Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   POF63_RS08385 Genome accession   NZ_CP116708
Coordinates   1633290..1633781 (-) Length   163 a.a.
NCBI ID   WP_000609586.1    Uniprot ID   A0AAD2WUV1
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context


Location: 1628290..1638781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF63_RS08370 (POF63_08370) - 1631196..1631867 (-) 672 WP_000174848.1 DUF1129 family protein -
  POF63_RS08375 (POF63_08375) - 1631892..1632836 (-) 945 WP_000812124.1 magnesium transporter CorA family protein -
  POF63_RS08380 (POF63_08380) rpsR 1633006..1633245 (-) 240 WP_000068665.1 30S ribosomal protein S18 -
  POF63_RS08385 (POF63_08385) ssb 1633290..1633781 (-) 492 WP_000609586.1 single-stranded DNA-binding protein Machinery gene
  POF63_RS08390 (POF63_08390) rpsF 1633793..1634080 (-) 288 WP_001151773.1 30S ribosomal protein S6 -
  POF63_RS08395 (POF63_08395) mutY 1635143..1636297 (+) 1155 WP_162473108.1 A/G-specific adenine glycosylase -
  POF63_RS08400 (POF63_08400) - 1636474..1637067 (+) 594 WP_000402394.1 helix-turn-helix transcriptional regulator -
  POF63_RS08405 (POF63_08405) trxA 1637113..1637445 (-) 333 WP_001932060.1 thioredoxin -
  POF63_RS08410 (POF63_08410) - 1637508..1638008 (-) 501 WP_000446809.1 phosphatase PAP2 family protein -

Sequence


Protein


Download         Length: 163 a.a.        Molecular weight: 18155.77 Da        Isoelectric Point: 4.9119

>NTDB_id=779334 POF63_RS08385 WP_000609586.1 1633290..1633781(-) (ssb) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGEREADFINCVIWRQQAENLANWAKKGALVGITGRIQTR
NYENQQGQRVYVTEVVAESFQLLESRATREGGSSNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
LPF

Nucleotide


Download         Length: 492 bp        

>NTDB_id=779334 POF63_RS08385 WP_000609586.1 1633290..1633781(-) (ssb) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGATTAATAATGTAGTACTTGTAGGTCGCATGACCCGTGATGCAGAACTTCGTTATACACCAAGTAATCAAGCGGTAGC
CACTTTCTCACTTGCAGTTAATCGTAATTTTAAAAATCAATCTGGCGAACGTGAGGCTGATTTTATTAACTGTGTTATTT
GGCGCCAACAAGCTGAAAACTTGGCTAACTGGGCAAAAAAAGGTGCTTTGGTTGGAATTACAGGTCGTATCCAAACGCGT
AATTATGAAAACCAACAAGGTCAACGTGTCTATGTAACAGAAGTTGTTGCGGAAAGTTTCCAATTATTGGAAAGTCGTGC
TACACGAGAAGGTGGTTCATCTAACTCTTATAATAACGGTGGCTATAACAATGCTCCGTCAAATAATAGTTATTCAGCTT
CTTCTCAACAAACGCCTAATTTTAGTCGTGATGAGAGTCCGTTTGGTAACTCAAATCCAATGGATATTTCAGATGATGAT
CTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

60.465

100

0.638

  ssbA Bacillus subtilis subsp. subtilis str. 168

58.286

100

0.626

  ssbB Streptococcus sobrinus strain NIDR 6715-7

55.963

66.871

0.374

  ssbB Bacillus subtilis subsp. subtilis str. 168

56.604

65.031

0.368