Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H681_RS04965 Genome accession   NC_020829
Coordinates   1073445..1075148 (-) Length   567 a.a.
NCBI ID   WP_041711736.1    Uniprot ID   -
Organism   Pseudomonas sp. ATCC 13867     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1068445..1080148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H681_RS04930 (H681_04900) - 1068857..1069324 (+) 468 WP_041711734.1 hypothetical protein -
  H681_RS04935 (H681_04905) - 1069321..1070010 (+) 690 WP_015475733.1 energy-coupling factor ABC transporter permease -
  H681_RS04940 (H681_04910) - 1070117..1070332 (+) 216 WP_015475734.1 hypothetical protein -
  H681_RS04945 (H681_04915) yacG 1070472..1070675 (-) 204 WP_015475735.1 DNA gyrase inhibitor YacG -
  H681_RS04950 (H681_04920) coaE 1070672..1071280 (-) 609 WP_015475736.1 dephospho-CoA kinase -
  H681_RS04955 (H681_04925) pilD 1071346..1072218 (-) 873 WP_015475737.1 A24 family peptidase Machinery gene
  H681_RS04960 (H681_04930) pilC 1072224..1073441 (-) 1218 WP_015475738.1 type II secretion system F family protein Machinery gene
  H681_RS04965 (H681_04935) pilB 1073445..1075148 (-) 1704 WP_041711736.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H681_RS04970 (H681_04940) - 1075374..1075835 (+) 462 WP_015475740.1 pilin -
  H681_RS26320 (H681_04945) - 1075927..1077852 (+) 1926 WP_015475741.1 hypothetical protein -
  H681_RS25655 - 1077836..1078321 (+) 486 WP_157883298.1 GtrA family protein -
  H681_RS04985 (H681_04955) - 1078311..1079855 (+) 1545 WP_041711738.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62677.93 Da        Isoelectric Point: 5.8095

>NTDB_id=57451 H681_RS04965 WP_041711736.1 1073445..1075148(-) (pilB) [Pseudomonas sp. ATCC 13867]
MNDQVVLSGLARQLVLSQLLDENSARQAQLQAQRNKLSLVTYLVQNKLIKSRPLMELAADQFGVAFCDLSSLDRESFPKE
LVSEKLVRQHRVLPLWRRGNKLFVGLSDPTNHQAITDIQFSTGLTTEAILVEDDRLGDAIEKLFESVTGGLDDLGDVDLE
GLDIESGNAERKEEAIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLQEVAKPPVQLVSRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEEEQKQLYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKTGLDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPACKKEHDVPRETLLHEGFP
EARIGSFKLYAPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
INRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=57451 H681_RS04965 WP_041711736.1 1073445..1075148(-) (pilB) [Pseudomonas sp. ATCC 13867]
ATGAACGATCAAGTCGTCCTGTCCGGCCTTGCCCGGCAGCTGGTGTTGTCTCAACTGCTCGATGAGAACAGCGCGCGGCA
AGCCCAGCTACAGGCGCAACGCAATAAGCTGTCGTTGGTGACCTATCTCGTACAGAACAAATTGATAAAAAGCCGTCCAT
TGATGGAGTTGGCCGCCGACCAGTTCGGTGTCGCCTTCTGCGATCTGAGTAGCCTGGACCGCGAGAGCTTTCCCAAGGAG
CTGGTCAGCGAGAAACTCGTTCGCCAGCACCGAGTCCTCCCCTTGTGGCGGAGAGGTAATAAGCTCTTTGTTGGACTGTC
AGACCCTACCAACCATCAGGCAATCACCGACATCCAATTCAGCACCGGACTGACCACCGAAGCCATCCTGGTCGAAGACG
ACCGGCTCGGCGATGCCATCGAAAAGCTCTTCGAGAGCGTTACCGGCGGATTGGATGACCTGGGCGACGTCGATCTGGAA
GGCTTGGATATCGAGAGCGGCAATGCCGAGCGCAAGGAGGAAGCCATCGGCGGCGACGCCGACGACGCCCCGGTGGTGCG
CTTCGTGAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCGTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACC
GCGTTCGCTTCCGCACCGACGGCATGCTCCAGGAAGTCGCCAAGCCGCCGGTCCAACTGGTCAGCCGCATCTCCGCCCGC
CTCAAGGTCATGGCTGGCCTGGATATCTCCGAGCGCCGCAAGCCCCAGGACGGTCGGATCAAGATGCGCGTATCCAAGAC
CAAGTCCATCGACTTCCGCGTCAATACCCTGCCCACGCTGTGGGGCGAAAAGATCGTGATGCGTATCCTCGACTCGGCCA
GTGCGCAAATGGGCATCGACGCTCTGGGCTATGAGGAAGAGCAGAAGCAGCTTTATCTTTCGGCACTCAAGCAGCCGCAG
GGCATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACAGTATCCCTGTATACCGGCCTGAATATCCTCAACACCCC
GGACATCAACATCTCGACCGCGGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGAAGA
CAGGCCTGGACTTCGCCCAGGCACTGCGTGCCTTCCTGCGCCAGGACCCTGACGTGATCATGGTGGGCGAGATCCGCGAC
CTCGAGACCGCAGAAATCGCCATCAAGGCAGCCCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTCACCCGCCTGCTGAACATGGGCGTGGCTTCCTTCAACCTCGCCACCTCCACCAACCTGATCATTGCCCAGC
GCCTGGCCAGAAAACTGTGCCCGGCCTGCAAGAAGGAGCACGACGTTCCACGCGAAACGCTGCTCCATGAAGGCTTCCCG
GAAGCCAGGATCGGTTCGTTCAAGCTCTACGCCCCGGTCGGGTGCGAAAACTGCAAGAACGGTTACAAGGGGCGTGTAGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTACAGCGGATTATCATGGAAGAAGGCAATTCCATTCAGATCGCCGAAC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCGGGCCTGCTGAAAGCCATGCAGGGAATTACCAGCCTCGAGGAA
ATCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.053

99.647

0.578
  pilB Acinetobacter baylyi ADP1

57.801

99.471

0.575
  pilB Legionella pneumophila strain ERS1305867

54.674

100

0.547
  pilB Vibrio parahaemolyticus RIMD 2210633

52.126

95.414

0.497
  pilF Neisseria gonorrhoeae MS11

49.735

100

0.497
  pilB Vibrio campbellii strain DS40M4

51.577

95.062

0.49
  pilB Vibrio cholerae strain A1552

52.264

93.474

0.489
  pilF Thermus thermophilus HB27

38.421

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.587

94.004

0.372

Multiple sequence alignment