Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PWK10_RS08350 Genome accession   NZ_CP118396
Coordinates   1214962..1215453 (-) Length   163 a.a.
NCBI ID   WP_342455737.1    Uniprot ID   -
Organism   Caloramator sp. Dgby_cultured_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1209962..1220453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWK10_RS08320 (PWK10_08320) - 1210866..1211300 (-) 435 WP_274767976.1 hypothetical protein -
  PWK10_RS08325 (PWK10_08325) - 1211476..1211637 (-) 162 WP_274767977.1 hypothetical protein -
  PWK10_RS08330 (PWK10_08330) - 1211768..1212506 (-) 739 Protein_1671 prepilin peptidase -
  PWK10_RS08335 (PWK10_08335) - 1212561..1212830 (-) 270 WP_274767978.1 type II secretion system F family protein -
  PWK10_RS08340 (PWK10_08340) - 1213628..1214666 (-) 1039 Protein_1673 type IV pilus twitching motility protein PilT -
  PWK10_RS08345 (PWK10_08345) - 1214696..1214956 (-) 261 WP_274767979.1 hypothetical protein -
  PWK10_RS08350 (PWK10_08350) pilB 1214962..1215453 (-) 492 WP_342455737.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  PWK10_RS08355 (PWK10_08355) - 1215435..1216085 (-) 651 WP_274767980.1 ATPase, T2SS/T4P/T4SS family -
  PWK10_RS08360 (PWK10_08360) - 1216130..1216465 (-) 336 WP_274767981.1 hypothetical protein -
  PWK10_RS08365 (PWK10_08365) - 1216411..1216737 (-) 327 WP_274767982.1 hypothetical protein -
  PWK10_RS18530 - 1216738..1216923 (-) 186 Protein_1679 shikimate dehydrogenase -
  PWK10_RS08375 (PWK10_08375) - 1217004..1217381 (-) 378 WP_342455738.1 HAD-IIIA family hydrolase -
  PWK10_RS08380 (PWK10_08380) sigK 1217567..1218247 (-) 681 Protein_1681 RNA polymerase sporulation sigma factor SigK -
  PWK10_RS08385 (PWK10_08385) udk 1218307..1218930 (-) 624 WP_274767983.1 uridine kinase -
  PWK10_RS08390 (PWK10_08390) - 1218923..1220153 (-) 1231 Protein_1683 U32 family peptidase -

Sequence

Protein


Download         Length: 163 a.a.        Molecular weight: 17755.96 Da        Isoelectric Point: 10.0030

>NTDB_id=792377 PWK10_RS08350 WP_342455737.1 1214962..1215453(-) (pilB) [Caloramator sp. Dgby_cultured_2]
MLLGALSKGNGIILVTGPTGSGKSSTLYSILKLLNKESLNVMTIEDPVEYSVDGINQINVNPKAGLNFATGLRAILRQDP
DVIMIGEIRDKETAEIAVRAAVTGHLVLSTFHTNDVFTTITRLLEMKIEPYLLASAINLIISQRLVKKFALIVKKNISLQ
ILK

Nucleotide


Download         Length: 492 bp        

>NTDB_id=792377 PWK10_RS08350 WP_342455737.1 1214962..1215453(-) (pilB) [Caloramator sp. Dgby_cultured_2]
ATTTTACTTGGTGCATTAAGCAAAGGTAATGGTATTATTTTAGTTACGGGTCCAACAGGTAGCGGTAAATCCTCGACCTT
ATATTCTATTTTAAAGTTATTAAATAAGGAAAGCCTTAATGTTATGACGATTGAAGATCCAGTTGAATATTCTGTTGATG
GTATTAATCAAATAAATGTTAATCCAAAGGCAGGGCTCAATTTTGCAACGGGACTTAGGGCTATTTTAAGGCAGGATCCG
GATGTTATAATGATAGGTGAGATAAGAGATAAGGAAACTGCAGAGATTGCAGTTAGAGCAGCAGTTACAGGACATTTAGT
TCTTTCTACGTTTCACACGAATGATGTTTTTACAACTATAACTAGATTACTAGAAATGAAAATAGAGCCATATTTATTAG
CTTCAGCAATTAATCTTATAATTTCACAACGACTTGTAAAAAAATTTGCCCTAATTGTAAAGAAGAATATAAGCCTTCAA
ATTTTGAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(4-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

59.06

91.411

0.54
  pilB Vibrio cholerae strain A1552

56.494

94.479

0.534
  pilF Thermus thermophilus HB27

60.839

87.73

0.534
  pilB Vibrio parahaemolyticus RIMD 2210633

55.484

95.092

0.528
  pilB Acinetobacter baylyi ADP1

53.459

97.546

0.521
  pilB Vibrio campbellii strain DS40M4

54.194

95.092

0.515
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.5

98.16

0.515
  pilB Acinetobacter baumannii D1279779

54.545

94.479

0.515
  pilF Neisseria gonorrhoeae MS11

52.229

96.319

0.503
  ctsE Campylobacter jejuni subsp. jejuni 81-176

56.25

88.344

0.497
  pilB Haemophilus influenzae Rd KW20

55.862

88.957

0.497
  pilB Glaesserella parasuis strain SC1401

54.362

91.411

0.497
  pilB Haemophilus influenzae 86-028NP

55.479

89.571

0.497
  comGA Staphylococcus aureus MW2

54.225

87.117

0.472
  comGA Staphylococcus aureus N315

54.225

87.117

0.472
  comGA Bacillus subtilis subsp. subtilis str. 168

51.37

89.571

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

53.623

84.663

0.454
  pilT Pseudomonas aeruginosa PAK

42.953

91.411

0.393
  comGA Latilactobacillus sakei subsp. sakei 23K

42.953

91.411

0.393
  pilT Pseudomonas stutzeri DSM 10701

42.953

91.411

0.393
  pilT Acinetobacter baumannii D1279779

42.282

91.411

0.387
  pilT Acinetobacter baylyi ADP1

42.282

91.411

0.387
  pilT Acinetobacter nosocomialis M2

42.282

91.411

0.387
  pilT Acinetobacter baumannii strain A118

42.282

91.411

0.387
  pilT Legionella pneumophila strain ERS1305867

40.523

93.865

0.38
  pilT Legionella pneumophila strain Lp02

40.523

93.865

0.38
  pilT Neisseria meningitidis 8013

41.892

90.798

0.38
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.194

76.074

0.374
  pilT Neisseria gonorrhoeae MS11

41.216

90.798

0.374
  pilT Vibrio cholerae strain A1552

40.268

91.411

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.268

91.411

0.368