Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   M892_RS12440 Genome accession   NC_022269
Coordinates   2691731..2693479 (+) Length   582 a.a.
NCBI ID   WP_041853271.1    Uniprot ID   -
Organism   Vibrio campbellii ATCC BAA-1116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2686731..2698479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M892_RS12420 (M892_12460) pilM 2689030..2690046 (+) 1017 WP_041853270.1 type IV pilus assembly protein PilM Machinery gene
  M892_RS12425 (M892_12465) pilN 2690030..2690605 (+) 576 WP_012126434.1 PilN domain-containing protein Machinery gene
  M892_RS12430 (M892_12470) pilO 2690598..2691191 (+) 594 WP_012126433.1 type 4a pilus biogenesis protein PilO Machinery gene
  M892_RS12435 (M892_12475) pilP 2691181..2691696 (+) 516 WP_012126432.1 pilus assembly protein PilP Machinery gene
  M892_RS12440 (M892_12480) pilQ 2691731..2693479 (+) 1749 WP_041853271.1 type IV pilus secretin PilQ family protein Machinery gene
  M892_RS12445 (M892_12485) aroK 2693668..2694186 (+) 519 WP_010443904.1 shikimate kinase AroK -
  M892_RS12450 (M892_12490) aroB 2694218..2695318 (+) 1101 WP_012126430.1 3-dehydroquinate synthase -
  M892_RS12455 (M892_12495) - 2695372..2696865 (+) 1494 WP_012126429.1 AAA family ATPase -
  M892_RS12460 (M892_12500) - 2696935..2697774 (+) 840 WP_005532700.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63814.76 Da        Isoelectric Point: 4.5130

>NTDB_id=61966 M892_RS12440 WP_041853271.1 2691731..2693479(+) (pilQ) [Vibrio campbellii ATCC BAA-1116]
MKMRHGLTLTPRKLKGLFSLWFFLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVQDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGDDGGSSVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=61966 M892_RS12440 WP_041853271.1 2691731..2693479(+) (pilQ) [Vibrio campbellii ATCC BAA-1116]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAAAAGGGCTCTTCTCTTTATGGTTCTTTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCAGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGCAAGATTTCTCGACAGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATAGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTATAACCAATTTAACTTGGTCGTCTC
TGACTCGGTTGAGGGTAACTTGACCTTGCGTTTGGATGGTGTGCCTTGGCAGCAAGTACTCGACATTATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTTGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGGGAGGGCAACATCAGTATGCTTTCTGATCGAGGCAGCATGACCATTGATGAGCGAACAA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACTTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCCTGTACGATGGCGGCGGTGATG
GTGAAGGTGATGACGGTGGCAGCAGTGTGGGTATTGACGACTTTCTCAACGTTAACCTTGCTGCGACCAGTCCAAATGCG
ACCAGTATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATC
GAAAGCAGAAATTATTTCCAGTCCACGTTTGATCACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCC
CTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACGCCACAG
ATTACGCCTGATAACCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGG
TGAGGCGGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTTAATAATGGCGAGACAGTCGTGTTGGGCGGTA
TTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCGT
CGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

98.797

100

0.988
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.289

97.595

0.754
  pilQ Vibrio cholerae strain A1552

77.289

97.595

0.754
  pilQ Legionella pneumophila strain ERS1305867

41.742

94.674

0.395
  pilQ Legionella pneumophila strain Lp02

41.742

94.674

0.395
  pilQ Pseudomonas aeruginosa PAK

40.142

96.735

0.388
  pilQ Acinetobacter baumannii D1279779

37.271

100

0.385
  pilQ Acinetobacter baumannii strain A118

37.271

100

0.385
  comQ Acinetobacter baylyi ADP1

37.005

99.828

0.369

Multiple sequence alignment