTCCCACCCAATGCTGTTGTCGAAAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCTCTTGCGCGCGTTCTGAGCAAGGCGAAAACCAGCGTGCGTACTGCGGCGGTG
GCGGTTGCTGGCTCTGCGGTGATCACCAAGACCATCGAGATGGAAGCCGGTCTCAACGATGACGAGCTGGAGAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCATTGGAAGAGGTGGCTATCGACTTCGAGGTGCAGGGCCTTTCGCCGC
GCAACCCCGAACGAGTCGAAGTCCTGCTGGCTGCCTGCCGTAAAGAGAACGTCGAGGTTCGCGAGGCGGCTCTTGGGCTA
GCCGGTGTGACGGCCAAGGTTGTCGACGTGGAGGCTTATGCGCTCGAGCGCGCCTATGGCCTGCTCGAGTCCCAGCTGGG
CGGTAGCGTCGATGAGCTGACGGTTGCTGTGGTCGACATCGGTGCCACCATGACCACCCTGAGCGTGCTGCACAATGGCC
GCACGATCTACACCCGGGAGCAGCTGTTCGGTGGGCGTCAGCTGACCGAAGAGATTCAGCGCCGCTACGGTCTCTCCGTC
GAGGAGGCCGGTCTGGCCAAGAAACAGGGTGGCCTGCCGGATGACTACGAAAGCGAAGTGCTGCAGCCCTTCAAGGAAGC
GGTCGTGCAGCAGGTTTCGCGTTCGCTGCAATTCTTCTTCGCGGCCGGTCAGTTCAACGATGTCGACTACATCCTGCTGG
CAGGTGGTACGGCGTCGATTCCCGATCTGGATCGGATGATTCAACAGAAGATTGGTACTCCTACCCTGGTGGCCAACCCC
TTCGCCGACATGGCGCTGGGAAGCAAGGTCAATGCCGGAGCCCTGGCTAGCGATGCCCCGTCCTTGATGATTGCCTGTGG
CCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

45.763

100

0.458

Multiple sequence alignment