Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PG915_RS02655 Genome accession   NZ_CP115920
Coordinates   580355..581395 (-) Length   346 a.a.
NCBI ID   WP_353497758.1    Uniprot ID   A0AAU8BJI2
Organism   Vibrio chaetopteri strain CB1-14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 575355..586395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG915_RS02620 (PG915_02615) - 576835..578457 (+) 1623 WP_353497756.1 methyl-accepting chemotaxis protein -
  PG915_RS02650 (PG915_02645) pilU 579236..580342 (-) 1107 WP_418642298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG915_RS02655 (PG915_02650) pilT 580355..581395 (-) 1041 WP_353497758.1 type IV pilus twitching motility protein PilT Machinery gene
  PG915_RS02660 (PG915_02655) - 581421..582131 (+) 711 WP_353497759.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG915_RS02665 (PG915_02660) proC 582170..582988 (+) 819 WP_353497760.1 pyrroline-5-carboxylate reductase -
  PG915_RS02670 (PG915_02665) - 583020..583577 (+) 558 WP_353497761.1 YggT family protein -
  PG915_RS02675 (PG915_02670) yggU 583577..583864 (+) 288 WP_353497762.1 DUF167 family protein YggU -
  PG915_RS02680 (PG915_02675) - 583889..584320 (+) 432 WP_353497763.1 DUF4426 domain-containing protein -
  PG915_RS02685 (PG915_02680) - 584337..584939 (+) 603 WP_353497764.1 XTP/dITP diphosphatase -
  PG915_RS02690 (PG915_02685) hemW 584943..586112 (+) 1170 WP_353497765.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38382.83 Da        Isoelectric Point: 6.4490

>NTDB_id=774442 PG915_RS02655 WP_353497758.1 580355..581395(-) (pilT) [Vibrio chaetopteri strain CB1-14]
MDITELLDFSVKHNASDLHLSAGVSPMIRIDGEVRKLGIPALEHSEVHKLIFDIMNDKQRNEYQEHLEIDFSFELPNIGR
FRANAFNQSRGCSAVFRTIPTEIPTLDSIGAPSKFAELANLENGLVLVTGPTGSGKSTTLAAMVNHINEHQNKHILTIED
PIEFIHKNKKCLVNQREVHRDTHSFKSALRSALREDPDIILVGELRDQETISLALTAAETGHLVFATMHTNSAAKSVDRI
VDVFSGQDKDMVRSMLSESLRAVIAQTLLKRLGGGRVACHEIMVGTPAIRNLIREDKVAQMFSVIQTGAANGMQTMEQHA
RQLLSQGLIDIEEIGKKVVQNDNLFQ

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=774442 PG915_RS02655 WP_353497758.1 580355..581395(-) (pilT) [Vibrio chaetopteri strain CB1-14]
ATGGATATCACCGAGTTACTAGACTTCAGTGTAAAACATAATGCATCAGATCTACACCTTTCTGCAGGGGTTTCCCCCAT
GATACGCATCGATGGAGAAGTGAGAAAACTGGGCATCCCGGCGTTAGAGCATTCTGAAGTTCATAAACTCATCTTTGATA
TCATGAATGACAAGCAGCGCAACGAGTACCAAGAGCATTTAGAAATTGACTTTTCGTTTGAGCTACCCAATATCGGCCGC
TTTCGCGCCAATGCGTTTAATCAATCACGCGGCTGCTCAGCCGTGTTTCGAACTATCCCGACCGAAATACCGACACTCGA
CAGTATTGGTGCACCGAGCAAGTTTGCCGAGCTTGCGAACCTCGAGAATGGACTAGTACTGGTAACCGGGCCAACTGGCT
CAGGTAAATCCACCACACTCGCAGCTATGGTCAATCACATCAACGAACATCAGAACAAGCACATCTTGACCATTGAAGAT
CCTATCGAATTTATTCATAAAAACAAAAAGTGCCTGGTAAATCAACGAGAAGTGCACCGGGATACTCATAGCTTTAAATC
CGCGCTTCGCTCTGCTCTGAGGGAAGACCCCGATATTATTCTTGTCGGCGAGCTTCGCGACCAAGAGACCATTAGCCTTG
CGTTGACAGCGGCTGAAACCGGGCACTTAGTGTTTGCCACTATGCACACCAACTCTGCGGCTAAGTCTGTAGATAGGATA
GTTGATGTGTTTTCTGGCCAAGATAAAGACATGGTTCGCTCTATGCTCTCTGAGTCACTGCGAGCCGTCATTGCACAAAC
ACTACTTAAGCGCCTAGGGGGTGGCCGAGTTGCGTGTCATGAAATCATGGTTGGTACCCCAGCGATACGTAACCTCATCA
GAGAAGACAAGGTGGCTCAAATGTTTTCGGTTATTCAGACTGGAGCTGCGAATGGAATGCAAACCATGGAGCAGCACGCA
AGGCAGCTTCTTTCTCAAGGCCTAATCGATATTGAAGAGATCGGTAAGAAAGTGGTTCAAAATGACAATCTGTTTCAGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

79.586

97.688

0.777
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.586

97.688

0.777
  pilT Acinetobacter baylyi ADP1

69.789

95.665

0.668
  pilT Pseudomonas stutzeri DSM 10701

66.57

99.422

0.662
  pilT Acinetobacter nosocomialis M2

69.605

95.087

0.662
  pilT Acinetobacter baumannii strain A118

69.301

95.087

0.659
  pilT Acinetobacter baumannii D1279779

69.301

95.087

0.659
  pilT Pseudomonas aeruginosa PAK

66.279

99.422

0.659
  pilT Legionella pneumophila strain Lp02

65.217

99.711

0.65
  pilT Legionella pneumophila strain ERS1305867

65.217

99.711

0.65
  pilT Neisseria meningitidis 8013

62.865

98.844

0.621
  pilT Neisseria gonorrhoeae MS11

62.573

98.844

0.618
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.299

96.532

0.486
  pilU Vibrio cholerae strain A1552

41.538

93.931

0.39
  pilU Pseudomonas stutzeri DSM 10701

39.82

96.532

0.384
  pilU Acinetobacter baylyi ADP1

40.938

92.486

0.379